公开(公告)号：US20150126441A1

公开(公告)日：2015-05-07

申请号：US14387679

申请日：2013-03-15

申请人： Pronutria, Inc.

发明人： David Arthur Berry ,  Brett Adam Bohigian ,  Nathaniel W. Silver ,  Michael J. Hamill ,  Geoffrey von Maltzahn ,  John F. Kramarczyk ,  Rajeev Chillakuru

IPC分类号： A23J1/00 ,  A23L1/29 ,  A23L1/305 ,  C07K14/47 ,  C07K14/415

CPC分类号： A23J1/008 ,  A23L33/17 ,  A23L33/195 ,  A23L33/30 ,  A23V2002/00 ,  C07K14/415 ,  C07K14/435 ,  C07K14/47 ,  A23V2200/316 ,  A23V2250/54

摘要： Nutritive proteins comprising no phenylalanine (Phe) are provided. In some embodiments the nutritive proteins comprise at least one of a level of a) a ratio of branch chain amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of branch chain amino acid residues to total amino acid residues present in a benchmark protein; b) a ratio of leucine residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of leucine residues to total amino acid residues present in a benchmark protein; and c) a ratio of essential amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of essential amino acid residues to total amino acid residues present a benchmark protein. Also provided are nucleic acids encoding the proteins, recombinant microorganisms that make the proteins, methods of making the proteins using recombinant microorganisms, compositions that comprise the proteins, and methods of using the proteins, among other things. The compositions are useful, for example, to provide protein in the diet of subjects with a disorder characterized by accumulation of Phe in the body.

摘要翻译： 提供不含苯丙氨酸（Phe）的营养蛋白。 在一些实施方案中，营养蛋白质包含以下中的至少一种：a）支链氨基酸残基与存在于营养蛋白中的总氨基酸残基的比例等于或大于支链氨基酸残基与总氨基酸的比例 存在于基准蛋白中的酸残基; b）营养蛋白中存在的亮氨酸残基与总氨基酸残基的比例等于或大于存在于基准蛋白中的亮氨酸残基与总氨基酸残基的比例; 和c）营养蛋白中存在的必需氨基酸残基与总氨基酸残基的比例等于或大于必需氨基酸残基与总氨基酸残基的比例存在基准蛋白。 还提供编码蛋白质的核酸，制备蛋白质的重组微生物，使用重组微生物制备蛋白质的方法，包含蛋白质的组合物，以及使用蛋白质的方法等。 组合物可用于例如在具有以体内Phe聚集为特征的病症的受试者的饮食中提供蛋白质。