公开(公告)号：US5420013A

公开(公告)日：1995-05-30

申请号：US101259

申请日：1993-08-02

申请人： Philippe Pouletty ,  Peter Chun ,  Chin H. Chang

发明人： Philippe Pouletty ,  Peter Chun ,  Chin H. Chang

IPC分类号： G01N33/569 ,  G01N33/543 ,  G01N33/577

CPC分类号： G01N33/56977 ,  Y10S435/962 ,  Y10S435/967 ,  Y10S435/97 ,  Y10S435/975

摘要： Methods and compositions are provided for detecting HLA polymorphs where distinguishing antibodies are not readily available. Cross-reactive antibodies are employed, where one cross-reacts with the HLA of interest and other alleles, and the other cross-reacts with one of the other alleles, but not with the HLA of interest. By appropriate protocols and controls, the polymorph of interest may be detected.

摘要翻译： 提供了用于检测区分抗体不易获得的HLA多形体的方法和组合物。 使用交叉反应性抗体，其中一个与感兴趣的HLA和其他等位基因交叉反应，另一个与其他等位基因之一交叉反应，但不与感兴趣的HLA交叉反应。 通过适当的方案和对照，可以检测感兴趣的多晶型物。

公开(公告)号：US5256543A

公开(公告)日：1993-10-26

申请号：US698319

申请日：1991-05-10

申请人： Philippe Pouletty ,  Peter Chun

发明人： Philippe Pouletty ,  Peter Chun

IPC分类号： G01N33/53 ,  G01N33/537 ,  G01N33/569 ,  G01N33/543 ,  G01N33/577

CPC分类号： G01N33/56977 ,  Y10S435/962 ,  Y10S435/97 ,  Y10S435/975 ,  Y10S436/809 ,  Y10S436/824 ,  Y10S436/825

摘要： Physiological samples are typed for HLA-B27 by contacting the sample with an antibody which binds to HLA-B7 and is not cross-reactive between HLA-B7 and B27, separating the sample from any complexes which are formed, and then testing the sample for the presence of HLA-B27 with an antibody which is cross-reactive for HLA-B7 and B27 in a STAT test. Particularly, an enzyme conjugate is used which binds to a membrane allowing for the detection of any HLA-B27 bound to the membrane.

摘要翻译： 通过使样品与HLA-B7结合的抗体接触并且在HLA-B7和B27之间不交叉反应，将样品与形成的复合物分离，然后测试样品用于HLA-B27的生理样品 在STAT测试中HLA-B27与HLA-B7和B27交叉反应的抗体的存在。 特别地，使用结合膜的酶缀合物，允许检测与膜结合的任何HLA-B27。

公开(公告)号：US08501495B2

公开(公告)日：2013-08-06

申请号：US12704147

申请日：2010-02-11

申请人： Kai Ling Yao ,  Peter Chun

发明人： Kai Ling Yao ,  Peter Chun

IPC分类号： G01N33/558 ,  G01N33/563 ,  G01N33/53 ,  C12N1/34

CPC分类号： G01N33/54333

摘要： A sequential solid phase immunoassay and system is disclosed. The immunoassay utilizes the secondary antibody method for the detection of antibodies in a membrane-based test. The system comprises a test strip including a nitrocellulose membrane having an immobilized antigen in a capture zone on the membrane and a stabilized liquid secondary antibody conjugate. The sequential solid phase immunoassay is performed in a sequential manner with the addition of a fluid specimen being followed by the addition of the stabilized liquid secondary antibody conjugate. The sequential procedure using the system includes allowing the fluid specimen containing antibodies specific to the antigen to pass laterally from the test strip first end through the capture zone. The immobilized antigens in the capture zone capture antibodies specific to the antigen. The stabilized liquid secondary antibody conjugate then binds to the captured antibodies and can be detected visually or by a machine or reader.

摘要翻译： 公开了连续固相免疫测定和系统。 免疫测定法利用二抗法在基于膜的检测中检测抗体。 该系统包括测试条，其包括在膜上的捕获区中具有固定化抗原的硝酸纤维素膜和稳定的液体二抗缀合物。 顺序的固相免疫测定是按照顺序的方式进行的，随后加入流体样品后加入稳定的液体二抗偶联物。 使用该系统的顺序方法包括允许含有抗原特异性抗体的流体样品从测试条第一端横向通过捕获区。 捕获区域中的固定化抗原捕获抗原特异性的抗体。 稳定的液体二抗缀合物然后结合捕获的抗体，并且可以通过目视检测或通过机器或读取器检测。

公开(公告)号：USD536986S1

公开(公告)日：2007-02-20

申请号：US29210383

申请日：2004-07-30

申请人： Ronald Dale Nix ,  Sam Ciulla ,  Lori Cerwin ,  Scott Hardy ,  Peter Chun ,  Vincent H. Bowman ,  George Nukuto

设计人： Ronald Dale Nix ,  Sam Ciulla ,  Lori Cerwin ,  Scott Hardy ,  Peter Chun ,  Vincent H. Bowman ,  George Nukuto

公开(公告)号：US20100144061A1

公开(公告)日：2010-06-10

申请号：US12704147

申请日：2010-02-11

申请人： Kai Ling Yao ,  Peter Chun

发明人： Kai Ling Yao ,  Peter Chun

IPC分类号： G01N33/543

CPC分类号： G01N33/54333

摘要： A sequential solid phase immunoassay and system is disclosed. The immunoassay utilizes the secondary antibody method for the detection of antibodies in a membrane-based test. The system comprises a test strip including a nitrocellulose membrane having an immobilized antigen in a capture zone on the membrane and a stabilized liquid secondary antibody conjugate. The sequential solid phase immunoassay is performed in a sequential manner with the addition of a fluid specimen being followed by the addition of the stabilized liquid secondary antibody conjugate. The sequential procedure using the system includes allowing the fluid specimen containing antibodies specific to the antigen to pass laterally from the test strip first end through the capture zone. The immobilized antigens in the capture zone capture antibodies specific to the antigen. The stabilized liquid secondary antibody conjugate then binds to the captured antibodies and can be detected visually or by a machine or reader.

摘要翻译： 公开了连续固相免疫测定和系统。 免疫测定法利用二抗法在基于膜的检测中检测抗体。 该系统包括测试条，其包括在膜上的捕获区中具有固定化抗原的硝酸纤维素膜和稳定的液体二抗缀合物。 顺序的固相免疫测定是按照顺序的方式进行的，随后加入流体样品后加入稳定的液体二抗偶联物。 使用该系统的顺序方法包括允许含有抗原特异性抗体的流体样品从测试条第一端横向通过捕获区。 捕获区域中的固定化抗原捕获抗原特异性的抗体。 稳定的液体二抗缀合物然后结合捕获的抗体，并且可以通过目视检测或通过机器或读取器检测。

公开(公告)号：USD536976S1

公开(公告)日：2007-02-20

申请号：US29234583

申请日：2005-07-20

申请人： Sam Ciulla ,  Lori Cerwin ,  Peter Chun ,  Vincent H. Bowman

设计人： Sam Ciulla ,  Lori Cerwin ,  Peter Chun ,  Vincent H. Bowman

公开(公告)号：US06245721B1

公开(公告)日：2001-06-12

申请号：US09432875

申请日：1999-11-02

申请人： Peter Chun ,  John A. Elverum

发明人： Peter Chun ,  John A. Elverum

IPC分类号： C10M13114

CPC分类号： C10M171/00

摘要： The lubricant additive formulation for introduction into a petroleum based carrier, such as, motor oil, in which the additive contains substantially equal amounts of naphthenic oil and chlorinated paraffin with minor amounts of a paraffin oil and anti-wear and anti-oxidant agents; and in certain applications, metal powders or soaps are introduced into the additive together with an effective amount of grease necessary to maintain the metal particles in suspension when introduced into the liquid carrier.

摘要翻译： 用于引入石油基载体例如机油的润滑添加剂配方，其中添加剂含有基本上相等量的环烷油和氯化石蜡与少量的石蜡油和抗磨损和抗氧化剂; 并且在某些应用中，将金属粉末或肥皂与引入液体载体中的金属颗粒保持悬浮液所需的有效量的润滑脂一起引入添加剂中。