公开(公告)号：US20050003507A1

公开(公告)日：2005-01-06

申请号：US10869143

申请日：2004-06-16

申请人： Paul Kostel ,  Kurt Yanagimachi ,  Charles Olson

发明人： Paul Kostel ,  Kurt Yanagimachi ,  Charles Olson

IPC分类号： A61K20060101 ,  C12N7/02

CPC分类号： C12N7/00 ,  C12N2710/10351

摘要： A process for the purification of viruses from a cell lysate preparation is described, consisting of preferably two successive chromatographic steps; the first a clarification step utilizing size exclusion chromatography, and the second, a virus capture and release step using anion exchange chromatography, which successive chromatographic steps have the advantage of purifying virus, and avoiding chromatography buffer conductivity adjustments.

摘要翻译： 描述了从细胞裂解物制剂中纯化病毒的方法，其优选包括两个连续的色谱步骤; 第一个使用尺寸排阻色谱的澄清步骤，第二个是使用阴离子交换色谱法的病毒捕获和释放步骤，其连续的色谱步骤具有净化病毒的优点，并避免色谱缓冲液电导率调节。

公开(公告)号：US20060258947A1

公开(公告)日：2006-11-16

申请号：US11411365

申请日：2006-04-25

申请人： Charles Olson

发明人： Charles Olson

IPC分类号： A61B5/0402

CPC分类号： A61B5/044 ,  A61B5/04011

摘要： A medical display for analyzing heart signals includes a cardiographic display which displays an electrocardiograph (ECG) heart signal segment of a patient having a magnitude and location in vector format within a single three-dimensional (3D) coordinate system (vectorcardiograph) sampled at incremental time intervals. The display communicates with a central processing unit (CPU) that implements an algorithm to permit a user to selectively and visually display a comparison of the patient ECG with at least one known display in vector format within a single three-dimensional (3D) coordinate system. The display also permits a user to selectively and visually convert and display an ECG heart signal segment into a color-coded projection of a time sequence, A method for analyzing heart signals includes implementing the algorithm to selectively and visually compare the ECG heart signal with at least one known display in vector format selected from the group.

摘要翻译： 用于分析心脏信号的医学显示器包括心电图显示器，其以在增量时间采样的单个三维（3D）坐标系（矢量心电图仪）内显示具有向量格式的幅度和位置的患者的心电图（ECG）心脏信号段 间隔 显示器与实现算法的中央处理单元（CPU）进行通信，以允许用户在单个三维（3D）坐标系统内选择性地和可视地显示患者ECG与矢量格式中的至少一个已知显示的比较 。 该显示还允许用户选择性地和可视地将ECG心脏信号段转换并显示为时间序列的彩色编码投影。用于分析心脏信号的方法包括实施该算法以选择性地和视觉地比较ECG心脏信号与at 以从组中选择的向量格式中的至少一个已知显示。

公开(公告)号：US06214975B1

公开(公告)日：2001-04-10

申请号：US09466711

申请日：1999-12-17

申请人： Michael Zachariou ,  Jonathan Mazer ,  Hyun J. Park ,  Charles Olson

发明人： Michael Zachariou ,  Jonathan Mazer ,  Hyun J. Park ,  Charles Olson

IPC分类号： C07K1700

CPC分类号： C07K14/755 ,  Y10S435/813 ,  Y10S435/814

摘要： A chemiluminescence-based gel assay may be used as an indicator of fermenter health. The assay is performed using a sample of fermentation medium essentially directly from the fermenter (with no need for complicated purification of the sample before performing the assay). Results from the assay may be correlated with the final product purification yield of a given protein/purification protocol system. This may then be used as a predictor of purification yield before actually performing the purification.

摘要翻译： 基于化学发光的凝胶测定可用作发酵罐健康的指标。 使用基本上直接从发酵罐发酵培养基的样品进行测定（在进行测定之前不需要对样品进行复杂的纯化）。 测定结果可能与给定蛋白质/纯化方案系统的最终产物纯化产率相关。 然后可以在实际进行纯化之前将其用作纯化产率的预测因子。

公开(公告)号：US20140259828A1

公开(公告)日：2014-09-18

申请号：US14202792

申请日：2014-03-10

申请人： Charles Olson

发明人： Charles Olson

IPC分类号： G09F13/00

CPC分类号： G09F13/005 ,  G09F9/33 ,  G09F13/22 ,  G09F19/228 ,  G09F2013/0459 ,  G09F2013/0495 ,  G09F2013/222

摘要： An improved fire exit sign is installed at the floor-level threshold of an emergency exit doorway, and is provided with a set of Light-Emitting-Diodes (LEDs), preferably red, embedded in transparent epoxy. The lights would be wired into the emergency illumination system in a building, and may optionally be configured to blink. The epoxy, and the cover and frame, would be strong enough to sustain the weight of persons moving through the doorway.

摘要翻译： 在紧急出口门口的地板级门槛处安装了改进的防火标志，并且设置有一组嵌入透明环氧树脂的发光二极管（LED），最好为红色。 灯将被连接到建筑物中的应急照明系统中，并且可以可选地被配置为闪烁。 环氧树脂和盖子和框架将足够坚固以维持移动通过门口的人的重量。

公开(公告)号：US06338964B1

公开(公告)日：2002-01-15

申请号：US09307175

申请日：1999-05-07

申请人： Ricaredo Matanguihan ,  Eva Sajan ,  Konstantin Konstantinov ,  Michael Zachariou ,  Charles Olson

发明人： Ricaredo Matanguihan ,  Eva Sajan ,  Konstantin Konstantinov ,  Michael Zachariou ,  Charles Olson

IPC分类号： C12N500

CPC分类号： C12N5/0018 ,  C12N2500/10 ,  C12N2500/32 ,  C12N2500/60

摘要： A cell culture medium which is low in dissolved carbon dioxide is disclosed. The medium contains less than about 1 g/L added sodium bicarbonate and includes an organic buffer and a metal complexing agent. The medium is preferably essentially free of added sodium bicarbonate. Methods of use of the medium in culturing mammalian cells, particularly cells engineered to produce recombinant factor VIII, are also disclosed.

摘要翻译： 公开了一种低溶解二氧化碳的细胞培养基。 所述培养基含有少于约1g / L的碳酸氢钠，并且包括有机缓冲剂和金属络合剂。 该介质优选基本上不含加入的碳酸氢钠。 还公开了培养哺乳动物细胞，特别是工程改造以产生重组因子VIII的细胞的培养基的使用方法。

公开(公告)号：US5803084A

公开(公告)日：1998-09-08

申请号：US760986

申请日：1996-12-05

申请人： Charles Olson

发明人： Charles Olson

IPC分类号： A61B5/04 ,  G06F17/00 ,  A61B5/0402

CPC分类号： A61B5/04011 ,  A61B5/044

摘要： A cardiographic display for displaying an electrocardiograph heart signal in vector format within a single three-dimensional coordinate system sampled at incremental time intervals and combining the above vector display on the same screen with other displays, e.g., a 12-Lead display. Other embodiments of the present invention comprise projecting the results of the heart vectors onto three planes of the coordinate system which represent the frontal, transverse and sagittal planes while simultaneously displaying the three-dimensional vector display. Still other embodiments comprise combining the three-dimensional with various graphs which show the various changes in magnitude and angle between the heart vectors.

摘要翻译： 一种心电图显示器，用于在以增量的时间间隔采样的单个三维坐标系中以矢量格式显示心电图仪心脏信号，并将同一屏幕上的上述矢量显示与其他显示器（例如12-Lead显示器）组合。 本发明的其它实施例包括将心脏矢量的结果投影到坐标系的三个平面上，该坐标系表示正面，横向和矢状面，同时显示三维矢量显示。 其他实施例包括将三维与各种图形组合，其中显示了心脏矢量之间的大小和角度的各种变化。

公开(公告)号：US08412314B2

公开(公告)日：2013-04-02

申请号：US12710943

申请日：2010-02-23

申请人： Charles Olson

发明人： Charles Olson

IPC分类号： A61B5/0402

CPC分类号： A61B5/04011 ,  A61B5/044

摘要： A method for locating an ischemic region in the heart of a subject includes establishing three dimensional coordinates axes with respect to the torso of the subject as a reference; establishing as a reference a multi-dimensional representation of the heart defining at least three dimensional coordinate axes of the heart, the multi-dimensional representation defining at least the base of the heart and a middle section of the heart to thereby prescribe a surface of the heart on the reference multi-dimensional representation of the heart; and orienting the three dimensional coordinate axes of the heart from an initial position offset with respect to the three dimensional coordinates with respect to the torso of the subject to an imaginary position wherein at least one axis of the heart is parallel to or coincident with at least one of the three dimensional coordinate axes with respect to the torso of the subject. Corresponding displays are disclosed also.

摘要翻译： 用于定位受试者心脏中的缺血区域的方法包括建立相对于受试者的躯干的三维坐标轴作为参考; 将作为心脏的至少三维坐标轴的心脏的多维表示建立为参考，所述多维表示至少限定心脏的基部和心脏的中间部分，从而规定了心脏的表面 心脏参考心脏的多维表示; 并且将心脏的三维坐标轴相对于相对于受试者的躯干的三维坐标偏移的初始位置定向到假想位置，其中心脏的至少一个轴线至少平行于或与至少一致 相对于被摄体的躯干的三维坐标轴之一。 也公开了相应的显示。

公开(公告)号：US07751874B2

公开(公告)日：2010-07-06

申请号：US11411365

申请日：2006-04-25

申请人： Charles Olson

发明人： Charles Olson

IPC分类号： A61B5/04 ,  A61B5/044

CPC分类号： A61B5/044 ,  A61B5/04011

摘要： A medical display for analyzing heart signals includes a cardiographic display which displays an electrocardiograph (ECG) heart signal segment of a patient having a magnitude and location in vector format within a single three-dimensional (3D) coordinate system (vectorcardiograph) sampled at incremental time intervals. The display communicates with a central processing unit (CPU) that implements an algorithm to permit a user to selectively and visually display a comparison of the patient ECG with at least one known display in vector format within a single three-dimensional (3D) coordinate system. The display also permits a user to selectively and visually convert and display an ECG heart signal segment into a color-coded projection of a time sequence, A method for analyzing heart signals includes implementing the algorithm to selectively and visually compare the ECG heart signal with at least one known display in vector format selected from the group.

摘要翻译： 用于分析心脏信号的医学显示器包括心电图显示器，其以在增量时间采样的单个三维（3D）坐标系（矢量心电图仪）内显示具有向量格式的幅度和位置的患者的心电图（ECG）心脏信号段 间隔 显示器与实现算法的中央处理单元（CPU）进行通信，以允许用户在单个三维（3D）坐标系统内选择性地和可视地显示患者ECG与矢量格式中的至少一个已知显示的比较 。 该显示还允许用户选择性地和可视地将ECG心脏信号段转换并显示为时间序列的彩色编码投影。用于分析心脏信号的方法包括实施该算法以选择性地和视觉地比较ECG心脏信号与at 以从组中选择的向量格式中的至少一个已知显示。

公开(公告)号：US5475707A

公开(公告)日：1995-12-12

申请号：US186407

申请日：1994-02-28

申请人： Joseph A. Ficarra ,  Charles Olson

发明人： Joseph A. Ficarra ,  Charles Olson

IPC分类号： H04B1/69 ,  H04J13/00

CPC分类号： H04J13/00 ,  H04B1/69

摘要： A signal of bandwidth F.sub.max is unambiguously transmitted over a datalink having a bandwidth less than F.sub.max by subdividing the signal into a plurality of subspectrums and bandshifting the subspectrums greater than the datalink bandwidth to baseband. Each bandshifted subspectrum is uniquely encoded using a different pilot tone which can be detected by the receiver to decode the receive signal and unambiguously recover the narrowband spectrum components.

摘要翻译： 带宽Fmax的信号通过将信号细分为多个子频带并将子频带大于数据链路带宽的频带移位到基带，通过具有小于Fmax的带宽的数据链路被明确地发送。 使用不同的导频音来对每个带移频子频道进行唯一的编码，接收机可以检测该导频音以对接收信号进行解码，并明确地恢复窄带频谱分量。