公开(公告)号：US07494772B2

公开(公告)日：2009-02-24

申请号：US10630536

申请日：2003-07-29

申请人： Paula M. McCready ,  Lyndsay Radnedge ,  Gary L. Andersen ,  Linda L. Ott ,  Thomas R. Slezak ,  Thomas A. Kuczmarski ,  Vladinir L. Motin

发明人： Paula M. McCready ,  Lyndsay Radnedge ,  Gary L. Andersen ,  Linda L. Ott ,  Thomas R. Slezak ,  Thomas A. Kuczmarski ,  Vladinir L. Motin

IPC分类号： C07H21/02 ,  C07H21/04 ,  C12Q1/68

CPC分类号： C12Q1/689 ,  C12Q1/686

摘要： Nucleotide sequences specific to Yersinia pestis that serve as markers or signatures for identification of this bacterium were identified. In addition, forward and reverse primers and hybridization probes derived from these nucleotide sequences that are used in nucleotide detection methods to detect the presence of the bacterium are disclosed.

摘要翻译： 鉴定了用作鉴定该细菌的标记或特征的鼠疫耶尔森菌特异性的核苷酸序列。 此外，公开了用于检测细菌存在的核苷酸检测方法中使用的来自这些核苷酸序列的正向和反向引物和杂交探针。

公开(公告)号：US07172868B2

公开(公告)日：2007-02-06

申请号：US10630154

申请日：2003-07-29

申请人： Paula M. McCready ,  Lyndsay Radnedge ,  Gary L. Andersen ,  Linda L. Ott ,  Thomas R. Slezak ,  Thomas A. Kuczmarski ,  Elizabeth A Vitalis

发明人： Paula M. McCready ,  Lyndsay Radnedge ,  Gary L. Andersen ,  Linda L. Ott ,  Thomas R. Slezak ,  Thomas A. Kuczmarski ,  Elizabeth A Vitalis

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/689 ,  Y10S435/975

摘要： Described herein is the identification of nucleotide sequences specific to Francisella tularensis that serves as a marker or signature for identification of this bacterium. In addition, forward and reverse primers and hybridization probes derived from these nucleotide sequences that are used in nucleotide detection methods to detect the presence of the bacterium are disclosed.

摘要翻译： 本文描述了用作鉴定该细菌的标记或签名的弗朗西斯氏菌特异性的核苷酸序列的鉴定。 此外，公开了用于检测细菌存在的核苷酸检测方法中使用的来自这些核苷酸序列的正向和反向引物和杂交探针。

公开(公告)号：US07494778B2

公开(公告)日：2009-02-24

申请号：US11644669

申请日：2006-12-21

申请人： Paula M. McCready ,  Lyndsay Radnedge ,  Gary L. Andersen ,  Linda L. Ott ,  Thomas R. Slezak ,  Thomas A. Kuczmarski ,  Elizabeth A Vitalis

发明人： Paula M. McCready ,  Lyndsay Radnedge ,  Gary L. Andersen ,  Linda L. Ott ,  Thomas R. Slezak ,  Thomas A. Kuczmarski ,  Elizabeth A Vitalis

IPC分类号： C07H21/02 ,  C12Q1/68

CPC分类号： C12Q1/689 ,  Y10S435/975

摘要： Described herein is the identification of nucleotide sequences specific to Francisella tularensis that serves as a marker or signature for identification of this bacterium. In addition, forward and reverse primers and hybridization probes derived from these nucleotide sequences that are used in nucleotide detection methods to detect the presence of the bacterium are disclosed.

摘要翻译： 本文描述了用作鉴定该细菌的标记或签名的弗朗西斯氏菌特异性的核苷酸序列的鉴定。 此外，公开了用于检测细菌存在的核苷酸检测方法中使用的来自这些核苷酸序列的正向和反向引物和杂交探针。

公开(公告)号：US07494773B2

公开(公告)日：2009-02-24

申请号：US10630607

申请日：2003-07-29

申请人： Paula M. McCready ,  Lyndsay Radnedge ,  Gary L. Andersen ,  Linda L. Ott ,  Thomas R. Slezak ,  Thomas A. Kuczmarski

发明人： Paula M. McCready ,  Lyndsay Radnedge ,  Gary L. Andersen ,  Linda L. Ott ,  Thomas R. Slezak ,  Thomas A. Kuczmarski

IPC分类号： C07H21/02 ,  C07H21/04 ,  C12Q1/68

CPC分类号： C12Q1/689 ,  C12Q1/686

摘要： Nucleotide sequences specific to Brucella that serves as a marker or signature for identification of this bacterium were identified. In addition, forward and reverse primers and hybridization probes derived from these nucleotide sequences that are used in nucleotide detection methods to detect the presence of the bacterium are disclosed.

摘要翻译： 鉴定了作为用于鉴定该细菌的标记或签名的布鲁杆菌特异性的核苷酸序列。 此外，公开了用于检测细菌存在的核苷酸检测方法中使用的来自这些核苷酸序列的正向和反向引物和杂交探针。

公开(公告)号：US20060040268A1

公开(公告)日：2006-02-23

申请号：US10630154

申请日：2003-07-29

申请人： Paula McCready ,  Lyndsay Radnedge ,  Gary Andersen ,  Linda Ott ,  Thomas Slezak ,  Thomas Kuczmarski ,  Elizabeth Vitalis

发明人： Paula McCready ,  Lyndsay Radnedge ,  Gary Andersen ,  Linda Ott ,  Thomas Slezak ,  Thomas Kuczmarski ,  Elizabeth Vitalis

IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12P19/34

CPC分类号： C12Q1/689 ,  Y10S435/975

摘要： Described herein is the identification of nucleotide sequences specific to Francisella tularensis that serves as a marker or signature for identification of this bacterium. In addition, forward and reverse primers and hybridization probes derived from these nucleotide sequences that are used in nucleotide detection methods to detect the presence of the bacterium are disclosed.

公开(公告)号：US20060019255A1

公开(公告)日：2006-01-26

申请号：US10630607

申请日：2003-07-29

申请人： Paula McCready ,  Lyndsay Radnedge ,  Gary Andersen ,  Linda Ott ,  Thomas Slezak ,  Thomas Kuczmarski

发明人： Paula McCready ,  Lyndsay Radnedge ,  Gary Andersen ,  Linda Ott ,  Thomas Slezak ,  Thomas Kuczmarski

IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12P19/34

CPC分类号： C12Q1/689 ,  C12Q1/686

摘要： Described herein are the identification of nucleotide sequences specific to Brucella that serves as a marker or signature for identification of this bacterium. In addition, forward and reverse primers and hybridization probes derived from these nucleotide sequences that are used in nucleotide detection methods to detect the presence of the bacterium are disclosed.

摘要翻译： 本文描述了作为用于鉴定该细菌的标记或签名的布鲁杆菌特异性的核苷酸序列的鉴定。 此外，公开了用于检测细菌存在的核苷酸检测方法中使用的来自这些核苷酸序列的正向和反向引物和杂交探针。

公开(公告)号：US20060019254A1

公开(公告)日：2006-01-26

申请号：US10630536

申请日：2003-07-29

申请人： Paula McCready ,  Lyndsay Radnedge ,  Gary Andersen ,  Linda Ott ,  Thomas Slezak ,  Thomas Kuczmarski ,  Vladimir Motin

发明人： Paula McCready ,  Lyndsay Radnedge ,  Gary Andersen ,  Linda Ott ,  Thomas Slezak ,  Thomas Kuczmarski ,  Vladimir Motin

IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12P19/34

CPC分类号： C12Q1/689 ,  C12Q1/686

摘要： Described herein are the identification of nucleotide sequences specific to Yersinia pestis that serves as a marker or signature for identification of this bacterium. In addition, forward and reverse primers and hybridization probes derived from these nucleotide sequences that are used in nucleotide detection methods to detect the presence of the bacterium are disclosed.

摘要翻译： 本文描述了用作鉴定该细菌的标记物或签名的鼠疫耶尔森氏菌特异性的核苷酸序列的鉴定。 此外，公开了用于检测细菌存在的核苷酸检测方法中使用的来自这些核苷酸序列的正向和反向引物和杂交探针。

公开(公告)号：US20070111248A1

公开(公告)日：2007-05-17

申请号：US11644669

申请日：2006-12-21

申请人： Paula McCready ,  Lyndsay Radnedge ,  Gary Andersen ,  Linda Ott ,  Thomas Slezak ,  Thomas Kuczmarski ,  Elizabeth Vitalis

发明人： Paula McCready ,  Lyndsay Radnedge ,  Gary Andersen ,  Linda Ott ,  Thomas Slezak ,  Thomas Kuczmarski ,  Elizabeth Vitalis

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/689 ,  Y10S435/975

摘要： Described herein is the identification of nucleotide sequences specific to Francisella tularensis that serves as a marker or signature for identification of this bacterium. In addition, forward and reverse primers and hybridization probes derived from these nucleotide sequences that are used in nucleotide detection methods to detect the presence of the bacterium are disclosed.

公开(公告)号：US20060057603A1

公开(公告)日：2006-03-16

申请号：US11075059

申请日：2005-03-07

申请人： Lyndsay Radnedge ,  Gary Andersen

发明人： Lyndsay Radnedge ,  Gary Andersen

IPC分类号： C12Q1/68

CPC分类号： C12Q1/689 ,  C12Q2600/16

摘要： The present invention utilizes unique DNA signatures to identify Bacillus anthracis. The DNA signatures can also be used in other types of detection. These signatures have been found to be unique to Bacillus anthracis, and can be used to understand how these DNA regions are being used to make this species different from closely related Bacillus species. Mechanisms of virulence, invasion, evolution, interactions with other microbes, antibiotic resistance etc. can be investigated with these regions once they are known. The method comprises using a multiplex polymerase chain reaction analysis of separate loci on the Bacillus anthracis chromosome to produce a set of chromosomal DNA signatures, and using at least one of the chromosomal DNA signatures to detect genetically the Bacillus anthracis.

摘要翻译： 本发明利用独特的DNA特征来鉴定炭疽芽孢杆菌。 DNA签名也可用于其他类型的检测。 已经发现这些特征是炭疽芽孢杆菌是唯一的，并且可以用于了解这些DNA区域如何用于使得该物种与紧密相关的芽孢杆菌属物种不同。 一旦知道毒力，入侵，进化，与其他微生物的相互作用，抗生素耐药性等机制，可以在这些区域进行研究。 该方法包括对炭疽芽孢杆菌染色体上的单独位点进行多重聚合酶链式反应分析以产生一组染色体DNA特征，并使用至少一种染色体DNA标记来遗传地检测炭疽芽孢杆菌。