公开(公告)号：US11208652B2

公开(公告)日：2021-12-28

申请号：US16075247

申请日：2017-02-02

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Luhan Yang ,  Margo R. Monroe

IPC: C12N15/113 ,  C12N9/22 ,  C12N15/10 ,  C12N15/63 ,  C12N7/00 ,  A61K48/00

Abstract: Methods and compositions of altering mitochondrial DNA of a eukaryotic cell are provided using one or more of a mitochondrial specific adeno-associated virus to deliver one or more nucleic acids encoding CRISPR system including a Cas9 protein or its nuclease inactive variant and a guide RNA into a mitochondria for expression within the mitochondria. The Cas9 system can cut, nick or regulate a target mitochondrial nucleic acid.

公开(公告)号：US20190062739A1

公开(公告)日：2019-02-28

申请号：US16075247

申请日：2017-02-02

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Luhan Yang ,  Margo R. Monroe

IPC: C12N15/113 ,  C12N9/22 ,  C12N7/00

Abstract: Methods and compositions of altering mitochondrial DNA of a eukaryotic cell are provided using one or more of a mitochondrial specific adeno-associated virus to deliver one or more nucleic acids encoding CRISPR system including a Cas9 protein or its nuclease inactive variant and a guide RNA into a mitochondria for expression within the mitochondria. The Cas9 system can cut, nick or regulate a target mitochondrial nucleic acid.

公开(公告)号：US11746349B2

公开(公告)日：2023-09-05

申请号：US16076022

申请日：2017-02-07

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Luhan Yang ,  Margo R. Monroe ,  Po-Yi Huang

IPC: C12N15/113 ,  C12N9/22 ,  C12N9/96 ,  C12N1/15

CPC classification number: C12N15/113 ,  C12N9/22 ,  C12N9/96 ,  C12Y306/04013

Abstract: Methods and compositions of altering a eukaryotic cell are described including providing to the eukaryotic cell a guide DNA sequence complementary to a target nucleic acid sequence, providing to the eukaryotic cell an Ago enzyme or a nuclease null Ago protein that interacts with the guide DNA sequence for DNA-guided gene editing and regulation of the target nucleic acid sequence in a site specific manner.

公开(公告)号：US20210189388A1

公开(公告)日：2021-06-24

申请号：US16076022

申请日：2017-02-07

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Luhan Yang ,  Margo R. Monroe ,  Po-Yi Huang

IPC: C12N15/113 ,  C12N9/22

Abstract: Methods and compositions of altering a eukaryotic cell are described including providing to the eukaryotic cell a guide DNA sequence complementary to a target nucleic acid sequence, providing to the eukaryotic cell an Ago enzyme or a nuclease null Ago protein that interacts with the guide DNA sequence for DNA-guided gene editing and regulation of the target nucleic acid sequence in a site specific manner.