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6 条记录 (耗时 0.018 秒)

    Method of gene delivery using wildtype adeno associated viral (AAV) vectors with insertions
    1.
    发明授权
    Method of gene delivery using wildtype adeno associated viral (AAV) vectors with insertions 失效
    使用具有插入的野生型腺相关病毒（AAV）载体的基因递送方法

    公开(公告)号：US6153436A

    公开(公告)日：2000-11-28

    申请号：US4877

    申请日：1998-01-09

    申请人： Paul L. Hermonat ,  J. Gerald Quirk ,  Brian Bishop ,  Han Li

    发明人： Paul L. Hermonat ,  J. Gerald Quirk ,  Brian Bishop ,  Han Li

    IPC分类号： A61K48/00 ,  C12N15/864 ,  C12N15/86

    CPC分类号： C12N15/86 ,  A61K48/00 ,  C12N2750/14143

    摘要： The present invention provides a method of human gene therapy using AAV vectors with essentially wildtype phenotype. Genes of 900 bases or less can be inserted into wildtype AAV and still allow the resulting vector to have a largely wildtype phenotype. For example, several antisense genes could be inserted and still allow packaging. Such wildtype vectors have several advantages. First, high titers of such vectors is easy to accomplish. Second, the vectors, since they include the Rep78 gene, integrate specifically into human chromosome 19. Third, such vectors, being wildtype, spread after their initial introduction. Another method for use of large wildtype AAV genomes is as complementors for fully defective AAV vectors. Such complementors can be delivered by virus infection and, be introduced easily into 100% of the cells used to produce virus. Viral infection is superior to synthetic techniques for introducing DNA into tissue culture producer cells. When large essentially wildtype AAV complementor virus are used in conjunction with AAV vector virus allowing for the introduction of both vectors into all cells, high titers of recombinant AAV virus is achieved.

    摘要翻译： 本发明提供使用基本上野生型表型的AAV载体的人基因治疗方法。 将900个碱基以下的基因插入到野生型AAV中，并且仍然允许所得载体具有很大的野生型表型。 例如，可以插入几个反义基因并仍然允许包装。 这种野生型载体具有几个优点。 首先，这种载体的高滴度很容易完成。 第二，载体，因为它们包括Rep78基因，特异性整合入人染色体19。第三，这种载体，野生型，在其初次引入后扩散。 使用大型野生型AAV基因组的另一种方法是完全缺陷的AAV载体的补体。 这样的补体可以通过病毒感染递送，并且容易地引入用于产生病毒的100％的细胞中。 病毒感染优于将DNA引入组织培养生产细胞的合成技术。 当大的基本野生型AAV补体病毒与AAV载体病毒结合使用时，允许将两种载体引入所有细胞，实现了高效价的重组AAV病毒。

    Floating Point Intensive Reconfigurable Computing System for Iterative Applications
    4.
    发明申请
    Floating Point Intensive Reconfigurable Computing System for Iterative Applications 审中-公开
    浮点密集可重构计算系统的迭代应用

    公开(公告)号：US20070067380A2

    公开(公告)日：2007-03-22

    申请号：US10862269

    申请日：2004-06-07

    申请人： Brian Bishop ,  Thomas Kelliher ,  Shrirang Yardi

    发明人： Brian Bishop ,  Thomas Kelliher ,  Shrirang Yardi

    IPC分类号： G06F15/00

    CPC分类号： G06F9/3885 ,  G06F9/3887 ,  G06F15/8023

    摘要： A reconfigurable computing system for accelerating execution of floating point intensive iterative applications. The reconfigurable computing system includes a plurality of interconnected processing elements mounted on a printed circuit board, a host processing system for displaying real-time outputs of the floating point calculations performed by the processing elements, and an interface for connecting the processing elements to the host system. Each of the interconnected processing elements includes a floating point functional unit, operand memory, control memory and a control unit. The floating point functional unit includes a multiply accumulate function. The operand memory includes a plurality of banks of static random access memory. The processing elements are interconnected using a nearest neighbor or hierarchical implementation. The instruction set performed by the floating point functional unit includes arithmetic, control and communication instructions. The interface can be implemented as a PCI bus interface using a field programmable gate array or as an AGP bus interface.

    摘要翻译： 用于加速浮点密集迭代应用程序执行的可重构计算系统。 可重构计算系统包括安装在印刷电路板上的多个互连的处理元件，用于显示由处理元件执行的浮点计算的实时输出的主处理系统，以及用于将处理元件连接到主机 系统。 每个互连的处理元件包括浮点功能单元，操作数存储器，控制存储器和控制单元。 浮点功能单元包括乘法累加函数。 操作数存储器包括多个静态随机存取存储器组。 处理元件使用最近邻或分层实现互连。 由浮点功能单元执行的指令集包括算术，控制和通信指令。 该接口可以实现为使用现场可编程门阵列或AGP总线接口的PCI总线接口。

    Dual area piston for transmission clutch and sequential control therefor
    6.
    发明授权
    Dual area piston for transmission clutch and sequential control therefor 有权
    双面活塞用于传动离合器和顺序控制

    公开(公告)号：US07104380B2

    公开(公告)日：2006-09-12

    申请号：US10942071

    申请日：2004-09-15

    申请人： Brian Bishop ,  Pete Bezjak ,  Karl Jungbluth

    发明人： Brian Bishop ,  Pete Bezjak ,  Karl Jungbluth

    IPC分类号： F16D25/638 ,  F16D25/12

    CPC分类号： F16D25/0638 ,  F16D48/02 ,  F16D2048/0212

    摘要： A system for actuating a clutch that alternately driveably connects and disconnects components, includes a clutch having a piston that includes a first apply area and a second apply area, a fluid pressure source, a source of variable control pressure, and a control coupled with the fluid pressure source and operative in response to the control pressure to engage the clutch initially by increasing pressure steadily at the first apply area up to a first magnitude followed by a rapid increase in pressure at the first apply area and the second apply area above the first magnitude to a second magnitude.

    摘要翻译： 用于致动交替驱动地连接和断开部件的离合器的系统包括具有活塞的离合器，该活塞包括第一施加区域和第二施加区域，流体压力源，可变控制压力源和与 流体压力源并且响应于控制压力而起作用，起初通过在第一施加区域处稳定地增加压力直到第一量级，然后在第一施加区域和第一施加区域上方的第二施加区域的第一应用区域上的压力快速增加， 大小到第二大。