公开(公告)号：US20050084923A1

公开(公告)日：2005-04-21

申请号：US10489141

申请日：2002-09-13

申请人： Peter-Juergen Mueller ,  Martin Roth ,  Monika Hilliger ,  Stephan Hilliger ,  Ingrid Groth ,  Christel Kummer ,  Karin Martin ,  Volker Schoeckh ,  Jose Metze ,  Michael Kohler ,  Thomas Henkel ,  Gunter Gastrock ,  Karen Lemke

发明人： Peter-Juergen Mueller ,  Martin Roth ,  Monika Hilliger ,  Stephan Hilliger ,  Ingrid Groth ,  Christel Kummer ,  Karin Martin ,  Volker Schoeckh ,  Jose Metze ,  Michael Kohler ,  Thomas Henkel ,  Gunter Gastrock ,  Karen Lemke

IPC分类号： C12N1/00 ,  C12M1/00 ,  C12N1/02 ,  C12Q1/04 ,  C12N1/20

CPC分类号： C12M21/16 ,  C12M41/36

摘要： The invention relates to methods for individually separating microorganisms from a suspension or culture, parallel cultivation of individual cells and analyzing the metabolic performances thereof. The invention enables basic microbial operations such as media optimization, screening according to images of novel natural substances and special metabolic performances, qualitative and quantitative detection of the effects of nutrient substrates, effectors and active substances including the media optimization and selection of microorganism clones with specific properties from large populations according to mutagenesis, transformation, transfection and genetic processing in addition to the detection of microbial contaminations to be carried out. One advantage of the invention is that it can be applied when microorganisms having outstanding properties can be respectively obtained as individual cells or individual organisms from a large population and can be characterized as pure cultures or when the effect of influencing variables can be examined in many fully comparable cultures.

摘要翻译： 本发明涉及用于从悬浮液或培养物中单独分离微生物的方法，并行培养单个细胞并分析其代谢性能。 本发明能够进行基础微生物操作，例如介质优化，根据新型天然物质的图像筛选和特殊代谢性能，定性和定量检测营养底物，作用剂和活性物质的影响，包括介质优化和选择具有特异性的微生物克隆 除了检测要进行的微生物污染外，还要根据诱变，转化，转染和遗传处理从大量种群中获得性状。 本发明的一个优点是可以应用于具有突出性质的微生物作为来自大量群体的单个细胞或个体生物体，并且可以被表征为纯培养物，或者当影响变量的影响可以在许多完全 可比文化。