公开(公告)号：US12174140B2

公开(公告)日：2024-12-24

申请号：US16760723

申请日：2018-10-30

Applicant: President and Fellows of Harvard College

Inventor： Donhee Ham ,  Hongkun Park ,  Keith Krenek ,  Tianyang Ye ,  Jeffrey T. Abbott ,  Wenxuan Wu

IPC: G01N27/327 ,  C12M1/34 ,  C12N13/00 ,  G01N27/416

Abstract: Methods and systems for monitoring the activity of electrogenic networks are described. One representative system includes an array of electrode coupled to an analyzer having a stimulator and a receiver. The electrode is placed in contact with an electrogenic cell. The electrodes can be shaped as nanowires, tubes, cavities and/or cones. The analyzer may be configured to operate in a voltage stimulation mode, in which the cells are stimulated via voltages and monitored via current, or in a current stimulation mode, in which the cells are stimulated via currents and monitored via voltages. The analyzers may be arranged as single-stage amplifiers, and may include a feedback loop shared between the stimulation signal path and the sensing signal path. The feedback loop may be arranged to provide overlapping stimulation and sensing of the electrogenic network's cells.

公开(公告)号：US11774396B2

公开(公告)日：2023-10-03

申请号：US17940174

申请日：2022-09-08

Applicant: President and Fellows of Harvard College

Inventor： Hongkun Park ,  Jeffrey T. Abbott ,  Wenxuan Wu ,  Tianyang Ye ,  Han Sae Jung ,  Donhee Ham

IPC: G01N27/327 ,  B01J19/08 ,  C12Q1/02 ,  G01N33/483

CPC classification number: G01N27/3277 ,  B01J19/087 ,  C12Q1/02 ,  G01N33/4836

Abstract: Disclosed herein are an apparatus for electrically assessing and/or manipulating cells. One aspect is directed to electrically mapping cells on the surface of the semiconductor substrate via cross-electrode impedance measurements. Further according to some aspects, the electrode array allows for spatially addressable electrical stimulation and/or recording of electrical signals in real-time using the CMOS circuitry. Some of these aspects are directed to using an electrode array to perform cell patterning through electrochemical gas generation, and extracellular electrochemical mapping.

公开(公告)号：US11366296B2

公开(公告)日：2022-06-21

申请号：US15778208

申请日：2016-11-23

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Robert C. Devlin ,  Mohammadreza Khorasaninejad ,  Federico Capasso ,  Hongkun Park ,  Alexander Arthur High

IPC: G02B1/02 ,  G02B13/14 ,  G02B1/00 ,  H01L31/02 ,  G03F7/00 ,  G03F7/40 ,  C23C16/56 ,  C23C16/455 ,  C23C16/04

Abstract: A method of fabricating a visible spectrum optical component includes: providing a substrate; forming a resist layer over a surface of the substrate; patterning the resist layer to form a patterned resist layer defining openings exposing portions of the surface of the substrate; performing deposition to form a dielectric film over the patterned resist layer and over the exposed portions of the surface of the substrate, wherein a top surface of the dielectric film is above a top surface of the patterned resist layer; removing a top portion of the dielectric film to expose the top surface of the patterned resist layer and top surfaces of dielectric units within the openings of the patterned resist layer; and removing the patterned resist layer to retain the dielectric units over the substrate.

公开(公告)号：US20210187280A1

公开(公告)日：2021-06-24

申请号：US17144387

申请日：2021-01-08

Applicant: President and Fellows of Harvard College

Inventor： Hongkun Park ,  Donhee Ham ,  Jeffrey T. Abbott ,  Ling Qin ,  Marsela Jorgolli ,  Tianyang Ye

IPC: A61N1/05 ,  H01L23/48 ,  H01L29/06 ,  A61N1/06 ,  B82Y5/00 ,  H01L23/49

Abstract: The present invention generally relates to nanowires. In one aspect, the present invention is generally directed to systems and methods of individually addressing nanowires on a surface, e.g., that are substantially upstanding or vertically-oriented with respect to the surface. In some cases, one or more nanowires may be individually addressed using various integrated circuit (“IC”) technologies, such as CMOS. For example, the nanowires may form an array on top of an active CMOs integrated circuit.

公开(公告)号：US20200081017A1

公开(公告)日：2020-03-12

申请号：US16675398

申请日：2019-11-06

Applicant: The Brigham and Women's Hospital, Inc. ,  The Broad Institute, Inc. ,  Massachusetts Institute of Technology ,  President and Fellows of Harvard College

Inventor： Vijay K. Kuchroo ,  Aviv Regev ,  Jellert Gaublomme ,  Youjin Lee ,  Alexander K. Shalek ,  Chao Wang ,  Nir Yosef ,  Hongkun Park

IPC: G01N33/68 ,  C12Q1/6883 ,  C07K16/28

Abstract: This invention relates generally to compositions and methods for identifying the regulatory network that modulates, controls or otherwise influences T cell balance, for example, Th17 cell differentiation, maintenance and/or function, as well compositions and methods for exploiting the regulatory network that modulates, controls or otherwise influences T cell balance in a variety of therapeutic and/or diagnostic indications. This invention also relates generally to identifying and exploiting target genes and/or target gene products that modulate, control or otherwise influence T cell balance in a variety of therapeutic and/or diagnostic indications.

公开(公告)号：US09518336B2

公开(公告)日：2016-12-13

申请号：US14646384

申请日：2013-12-04

Applicant: ELEMENT SIX LIMITED ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Matthew Markham ,  Alastair Stacey ,  Nathalie De Leon ,  Yiwen Chu ,  Brendan John Shields ,  Birgit Judith Maria Hausmann ,  Patrick Maletinsky ,  Ruffin Eley Evans ,  Amir Yacoby ,  Hongkun Park ,  Marko Loncar ,  Mikhail D. Lukin

IPC: C01B31/06 ,  C30B1/04 ,  C30B29/04 ,  C30B33/02 ,  G06N99/00 ,  B82Y10/00 ,  C30B30/00 ,  G01N21/64

CPC classification number: C30B1/04 ,  B82Y10/00 ,  C30B29/04 ,  C30B30/00 ,  C30B33/02 ,  G01N21/64 ,  G01N2021/6497 ,  G06N99/002

Abstract: A synthetic diamond material comprising one or more spin defects having a full width half maximum intrinsic inhomogeneous zero phonon line width of no more than 100 MHz. The method for obtain such a material involves a multi-stage annealing process.

Abstract translation: 一种合成金刚石材料，其包含一个或多个具有不超过100MHz的全宽半最大固有不均匀零声子线宽的自旋缺陷。 获得这种材料的方法包括多阶段退火工艺。

公开(公告)号：US20240325731A1

公开(公告)日：2024-10-03

申请号：US18501690

申请日：2023-11-03

Applicant: President and Fellows of Harvard College

Inventor： Hongkun Park ,  Donhee Ham ,  Jeffrey T. Abbott ,  Ling Qin ,  Marsela Jorgolli ,  Tianyang Ye

IPC: A61N1/05 ,  A61N1/06 ,  B82Y5/00 ,  H01L23/48 ,  H01L23/49 ,  H01L29/06

CPC classification number: A61N1/0531 ,  A61N1/05 ,  A61N1/0536 ,  A61N1/06 ,  B82Y5/00 ,  H01L23/48 ,  H01L23/49 ,  H01L29/0676 ,  H01L2924/0002 ,  H01L2924/10253 ,  H01L2924/14

Abstract: The present invention generally relates to nanowires. In one aspect, the present invention is generally directed to systems and methods of individually addressing nanowires on a surface, e.g., that are substantially upstanding or vertically-oriented with respect to the surface. In some cases, one or more nanowires may be individually addressed using various integrated circuit (“IC”) technologies, such as CMOS. For example, the nanowires may form an array on top of an active CMOs integrated circuit.

公开(公告)号：US20240219370A1

公开(公告)日：2024-07-04

申请号：US18365172

申请日：2023-08-03

Applicant: President and Fellows of Harvard College

Inventor： Donhee Ham ,  Jeffrey T. Abbott ,  Hongkun Park ,  Wenxuan Wu

IPC: G01N33/487 ,  A61B5/053 ,  A61B5/24 ,  A61N1/18 ,  B82Y15/00 ,  C12M1/42 ,  C12N13/00 ,  G01N27/00

CPC classification number: G01N33/48728 ,  A61B5/053 ,  A61B5/24 ,  A61N1/18 ,  C12M35/02 ,  C12N13/00 ,  G01N27/00 ,  A61B2562/0285 ,  B82Y15/00

Abstract: Methods and systems for stimulating and monitoring electrogenic cells are described. Some systems for stimulating electrogenic cells are based on the injection of electric currents into the cells via electrodes connected to the cells. Such stimulators may comprise an impedance element having an input terminal and an output terminal coupled to an electrode, and a voltage follower coupled between the input terminal and the output terminal of the impedance element, the voltage follower being configured to maintain a substantially constant voltage between the input terminal and the output terminal of the impedance element. The impedance element may comprise one or more switched capacitors at least in some embodiments. In some embodiments, the voltage follower may be implemented using a source follower.

公开(公告)号：US11815668B2

公开(公告)日：2023-11-14

申请号：US17824823

申请日：2022-05-25

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Robert C. Devlin ,  Mohammadreza Khorasaninejad ,  Federico Capasso ,  Hongkun Park ,  Alexander Arthur High

IPC: G02B1/00 ,  G02B1/02 ,  G02B13/14 ,  H01L31/02 ,  G03F7/00 ,  G03F7/40 ,  C23C16/56 ,  C23C16/455 ,  C23C16/04

CPC classification number: G02B13/14 ,  C23C16/042 ,  C23C16/45525 ,  C23C16/45555 ,  C23C16/56 ,  G02B1/002 ,  G02B1/005 ,  G03F7/0005 ,  G03F7/40 ,  H01L31/02 ,  G02B1/02

Abstract: A method of fabricating a visible spectrum optical component includes: providing a substrate; forming a resist layer over a surface of the substrate; patterning the resist layer to form a patterned resist layer defining openings exposing portions of the surface of the substrate; performing deposition to form a dielectric film over the patterned resist layer and over the exposed portions of the surface of the substrate, wherein a top surface of the dielectric film is above a top surface of the patterned resist layer; removing a top portion of the dielectric film to expose the top surface of the patterned resist layer and top surfaces of dielectric units within the openings of the patterned resist layer; and removing the patterned resist layer to retain the dielectric units over the substrate.

公开(公告)号：US20230056865A1

公开(公告)日：2023-02-23

申请号：US17869940

申请日：2022-07-21

Applicant: SAMSUNG ELECTRONICS CO., LTD. ,  President and Fellows of Harvard College

Inventor： Seijoon Kim ,  Renjing Xu ,  Hongkun Park ,  Donhee Ham ,  SANG JOON KIM

IPC: G06N3/06

Abstract: A method and apparatus with artificial network generation and/or implementation corresponding to a natural neural network are disclosed. The method includes performing an adjusting, based on a firing time difference between an action potential (AP) of a first biological neuron and a post-synaptic potential (PSP) of a second biological neuron, of a first conductance value of a first memory corresponding to the first and second biological neurons, adjusting the firing time difference based on the PSP of the second biological neuron, and performing another adjusting, by controlling another firing time difference between the AP of the first biological neuron and the PSP of the second biological neuron to be the adjusted firing time difference, of a second conductance value of the first memory corresponding to the first and second biological neurons.