公开(公告)号：US12187996B2

公开(公告)日：2025-01-07

申请号：US18056035

申请日：2022-11-16

Applicant: President and Fellows of Harvard College

Inventor： Antonio Varone ,  Norman Wen ,  Daniel Levner ,  Richard Novak ,  Lori McPartlin ,  Donald E. Ingber ,  Youngjae Choe ,  Lian Leng ,  Justin K. Nguyen

IPC: C12M3/06 ,  B01L3/00 ,  C12M1/00 ,  C12M1/12 ,  C12M1/34 ,  C12M3/00 ,  C12M3/04 ,  C12N5/071 ,  C12N5/077

Abstract: A device for simulating a function of a tissue includes a first structure, a second structure, and a membrane. The first structure defines a first chamber. The first chamber includes a matrix disposed therein and an opened region. The second structure defines a second chamber. The membrane is located at an interface region between the first chamber and the second chamber. The membrane includes a first side facing toward the first chamber and a second side facing toward the second chamber. The membrane separates the first chamber from the second chamber.

公开(公告)号：US12180447B2

公开(公告)日：2024-12-31

申请号：US18343514

申请日：2023-06-28

Applicant: President and Fellows of Harvard College ,  The General Hospital Corporation

Inventor： David Benson Chou ,  Liliana S. Teixeira Moreira Leijten ,  Arianna Rech ,  Richard Novak ,  Donald E. Ingber ,  Yuka Milton ,  Viktoras Frismantas ,  Oren Levy

IPC: C12M3/06 ,  B01L3/00 ,  C12M1/00 ,  C12M1/12 ,  C12N5/077 ,  G01N33/50

Abstract: The present disclosure relates to a microfluidic devices and methods for culturing bone marrow cells. Aspects include methods of preparing microfluidic devices and culturing bone marrow cells with the microfluidic devices. In some aspects, a method includes providing a microfluidic device having an upper chamber, a lower chamber, and a porous membrane separating the upper chamber from the lower chamber. The method further includes seeding walls of the lower chamber and a bottom surface of the membrane with endothelial cells. The method further includes providing a matrix within the upper chamber. The matrix includes fibrin gel and bone marrow cells. The method further includes filling or perfusing the upper chamber with a media.

公开(公告)号：US12104174B2

公开(公告)日：2024-10-01

申请号：US16331718

申请日：2017-09-13

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Donald E. Ingber ,  Magdalena Kasendra ,  Alexandra Sontheimer-Phelps ,  Alessio Tovaglieri

IPC: C12N5/071 ,  C12N5/00 ,  G01N33/50

CPC classification number: C12N5/0697 ,  C12N5/0062 ,  C12N5/0068 ,  C12N5/0679 ,  G01N33/5088 ,  C12N2501/11 ,  C12N2501/345 ,  C12N2501/415 ,  C12N2502/1323

Abstract: Described herein are methods for providing an in vitro intestinal model system, e.g., using primary cells instead of cell lines and/or cancerous cells.

公开(公告)号：US20240218309A1

公开(公告)日：2024-07-04

申请号：US18604469

申请日：2024-03-13

Applicant: President and Fellows of Harvard College

Inventor： Antonio Varone ,  Norman Wen ,  Daniel Levner ,  Richard Novak ,  Lori McPartlin ,  Donald E. Ingber ,  Youngjae Choe ,  Lian Leng ,  Justin K. Nguyen

IPC: C12M3/06 ,  C12M1/12 ,  C12M3/00 ,  C12N5/071 ,  C12N5/077 ,  B01L3/00 ,  C12M1/00 ,  C12M1/34 ,  C12M3/04

CPC classification number: C12M23/16 ,  C12M21/08 ,  C12M25/02 ,  C12M25/14 ,  C12N5/0629 ,  C12N5/0656 ,  C12N5/0679 ,  C12N5/069 ,  B01L3/5027 ,  B01L2300/069 ,  C12M3/04 ,  C12M29/10 ,  C12M41/46

Abstract: A device for simulating a function of a tissue includes a first structure, a second structure, and a membrane. The first structure defines a first chamber. The first chamber includes a matrix disposed therein and an opened region. The second structure defines a second chamber. The membrane is located at an interface region between the first chamber and the second chamber. The membrane includes a first side facing toward the first chamber and a second side facing toward the second chamber. The membrane separates the first chamber from the second chamber.

公开(公告)号：US11976304B2

公开(公告)日：2024-05-07

申请号：US17539452

申请日：2021-12-01

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Donald E. Ingber ,  Magdalena Kasendra ,  Alexandra Sontheimer-Phelps ,  Alessio Tovaglieri

IPC: C12N5/071 ,  C12N5/00 ,  G01N33/50

CPC classification number: C12N5/0697 ,  C12N5/0062 ,  C12N5/0068 ,  C12N5/0679 ,  G01N33/5088 ,  C12N2501/11 ,  C12N2501/345 ,  C12N2501/415 ,  C12N2502/1323

Abstract: Described herein are methods for providing an in vitro intestinal model system, e.g., using primary cells instead of cell lines and/or cancerous cells.

公开(公告)号：US20230357690A1

公开(公告)日：2023-11-09

申请号：US18343514

申请日：2023-06-28

Applicant: President and Fellows of Harvard College ,  The General Hospital Corporation

Inventor： David Benson Chou ,  Liliana S. Teixeira Moreira Leijten ,  Arianna Rech ,  Richard Novak ,  Donald E. Ingber ,  Yuka Milton ,  Viktoras Frismantas ,  Oren Levy

IPC: C12M3/06 ,  B01L3/00 ,  C12M1/12 ,  C12M1/00 ,  C12N5/077 ,  G01N33/50

CPC classification number: C12M23/16 ,  B01L3/502715 ,  C12M25/02 ,  C12M29/10 ,  C12N5/0669 ,  G01N33/5008 ,  B01L2300/12 ,  C12N2501/40 ,  C12N2501/999 ,  C12N2502/28 ,  C12N2533/54 ,  C12N2533/56 ,  C12N2533/90

Abstract: The present disclosure relates to a microfluidic devices and methods for culturing bone marrow cells. Aspects include methods of preparing microfluidic devices and culturing bone marrow cells with the microfluidic devices. In some aspects, a method includes providing a microfluidic device having an upper chamber, a lower chamber, and a porous membrane separating the upper chamber from the lower chamber. The method further includes seeding walls of the lower chamber and a bottom surface of the membrane with endothelial cells. The method further includes providing a matrix within the upper chamber. The matrix includes fibrin gel and bone marrow cells. The method further includes filling or perfusing the upper chamber with a media.

公开(公告)号：US11773359B2

公开(公告)日：2023-10-03

申请号：US17146264

申请日：2021-01-11

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Donald E. Ingber ,  Anthony Bahinski ,  Robert Cunningham ,  Josue A. Goss ,  Geraldine A. Hamilton ,  Christopher David Hinojosa ,  Daniel Levner ,  Kevin Kit Parker

IPC: C12M3/06 ,  C12M1/34 ,  G01N33/50 ,  B01L3/00 ,  B01L9/00 ,  C12M3/00 ,  C12M1/00 ,  C12M1/42 ,  A01N1/02

CPC classification number: C12M23/16 ,  A01N1/02 ,  B01L3/502715 ,  B01L9/52 ,  C12M21/08 ,  C12M23/42 ,  C12M29/10 ,  C12M35/04 ,  C12M35/08 ,  C12M41/00 ,  G01N33/5082 ,  G01N33/5088 ,  A01N1/021 ,  A01N1/0247 ,  A01N1/0273 ,  B01L3/5027 ,  B01L2200/0684 ,  B01L2200/0689 ,  B01L2200/146 ,  B01L2200/147 ,  B01L2300/044 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0663 ,  B01L2300/0867 ,  B01L2300/0877 ,  B01L2300/123 ,  B01L2300/18 ,  B01L2400/0487 ,  B01L2400/0622 ,  B01L2400/0644 ,  C12M41/12 ,  C12M41/46 ,  G01N33/5005

Abstract: The invention provides integrated Organ-on-Chip microphysiological systems representations of living Organs and support structures for such microphysiological systems.

公开(公告)号：US11638748B2

公开(公告)日：2023-05-02

申请号：US17015177

申请日：2020-09-09

Applicant: President and Fellows of Harvard College

Inventor： Michael Super ,  Edward J. Doherty ,  Mark Joseph Cartwright ,  Des White ,  Alexander Stafford ,  Omar Abdel-Rahman Ali ,  Amanda Graveline ,  Donald E. Ingber ,  David J. Mooney ,  Benjamin Seiler

IPC: A61K39/02 ,  A61K39/108 ,  A61K39/00

Abstract: The present invention provides vaccine compositions and methods of producing such compositions. Other embodiments of the invention include methods of treating a pathogen infection, methods of vaccinating a subject against a pathogen infection, and methods for treating an antibiotic-resistance bacterial infection in a subject in need thereof. In further embodiments, the invention includes methods of decreasing the level of a pathogen in a subject having a pathogen infection, methods of increasing the surviving rate of a subject having a pathogen infection, methods of reducing the level of pain associated with a pathogen infection, and methods of reducing the level of distress associated with a pathogen infection in a subject in need thereof. Novel scaffold compositions and opsonin-bound or lectin-bound pathogen compositions, and uses thereof, are also provided herein.

公开(公告)号：US11505773B2

公开(公告)日：2022-11-22

申请号：US15781078

申请日：2016-12-02

Applicant: President and Fellows of Harvard College

Inventor： Antonio Varone ,  Norman Wen ,  Daniel Levner ,  Richard Novak ,  Lori McPartlin ,  Donald E. Ingber ,  Youngjae Choe ,  Lian Leng ,  Justin K. Nguyen

IPC: C12M3/00 ,  B01L3/00 ,  C12M1/00 ,  C12M3/06 ,  C12M1/12 ,  C12N5/071 ,  C12N5/077 ,  C12M3/04 ,  C12M1/34

Abstract: A device for simulating a function of a tissue includes a first structure, a second structure, and a membrane. The first structure defines a first chamber. The first chamber includes a matrix disposed therein and an opened region. The second structure defines a second chamber. The membrane is located at an interface region between the first chamber and the second chamber. The membrane includes a first side facing toward the first chamber and a second side facing toward the second chamber. The membrane separates the first chamber from the second chamber.

公开(公告)号：US20220202929A1

公开(公告)日：2022-06-30

申请号：US17613011

申请日：2020-05-19

Applicant: President and Fellows of Harvard College

Inventor： Donald E. Ingber ,  Longlong Si ,  Rachelle Prantil-Baun

IPC: A61K39/145 ,  C12N7/00 ,  A61P31/16

Abstract: This disclosure provides immunogenic compositions and methods of producing immunogenic compositions sufficient to produce an antigen-specific immune response against variant influenza virus strains. Also provided herein are methods of identifying drug-resistant influenza virus strains.