公开(公告)号：US11512325B2

公开(公告)日：2022-11-29

申请号：US17672744

申请日：2022-02-16

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Prashant G. Mali ,  Luhan Yang

IPC: C12N15/00 ,  C12N15/85 ,  C12N15/10 ,  C12N15/63 ,  C12N15/90 ,  C12N15/01 ,  C12N15/81 ,  C12N15/82 ,  C12N15/87 ,  C12N9/22

Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.

公开(公告)号：US10925263B2

公开(公告)日：2021-02-23

申请号：US16708655

申请日：2019-12-10

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Luhan Yang ,  Marc Guell

IPC: A01N63/00 ,  C12N15/63 ,  C12N15/64 ,  A01K67/027 ,  C12N15/113 ,  C12N15/85 ,  C12N7/00 ,  C12N9/22 ,  C12N15/11 ,  C12N15/90

Abstract: A method of modulating some or all copies of a gene in a cell is provided including introducing into a cell one or more ribonucleic acid (RNA) sequences that comprise a portion that is complementary to all or a portion of each of the one or more target nucleic acid sequences, and a nucleic acid sequence that encodes a Cas protein and maintaining the cells under conditions in which the Cas protein is expressed and the Cas protein binds and modulates the one or more target nucleic acid sequences in the cell.

公开(公告)号：US20200308599A1

公开(公告)日：2020-10-01

申请号：US16884327

申请日：2020-05-27

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Prashant G. Mali ,  Luhan Yang

IPC: C12N15/85 ,  C12N15/10 ,  C12N15/63 ,  C12N15/90 ,  C12N15/01 ,  C12N15/81 ,  C12N15/82 ,  C12N15/87 ,  C12N9/22

Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.

公开(公告)号：US10375938B2

公开(公告)日：2019-08-13

申请号：US15888581

申请日：2018-02-05

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Luhan Yang ,  Marc Guell

IPC: A01K67/027 ,  C12N15/63 ,  C12N15/113 ,  C12N7/00 ,  C12N9/22 ,  C12N15/11 ,  C12N15/90

Abstract: A method of modulating some or all copies of a gene in a cell is provided including introducing into a cell one or more ribonucleic acid (RNA) sequences that comprise a portion that is complementary to all or a portion of each of the one or more target nucleic acid sequences, and a nucleic acid sequence that encodes a Cas protein and maintaining the cells under conditions in which the Cas protein is expressed and the Cas protein binds and modulates the one or more target nucleic acid sequences in the cell.

公开(公告)号：US20160369301A1

公开(公告)日：2016-12-22

申请号：US14907605

申请日：2014-07-25

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： George M. Church ,  Luhan Yang ,  Marc Guell ,  Joyce Lichi Yang

IPC: C12N15/90 ,  C12N5/074 ,  C12N15/86

CPC classification number: C12N15/907 ,  C12N5/0696 ,  C12N15/85 ,  C12N15/86 ,  C12N2510/00 ,  C12N2740/15043 ,  C12N2800/90 ,  C12N2830/003

Abstract: Methods of genome engineering in cells using a TALEN lacking repeat sequences or Cas9 is provided.

公开(公告)号：US20150259704A1

公开(公告)日：2015-09-17

申请号：US14681510

申请日：2015-04-08

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Prashant Mali ,  Luhan Yang

IPC: C12N15/85

Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.

Abstract translation: 提供了改变真核细胞的方法，包括用编码与真核细胞的基因组DNA互补的RNA的核酸转染真核细胞，用编码与RNA相互作用并切割基因组DNA的酶的核酸转染真核细胞 以位点特异性方式，其中细胞表达RNA和酶，RNA结合互补基因组DNA，酶以位点特异性方式切割基因组DNA。

公开(公告)号：US20140342457A1

公开(公告)日：2014-11-20

申请号：US14319171

申请日：2014-06-30

Applicant: President and Fellows of Harvard College

Inventor： Prashant G. MALI ,  George M. CHURCH ,  Luhan Yang

IPC: C12N15/01 ,  C12N15/82 ,  C12N15/85 ,  C12N15/81

Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.

公开(公告)号：US20230295653A1

公开(公告)日：2023-09-21

申请号：US18296579

申请日：2023-04-06

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Prashant G. Mali ,  Luhan Yang

IPC: C12N15/85 ,  C12N15/10 ,  C12N15/63 ,  C12N15/90 ,  C12N15/01 ,  C12N15/81 ,  C12N15/82 ,  C12N15/87 ,  C12N9/22

CPC classification number: C12N15/85 ,  C12N15/10 ,  C12N15/102 ,  C12N15/63 ,  C12N15/90 ,  C12N15/01 ,  C12N15/81 ,  C12N15/8201 ,  C12N15/87 ,  C12N15/907 ,  C12N15/1024 ,  C12N9/22 ,  C12N2310/20 ,  C12N2810/55 ,  C12Y301/00 ,  C12N2800/80

Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.

公开(公告)号：US11064684B2

公开(公告)日：2021-07-20

申请号：US16531351

申请日：2019-08-05

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Luhan Yang ,  Marc Guell

IPC: A01N63/00 ,  C12N15/63 ,  C12N15/64 ,  A01K67/027 ,  C12N15/113 ,  C12N15/85 ,  C12N7/00 ,  C12N9/22 ,  C12N15/11 ,  C12N15/90 ,  C07H21/04 ,  C07H21/02

Abstract: A method of modulating some or all copies of a gene in a cell is provided including introducing into a cell one or more ribonucleic acid (RNA) sequences that comprise a portion that is complementary to all or a portion of each of the one or more target nucleic acid sequences, and a nucleic acid sequence that encodes a Cas protein and maintaining the cells under conditions in which the Cas protein is expressed and the Cas protein binds and modulates the one or more target nucleic acid sequences in the cell.

公开(公告)号：US10959413B2

公开(公告)日：2021-03-30

申请号：US15766551

申请日：2016-10-07

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Luhan Yang ,  Marc Guell

IPC: C12N15/63 ,  C07H21/02 ,  C07H21/04 ,  A01K67/027 ,  C12N15/113 ,  C12N15/85 ,  C12N7/00 ,  C12N9/22 ,  C12N15/11 ,  C12N15/90

Abstract: A method of modulating some or all copies of a gene in a cell is provided including introducing into a cell one or more ribonucleic acid (RNA) sequences that comprise a portion that is complementary to all or a portion of each of the one or more target nucleic acid sequences, and a nucleic acid sequence that encodes a Cas protein and maintaining the cells under conditions in which the Cas protein is expressed and the Cas protein binds and modulates the one or more target nucleic acid sequences in the cell.