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13 条记录 (耗时 0.025 秒)

    Polynucleotide barcoding
    1.
    发明授权
    Polynucleotide barcoding 有权
    多核苷酸条形码

    公开(公告)号：US07829278B2

    公开(公告)日：2010-11-09

    申请号：US10976546

    申请日：2004-10-28

    申请人： Paul R. Selvin ,  Matthew Gordon ,  Pui-Yan Kwok ,  Ming Xiao ,  Ting-Fung Chan

    发明人： Paul R. Selvin ,  Matthew Gordon ,  Pui-Yan Kwok ,  Ming Xiao ,  Ting-Fung Chan

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6858 ,  C12Q1/6827

    摘要： A polynucleotide is barcoded using a method whereby an isolated, individual polynucleotide is immobilized on a solid phase and stretched, targets are labeled using target-specific hybridization probes, and an individual label of an unamplified probe at each of the labeled targets is optically detected. The order of the labels is determined to form a barcode representation of the polynucleotide wherein the targets and their relative positions are represented.

    摘要翻译： 使用这样一种方法对多核苷酸进行条形码化，由此将分离的单个多核苷酸固定在固相上并拉伸，使用靶特异性杂交探针对靶进行标记，并光学检测每个标记靶上的未扩增探针的单个标记。 确定标签的顺序以形成多核苷酸的条形码表示，其中表示靶标及其相对位置。

    Semiconductor luminescence quenchers for detecting proximal molecular binding events
    2.
    发明授权
    Semiconductor luminescence quenchers for detecting proximal molecular binding events 有权
    用于检测近端分子结合事件的半导体发光猝灭剂

    公开(公告)号：US06667179B1

    公开(公告)日：2003-12-23

    申请号：US09430384

    申请日：1999-10-28

    申请人： Paul R. Selvin

    发明人： Paul R. Selvin

    IPC分类号： G01N33557

    CPC分类号： G01N33/5438 ,  G01N33/542 ,  Y10S436/805

    摘要： The invention provides methods and compositions for detecting binding or unbinding of a molecule to a substrate. The molecule comprises a luminophore and the substrate comprises a semiconductor which acts as a luminescence quencher to provide distance-dependent quenching of the luminophore. Binding or unbinding of the molecule, which may be covalent or noncovalent, is detected as a decrease or increase, respectively, of the detectable luminescence of the luminophore.

    摘要翻译： 本发明提供了用于检测分子与底物的结合或解离的方法和组合物。 该分子包含发光体，并且该衬底包含用作发光猝灭剂以提供发光体距离相关猝灭的半导体。 可以分析可能共价或非共价分子的分子的结合或解离，分别是发光体的可检测发光的降低或增加。

    Luminescent lanthanide chelates and methods of use
    3.
    发明授权
    Luminescent lanthanide chelates and methods of use 失效

    公开(公告)号：US5622821A

    公开(公告)日：1997-04-22

    申请号：US269162

    申请日：1994-06-29

    申请人： Paul R. Selvin ,  John Hearst

    发明人： Paul R. Selvin ,  John Hearst

    IPC分类号： C07F5/00 ,  C09K3/00 ,  C09K11/06 ,  G01N33/533 ,  G01N33/542 ,  G01N33/58 ,  C12Q1/68

    CPC分类号： G01N33/542 ,  G01N33/533 ,  G01N33/582 ,  Y10S435/81 ,  Y10S436/80 ,  Y10S436/805 ,  Y10T436/13

    摘要： The invention provides lanthanide chelates capable of intense luminescence. The celates comprise a lanthanide chelator covalently joined to a coumarin-like or quinolone-like sensitizer. Exemplary sensitzers include 2- or 4-quinolones, 2- or 4-coumarins, or derivatives thereof e.g. carbostyril 124 (7-amino-4-methyl-2-quinolone), coumarin 120 (7-amino-4-methyl-2-coumarin), coumarin 124 (7-amino-4-(trifluoromethyl)-2-coumarin), aminomethyltrimethylpsoralen, etc.The chelates form high affinity complexes with lanthanides, such as terbium or europium, through chelator groups, such as DTPA. The chelates may be coupled to a wide variety of compounds to create specific labels, probes, diagnostic and/or therapeutic reagents, etc. The chelates find particular use in resonance energy transfer between chelate-lanthanide complexes and another luminescent agent, often a fluorescent non-metal based resonance energy acceptor. The methods provide useful information about the structure, conformation, relative location and/or interactions of macromolecules.

    Photobleaching and intermittency localization microscopy
    4.
    发明授权
    Photobleaching and intermittency localization microscopy 有权
    漂白和间歇定位显微镜

    公开(公告)号：US08445867B2

    公开(公告)日：2013-05-21

    申请号：US13578218

    申请日：2011-02-22

    申请人： Paul R. Selvin ,  Paul Dennis Simonson

    发明人： Paul R. Selvin ,  Paul Dennis Simonson

    IPC分类号： G01J1/58

    CPC分类号： G01N21/6458 ,  G06T3/4069

    摘要： A method of image analysis creates super-resolution images from images with high densities of fluorophores by processing a movie in which the fluorescent molecules or particles are photobleaching or blinking. The method looks for the individual photobleaching events that can be located with high resolution (nm to tens of nanometers scale). The positions of the photobleaching or blinking events are then drawn in a composite image that is at a much higher resolution than the original fluorescence movie.

    摘要翻译： 图像分析的方法通过处理荧光分子或颗粒光漂白或闪烁的影片，从具有高密度荧光团的图像产生超分辨率图像。 该方法寻找可以以高分辨率（nm到数十纳米级）定位的各个光漂白事件。 然后将光漂白或闪烁事件的位置绘制在比原始荧光电影高得多的分辨率的合成图像中。

    Luminescent lanthanide chelates and methods of use
    5.
    发明授权
    Luminescent lanthanide chelates and methods of use 失效
    发光镧系元素螯合物和使用方法

    公开(公告)号：US5656433A

    公开(公告)日：1997-08-12

    申请号：US762288

    申请日：1996-12-09

    申请人： Paul R. Selvin ,  John Hearst

    发明人： Paul R. Selvin ,  John Hearst

    IPC分类号： C07F5/00 ,  C09K3/00 ,  C09K11/06 ,  G01N33/533 ,  G01N33/542 ,  G01N33/58 ,  C12Q1/68

    CPC分类号： G01N33/542 ,  G01N33/533 ,  G01N33/582 ,  Y10S435/81 ,  Y10S436/80 ,  Y10S436/805 ,  Y10T436/13

    摘要： The invention provides lanthanide chelates capable of intense luminescence. The celates comprise a lanthanide chelator covalently joined to a coumarin-like or quinolone-like sensitizer. Exemplary sensitizers include 2- or 4-quinolones, 2- or 4-coumarins, or derivatives thereof e.g. carbostyril 124 (7-amino-4-methyl-2-quinolone), coumarin 120 (7-amino-4-methyl-2-coumarin), coumarin 124 (7-amino-4-(trifluoromethyl)-2-coumarin), aminomethyltrimethylpsoralen, etc.The chelates form high affinity complexes with lanthanides, such as terbium or europium, through chelator groups, such as DTPA. The chelates may be coupled to a wide variety of compounds to create specific labels, probes, diagnostic and/or therapeutic reagents, etc. The chelates find particular use in resonance energy transfer between chelate-lanthanide complexes and another luminescent agent, often a fluorescent non-metal based resonance energy acceptor. The methods provide useful information about the structure, conformation, relative location and/or interactions of macromolecules.

    摘要翻译： 本发明提供能够强烈发光的镧系元素螯合物。 纤维素包含与香豆素样或类喹诺酮类敏化剂共价连接的镧系元素螯合剂。 示例性敏化剂包括2-或4-喹诺酮，2-或4-香豆素或其衍生物，例如 喹诺酮酮124（7-氨基-4-甲基-2-喹诺酮），香豆素120（7-氨基-4-甲基-2-香豆素），香豆素124（7-氨基-4-（三氟甲基）-2-香豆素） 氨基甲基三甲基螺杆菌等。螯合物通过螯合剂基团如DTPA与镧系元素如铽或铕形成高亲和性络合物。 螯合物可以与多种化合物偶联以产生特异性标记，探针，诊断和/或治疗试剂等。螯合物特别用于螯合镧系元素络合物与另一种发光剂之间的共振能量转移， 基于金属的共振能量受体。 该方法提供有关大分子的结构，构象，相对位置和/或相互作用的有用信息。

    Simple ultra-stable stage with built-in fiduciary markers for fluorescence nanoscopy
    9.
    发明授权
    Simple ultra-stable stage with built-in fiduciary markers for fluorescence nanoscopy 有权
    简单的超稳定阶段，内置荧光纳米视觉受信标记

    公开(公告)号：US08717673B2

    公开(公告)日：2014-05-06

    申请号：US13118400

    申请日：2011-05-28

    申请人： Paul R. Selvin ,  Sang Hak Lee

    发明人： Paul R. Selvin ,  Sang Hak Lee

    IPC分类号： G02B21/34 ,  G02B27/32 ,  G02B21/00

    CPC分类号： G02B21/34 ,  G02B21/00 ,  G02B21/16 ,  G02B21/26 ,  G02B27/32

    摘要： An improved microscope stage mount with built-in fiduciary markers is used for fluorescence microscopy, and comprises: (a) an optically-transparent glass plate adapted for specimen mounting and microscope viewing and comprising a specimen mounting area; and (b) a defined and ordered, two-dimensional microscopic array of fiduciary markers, wherein the markers are polymeric pillars affixed to the plate about the specimen mounting area, wherein the markers provide a three-dimensional spatial reference for the specimen.

    摘要翻译： 用于荧光显微镜的改进的具有内置受信标记的显微镜载置台，包括：（a）适于样品安装和显微镜观察的光学透明玻璃板，包括样品安装区域; 和（b）限定和有序的受托标记的二维微观阵列，其中所述标记是在所述样品安装区域周围固定在所述板上的聚合物柱，其中所述标记提供所述样品的三维空间参考。

    Photobleaching and Intermittency Localization Microscopy
    10.
    发明申请
    Photobleaching and Intermittency Localization Microscopy 有权
    漂白和间歇定位显微镜

    公开(公告)号：US20120313012A1

    公开(公告)日：2012-12-13

    申请号：US13578218

    申请日：2011-02-22

    申请人： Paul R. Selvin ,  Paul Dennis Simonson

    发明人： Paul R. Selvin ,  Paul Dennis Simonson

    IPC分类号： G01N21/64

    CPC分类号： G01N21/6458 ,  G06T3/4069

    摘要： A method of image analysis creates super-resolution images from images with high densities of fluorophores by processing a movie in which the fluorescent molecules or particles are photobleaching or blinking. The method looks for the individual photobleaching events that can be located with high resolution (nm to tens of nanometers scale). The positions of the photobleaching or blinking events are then drawn in a composite image that is at a much higher resolution than the original fluorescence movie.

    摘要翻译： 图像分析的方法通过处理荧光分子或颗粒光漂白或闪烁的影片，从具有高密度荧光团的图像产生超分辨率图像。 该方法寻找可以以高分辨率（nm到数十纳米级）定位的各个光漂白事件。 然后将光漂白或闪烁事件的位置绘制在比原始荧光电影高得多的分辨率的合成图像中。