利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

386 条记录 (耗时 0.034 秒)

    NUCLEIC ACID CLASSIFICATION
    1.
    发明申请
    NUCLEIC ACID CLASSIFICATION 审中-公开

    公开(公告)号：US20180057865A1

    公开(公告)日：2018-03-01

    申请号：US15585691

    申请日：2017-05-03

    申请人： Bio-NEMS Corporation

    发明人： David Lawrence Medin ,  Sherwood Russ Lehrman

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6827 ,  C12Q2527/101 ,  C12Q2527/107 ,  C12Q2565/607 ,  C12Q2565/627

    摘要： A method and system for classifying a target nucleic acid includes exposing, in a test system, one or more capture probes to the target nucleic acid. The one or more capture probes is attached to a surface. A first hybridization condition is established in the test system. A first degree of hybridization of the one or more capture probes with the target nucleic acid under the first hybridization condition is determined. A second hybridization condition in the test system is established. A second degree of hybridization of the one or more capture probes with the target nucleic acid under the second hybridization condition is determined and the target nucleic acid is classified by comparing the first and the second degrees of hybridization.

    Method for determining amounts of polynucleotide sequences present in cell or tissue samples
    4.
    发明授权
    Method for determining amounts of polynucleotide sequences present in cell or tissue samples 有权
    确定存在于细胞或组织样品中的多核苷酸序列的量的方法

    公开(公告)号：US09416401B2

    公开(公告)日：2016-08-16

    申请号：US10451640

    申请日：2002-01-10

    申请人： Hans Söderlund ,  Kari Kataja ,  Marja Paloheimo ,  Marja Ilmen ,  Kristiina Takkinen

    发明人： Hans Söderlund ,  Kari Kataja ,  Marja Paloheimo ,  Marja Ilmen ,  Kristiina Takkinen

    IPC分类号： C12Q1/68 ,  C12M1/34 ,  G01N33/567 ,  G01N33/00 ,  C07K1/00 ,  C07K16/00 ,  C07H21/04

    CPC分类号： C12Q1/6816 ,  C12Q2600/158 ,  C12Q2565/627 ,  C12Q2565/518 ,  C12Q2537/143

    摘要： The invention is related to a method and test kits for quantitative determination of polynucleotide amounts present in a sample. The test kit comprises organized pools with polynucleotide probes having distinct sizes and optionally provided with tracer tags or primer tags. The probes are allowed to hybridize with affinity tagged analyte polynucleotides from the sample. The result is hybrids, which can be recovered on a separation aiding tool provided with the pair of the affinity tag. After the quantitative release of the probes, the probes are either directly recorded, or if primer tagged, they are amplified and optionally provided with a tracer tag before recording. The invention provides a sensitive and quantitative determination of the amount polynucleotides present in a cell or tissue sample and allows a quantitative assessment of variations in the amounts of polynucleotides as a response to inherent changes or due to external stimuli.

    摘要翻译： 本发明涉及用于定量测定样品中存在的多核苷酸量的方法和测试试剂盒。 测试试剂盒包括具有不同大小的多核苷酸探针的有组织的池，并且任选地具有示踪剂标签或引物标签。 允许探针与来自样品的亲和标记的分析物多核苷酸杂交。 结果是混合物，其可以在具有一对亲和标签的分离辅助工具上回收。 探针的定量释放后，直接记录探针，或者如果引物标记，则它们被扩增，并且在记录之前可选地提供示踪剂标签。 本发明提供对存在于细胞或组织样品中的多核苷酸的量的敏感和定量的测定，并允许定量评估多核苷酸的量的变化，作为对固有变化或由于外部刺激的反应。

    DETECTION AND QUANTIFICATION OF BIOMOLECULES USING MASS SPECTROMETRY
    10.
    发明申请
    DETECTION AND QUANTIFICATION OF BIOMOLECULES USING MASS SPECTROMETRY 有权
    使用质谱分析的生物分子的检测和定量

    公开(公告)号：US20140235464A1

    公开(公告)日：2014-08-21

    申请号：US13766482

    申请日：2013-02-13

    申请人： SEQUENOM, INC.

    发明人： Dirk Johannes VAN DEN BOOM ,  Paul Andrew Oeth ,  Payam Mahboubi

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6823 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6872 ,  C12Q1/6883 ,  C12Q2531/113 ,  C12Q2521/319 ,  C12Q2565/627 ,  C12Q2537/143 ,  C12Q2561/101 ,  C12Q2525/161 ,  C12Q2545/114

    摘要： The present invention is directed in part to a method for detecting a target nucleic acid using detector oligonucleotides detectable by mass spectrometry. This method takes advantage of the 5′ to 3′ nuclease activity of a nucleic acid polymerase to cleave annealed oligonucleotide probes from hybridized duplexes and releases labels for detection by mass spectrometry. This process is easily incorporated into a polymerase chain reaction (PCR) amplification assay. The method also includes embodiments directed to quantitative analysis of target nucleic acids.

    摘要翻译： 本发明部分地涉及使用通过质谱法可检测的检测器寡核苷酸检测靶核酸的方法。 该方法利用核酸聚合酶的5'至3'核酸酶活性从杂交双链体切割退火寡核苷酸探针，并通过质谱法释放标记进行检测。 该方法容易地并入聚合酶链反应（PCR）扩增测定中。 该方法还包括针对靶核酸的定量分析的实施方案。