公开(公告)号：US20200292482A1

公开(公告)日：2020-09-17

申请号：US16760723

申请日：2018-10-30

Applicant: President and Fellows of Harvard College

Inventor： Donhee Ham ,  Hongkun Park ,  Keith Krenek ,  Tianyang Ye ,  Jeffrey T. Abbott ,  Wenxuan Wu

IPC: G01N27/327 ,  C12M1/34 ,  C12N13/00 ,  G01N27/416

Abstract: Methods and systems for monitoring the activity of electrogenic networks are described. One representative system includes an array of electrode coupled to an analyzer having a stimulator and a receiver. The electrode is placed in contact with an electrogenic cell. The electrodes can be shaped as nanowires, tubes, cavities and/or cones. The analyzer may be configured to operate in a voltage stimulation mode, in which the cells are stimulated via voltages and monitored via current, or in a current stimulation mode, in which the cells are stimulated via currents and monitored via voltages. The analyzers may be arranged as single-stage amplifiers, and may include a feedback loop shared between the stimulation signal path and the sensing signal path. The feedback loop may be arranged to provide overlapping stimulation and sensing of the electrogenic network's cells.

公开(公告)号：US12174140B2

公开(公告)日：2024-12-24

申请号：US16760723

申请日：2018-10-30

Applicant: President and Fellows of Harvard College

Inventor： Donhee Ham ,  Hongkun Park ,  Keith Krenek ,  Tianyang Ye ,  Jeffrey T. Abbott ,  Wenxuan Wu

IPC: G01N27/327 ,  C12M1/34 ,  C12N13/00 ,  G01N27/416

Abstract: Methods and systems for monitoring the activity of electrogenic networks are described. One representative system includes an array of electrode coupled to an analyzer having a stimulator and a receiver. The electrode is placed in contact with an electrogenic cell. The electrodes can be shaped as nanowires, tubes, cavities and/or cones. The analyzer may be configured to operate in a voltage stimulation mode, in which the cells are stimulated via voltages and monitored via current, or in a current stimulation mode, in which the cells are stimulated via currents and monitored via voltages. The analyzers may be arranged as single-stage amplifiers, and may include a feedback loop shared between the stimulation signal path and the sensing signal path. The feedback loop may be arranged to provide overlapping stimulation and sensing of the electrogenic network's cells.