公开(公告)号：US20240002808A1

公开(公告)日：2024-01-04

申请号：US18226184

申请日：2023-07-25

Applicant: President and Fellows of Harvard College

Inventor： Richard Novak ,  Sasan Jalili-Firoozinezhad ,  Francesca S. Gazzaniga ,  Elizabeth Calamari ,  Diogo M. Camacho ,  Bret Andrew Nestor ,  Cicely Fadel ,  Michael J. Cronce ,  Dennis Kasper ,  Donald E. Ingber ,  Amir Bein

IPC: C12N5/071 ,  C12M3/00 ,  C12M3/06 ,  C12N1/20

CPC classification number: C12N5/0679 ,  C12N1/20 ,  C12M23/16 ,  C12M21/08

Abstract: A microfluidic device is directed to sustaining a complex microbial community in direct and indirect contact with living human intestinal cells in vitro. The device includes a first microchannel having cultured cells of a human intestinal epithelium and microbiota, the first microchannel further having a first level of oxygen. The device further includes a second microchannel having cultured cells of a vascular endothelium, the second microchannel further having a second level of oxygen. The device also includes a membrane located at an interface region between the first microchannel and the second microchannel, the membrane being composed of an oxygen-permeable material or further having pores via which oxygen flows between the first microchannel and the second microchannel to form a physiologically-relevant oxygen gradient.