公开(公告)号：US08986976B2

公开(公告)日：2015-03-24

申请号：US13520685

申请日：2011-01-06

申请人： Phani Kumar Pullela ,  Mulakkapurath Narayanan Manoj ,  Santhosh Kumar Gandhavalla ,  Mitchell Preetham Pinto ,  Chandrasekhar Bhaskaran Nair ,  Pillarisetti Venkata Subbarao

发明人： Phani Kumar Pullela ,  Mulakkapurath Narayanan Manoj ,  Santhosh Kumar Gandhavalla ,  Mitchell Preetham Pinto ,  Chandrasekhar Bhaskaran Nair ,  Pillarisetti Venkata Subbarao

IPC分类号： C12N1/08 ,  C07H1/08 ,  C12N15/10

CPC分类号： C07H1/08 ,  C12N15/10 ,  C12N15/1006

摘要： The present disclosure provides a method to isolate natural & artificial nucleic acids like deoxyribonucleic acid (DNA), ribonucleic acid (RNA) and peptide nucleic acid (PNA) from a solid or liquid sample using cotton. The cotton packed is such that, a solution containing nucleic acids passes through it and the nucleic acids in solution are bound to the cotton in a medium optimal for binding. The nucleic acids are bound to cotton in such a way that, the bound nucleic acids can withstand multiple washes with liquid comprising water and gets eluted in an aqueous buffer, with which eluted nucleic acids can be directly used for amplification using PCR or for any other biochemical or molecular biology needs.

摘要翻译： 本公开提供了使用棉花从固体或液体样品分离天然和人造核酸如脱氧核糖核酸（DNA），核糖核酸（RNA）和肽核酸（PNA）的方法。 包装的棉花使得含有核酸的溶液通过它，并且溶液中的核酸在最适于结合的培养基中与棉花结合。 核酸以这样的方式结合棉花，使得结合的核酸可以经受含有水的液体的多次洗涤，并在水性缓冲液中洗脱，其中洗脱的核酸可以直接用于使用PCR或任何其它的扩增 生物化学或分子生物学的需要。

公开(公告)号：US20130203150A1

公开(公告)日：2013-08-08

申请号：US13520685

申请日：2011-01-06

申请人： Phani Kumar Pullela ,  Mulakkapurath Narayanan Manoj ,  Santhosh Kumar Gandhavalla ,  Mitchell Preetham Pinto ,  Chandrasekhar Bhaskaran Nair ,  Pillarisetti Venkata Subbarao

发明人： Phani Kumar Pullela ,  Mulakkapurath Narayanan Manoj ,  Santhosh Kumar Gandhavalla ,  Mitchell Preetham Pinto ,  Chandrasekhar Bhaskaran Nair ,  Pillarisetti Venkata Subbarao

IPC分类号： C07H1/08

CPC分类号： C07H1/08 ,  C12N15/10 ,  C12N15/1006

摘要： The present disclosure provides a method to isolate natural & artificial nucleic acids like deoxyribonucleic acid (DNA), ribonucleic acid (RNA) and peptide nucleic acid (PNA) from a solid or liquid sample using cotton. The cotton packed is such that, a solution containing nucleic acids passes through it and the nucleic acids in solution are bound to the cotton in a medium optimal for binding. The nucleic acids are bound to cotton in such a way that, the bound nucleic acids can withstand multiple washes with liquid comprising water and gets eluted in an aqueous buffer, with which eluted nucleic acids can be directly used for amplification using PCR or for any other biochemical or molecular biology needs.

摘要翻译： 本公开提供了使用棉花从固体或液体样品分离天然和人造核酸如脱氧核糖核酸（DNA），核糖核酸（RNA）和肽核酸（PNA）的方法。 包装的棉花使得含有核酸的溶液通过它，并且溶液中的核酸在最适于结合的培养基中与棉花结合。 核酸以这样的方式结合棉花，使得结合的核酸可以经受含有水的液体的多次洗涤，并在水性缓冲液中洗脱，其中洗脱的核酸可以直接用于使用PCR或任何其它的扩增 生物化学或分子生物学的需要。