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公开(公告)号:US20030194699A1
公开(公告)日:2003-10-16
申请号:US09788847
申请日:2001-02-20
申请人: Promega Corporation
发明人: Martin K. Lewis , Daniel Kephart , Richard Byron Rhodes , John William Shultz , Donna Leippe , Michelle Mandrekar , Christine Ann Andrews , James Robert Hartnett , Trent Gu , Keith V. Wood , Roy Welch
IPC分类号: C12Q001/68 , C07H021/02 , C07H021/04
CPC分类号: C12Q1/6827 , C12Q1/04 , C12Q1/66 , C12Q1/68 , C12Q1/6823 , Y10T436/24 , C12Q2565/301 , C12Q2565/1015 , C12Q2537/101 , C12Q2565/627 , C12Q2535/131 , C12Q2521/319 , C12Q2535/107
摘要: Processes are disclosed using the depolymerization of a nucleic acid hybrid to qualitatively and quantitatively analyze for the presence of predetermined nucleic acid target sequences using a multiplex assay format. Applications of those processes include the detection of single nucleotide polymorphisms, identification of single base changes, speciation, genotyping, medical marker diagnostics, and the like.
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公开(公告)号:US20010031470A1
公开(公告)日:2001-10-18
申请号:US09790417
申请日:2001-02-22
申请人: Promega Corporation
发明人: John William Shultz , Martin K. Lewis , Donna Leippe , Michelle Mandrekar , Daniel Kephart , Richard Byron Rhodes , Christine Ann Andrews , James Robert Hartnett , Trent Gu , Ryan J. Olson , Keith V. Wood , Roy Welch
IPC分类号: C12Q001/68 , C12P019/34
CPC分类号: C12Q1/6823 , C12Q1/04 , C12Q1/66 , C12Q1/68 , C12Q1/6827 , Y10T436/24 , C12Q2535/131 , C12Q2521/319 , C12Q2535/107 , C12Q2565/301 , C12Q2565/1015 , C12Q2537/101 , C12Q2565/627
摘要: Processes are disclosed using the depolymerization of a nucleic acid hybrid to qualitatively and quantitatively analyze for the presence of a predetermined nucleic acid. Applications of those processes include the detection of single nucleotide polymorphisms, identification of single base changes, speciation, determination of viral load, genotyping, medical marker diagnostics, and the like.
摘要翻译: 公开了使用核酸杂交体的解聚定性和定量分析预定核酸的存在的方法。 这些过程的应用包括单核苷酸多态性的检测,单碱基变化的鉴定,物种形成,病毒载量的测定,基因分型,医学标记诊断等。
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公开(公告)号:US20030203358A1
公开(公告)日:2003-10-30
申请号:US09780863
申请日:2001-02-09
申请人: Promega Corporation
发明人: John William Shultz , Martin K. Lewis , Donna Leippe , Michelle Mandrekar , Daniel Kephart , Richard Byron Rhodes , Christine Ann Andrews , James Robert Hartnett , Trent Gu , Ryan J. Olson , Keith V. Wood , Roy Welch
IPC分类号: C12Q001/68
CPC分类号: C12Q1/689 , C12Q1/04 , C12Q1/66 , C12Q1/6823 , C12Q1/6827 , Y10T436/24 , C12Q2565/627 , C12Q2535/131 , C12Q2521/319 , C12Q2565/301 , C12Q2565/1015 , C12Q2537/101
摘要: Processes are disclosed using the depolymerization of a nucleic acid hybrid to qualitatively and quantitatively analyze for the presence of a predetermined exogenous nucleic acid. Applications of those processes include the detection of single nucleotide polymorphisms, identification of single base changes, determination of viral load, genotyping, medical marker diagnostics, and the like.
摘要翻译: 使用核酸杂交体的解聚来定性和定量分析预定的外源核酸的存在来公开方法。 这些过程的应用包括单核苷酸多态性的检测,单碱基变化的鉴定,病毒载量的确定,基因分型,医学标记诊断等。
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公开(公告)号:US20030077621A1
公开(公告)日:2003-04-24
申请号:US10152297
申请日:2002-05-20
申请人: Promega Corporation
发明人: John William Shultz , Martin K. Lewis , Donna Leippe , Michelle Mandrekar , Daniel Kephart , Richard Byron Rhodes , Christine Ann Andrews , James Robert Hartnett , Trent Gu , Ryan J. Olson , Keith V. Wood , Roy Welch
IPC分类号: C12Q001/68 , C12P019/34
CPC分类号: C12Q1/6823 , C12Q1/04 , C12Q1/66 , C12Q1/6827 , C12Q2535/131 , C12Q2521/319 , C12Q2565/301 , C12Q2565/1015 , C12Q2537/101 , C12Q2565/627
摘要: Processes are disclosed using the depolymerization of a nucleic acid hybrid to qualitatively and quantitatively analyze for the presence of a predetermined endogenous nucleic acid. Applications of those processes include the detection of single nucleotide polymorphisms, identification of single base changes, spieciation, determination of viral load, genotyping, medical marker diagnostics, and the like.
摘要翻译: 公开了使用核酸杂交体的解聚定性和定量分析预定的内源性核酸的存在的方法。 这些过程的应用包括单核苷酸多态性的检测,单碱基变化的鉴定,间隔,病毒载量的确定,基因分型,医学标记诊断等。
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