公开(公告)号：US09598474B2

公开(公告)日：2017-03-21

申请号：US14374127

申请日：2013-03-15

申请人： Pronutria, Inc.

发明人： David Arthur Berry ,  Brett Adam Boghigian ,  Nathaniel W. Silver ,  Geoffrey von Maltzahn ,  Rajeev Chillakuru ,  Michael Hamill ,  John F. Kramarczyk

IPC分类号： A23J3/04 ,  C07K14/435 ,  A23L1/305 ,  A61K38/17

CPC分类号： C07K14/435 ,  A23L33/17 ,  A23L33/18 ,  A23V2002/00 ,  A61K38/1709 ,  A23V2200/332 ,  A23V2250/54

摘要： Nutritive proteins are provided. In some embodiments the nutritive proteins comprise a first polypeptide sequence comprising a fragment of a naturally-occurring nutritive protein. In some embodiments the fragment comprises at least one of a) an enhanced ratio of branch chain amino acid residues to total amino acid residues present in the nutritive protein; b) an enhanced ratio of leucine residues to total amino acid residues present in the nutritive protein; and c) an enhanced ratio of essential amino acid residues to total amino acid residues present in the nutritive protein. In some embodiments, the fragment comprises at least one of a) a ratio of branch chain amino acid residues to total amino acid residues present in the nutritive protein; b) a ratio of leucine residues to total amino acid residues present in the nutritive protein; and c) a ratio of essential amino acid residues to total amino acid residues present in the nutritive protein, that is equal to or greater than the corresponding ratio present in a benchmark protein such as whey, egg or soy protein. Also provided are nucleic acids encoding the proteins, recombinant microorganisms that make the proteins, methods of making the proteins using recombinant microorganisms, compositions that comprise the proteins, and methods of using the proteins, among other things.

公开(公告)号：US20140342978A1

公开(公告)日：2014-11-20

申请号：US14337563

申请日：2014-07-22

申请人： Pronutria, Inc.

发明人： David Arthur Berry ,  Brett Adam Boghigian ,  Nathaniel W. Silver ,  Geoffrey von Maltzahn ,  Michael Hamill ,  Rajeev Chillakuru ,  John F. Kramarczyk

IPC分类号： C07K14/00 ,  A23L1/305 ,  A61K38/16

CPC分类号： C07K14/001 ,  A23J3/04 ,  A23L33/17 ,  A23L33/18 ,  A23L33/30 ,  A23V2002/00 ,  A61K31/198 ,  A61K38/00 ,  A61K38/16 ,  C07K14/415 ,  C12N15/8251 ,  Y02A50/473 ,  A23V2200/316 ,  A23V2250/55 ,  A61K2300/00

摘要： Charged nutritive proteins are provided. In some embodiments the nutritive proteins an aqueous solubility of at least 12.5 g/L at pH 7. In some embodiments the nutritive proteins an aqueous solubility of at least 50 g/L at pH 7. In some embodiments the nutritive proteins an aqueous solubility of at least 100 g/L at pH 7. In some embodiments the nutritive proteins comprise at least one of a level of a) a ratio of branch chain amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of branch chain amino acid residues to total amino acid residues present in a benchmark protein; b) a ratio of leucine residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of leucine residues to total amino acid residues present in a benchmark protein; and c) a ratio of essential amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of essential amino acid residues to total amino acid residues present in a benchmark protein. Also provided are nucleic acids encoding the proteins, recombinant microorganisms that make the proteins, methods of making the proteins using recombinant microorganisms, compositions that comprise the proteins, and methods of using the proteins, among other things.

摘要翻译： 提供带电的营养蛋白质。 在一些实施方案中，营养蛋白质在pH7下的水溶解度至少为12.5g / L。在一些实施方案中，营养蛋白质在pH7下具有至少50g / L的水溶解度。在一些实施方案中，营养蛋白质的水溶解度为 在一些实施方案中，营养蛋白质包含以下中的至少一种：a）支链氨基酸残基与存在于营养蛋白中的总氨基酸残基的比例等于或大于 支链氨基酸残基与基准蛋白中存在的总氨基酸残基的比例; b）营养蛋白中存在的亮氨酸残基与总氨基酸残基的比例等于或大于存在于基准蛋白中的亮氨酸残基与总氨基酸残基的比例; 和c）营养蛋白中存在的必需氨基酸残基与总氨基酸残基的比例等于或大于基本蛋白质中存在的必需氨基酸残基与总氨基酸残基的比例。 还提供编码蛋白质的核酸，制备蛋白质的重组微生物，使用重组微生物制备蛋白质的方法，包含蛋白质的组合物，以及使用蛋白质的方法等。

公开(公告)号：US09611298B2

公开(公告)日：2017-04-04

申请号：US14337563

申请日：2014-07-22

申请人： Pronutria, Inc.

发明人： David Arthur Berry ,  Brett Adam Boghigian ,  Nathaniel W. Silver ,  Geoffrey von Maltzahn ,  Michael Hamill ,  Rajeev Chillakuru ,  John F. Kramarczyk

IPC分类号： A23J3/04 ,  A61K38/00 ,  C07K14/00 ,  C07K14/415 ,  C12N15/82 ,  A61K38/16 ,  A61K31/198 ,  A23L33/00 ,  A23L33/17 ,  A23L33/18

CPC分类号： C07K14/001 ,  A23J3/04 ,  A23L33/17 ,  A23L33/18 ,  A23L33/30 ,  A23V2002/00 ,  A61K31/198 ,  A61K38/00 ,  A61K38/16 ,  C07K14/415 ,  C12N15/8251 ,  Y02A50/473 ,  A23V2200/316 ,  A23V2250/55 ,  A61K2300/00

摘要： Charged nutritive proteins are provided. In some embodiments the nutritive proteins an aqueous solubility of at least 12.5 g/L at pH 7. In some embodiments the nutritive proteins an aqueous solubility of at least 50 g/L at pH 7. In some embodiments the nutritive proteins an aqueous solubility of at least 100 g/L at pH 7. In some embodiments the nutritive proteins comprise at least one of a level of a) a ratio of branch chain amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of branch chain amino acid residues to total amino acid residues present in a benchmark protein; b) a ratio of leucine residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of leucine residues to total amino acid residues present in a benchmark protein; and c) a ratio of essential amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of essential amino acid residues to total amino acid residues present in a benchmark protein. Also provided are nucleic acids encoding the proteins, recombinant microorganisms that make the proteins, methods of making the proteins using recombinant microorganisms, compositions that comprise the proteins, and methods of using the proteins, among other things.

公开(公告)号：US09605040B2

公开(公告)日：2017-03-28

申请号：US14387675

申请日：2013-03-15

申请人： Pronutria, Inc.

发明人： Geoffrey von Maltzahn ,  Michael J. Hamill ,  Rajeev Chillakuru ,  John F. Kramarczyk ,  David Arthur Berry ,  Brett Adam Boghigian ,  Nathaniel W. Silver

IPC分类号： C07K14/435 ,  A61K38/00 ,  C07K14/47 ,  A23J3/04 ,  C07K14/415 ,  A23L33/175

CPC分类号： C07K14/415 ,  A23J3/04 ,  A23L33/175 ,  A23V2002/00 ,  A61K38/00 ,  C07K14/47

摘要： Nutritive proteins are provided. In some embodiments the nutritive proteins comprise at least one of a level of a) a ratio of branch chain amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of branch chain amino acid residues to total amino acid residues present in whey protein; b) a ratio of leucine residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of leucine residues to total amino acid residues present in whey protein; and c) a ratio of essential amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of essential amino acid residues to total amino acid residues present in whey protein. Also provided are nucleic acids encoding the proteins, recombinant microorganisms that make the proteins, methods of making the proteins using recombinant microorganisms, compositions that comprise the proteins, and methods of using the proteins, among other things.

公开(公告)号：US20150232520A1

公开(公告)日：2015-08-20

申请号：US14387677

申请日：2013-03-15

申请人： Pronutria, Inc.

发明人： Michael J. Hamill ,  Nathaniel W. Silver ,  Rajeev Chillakuru ,  John F. Kramarczyk ,  David Arthur Berry ,  Brett Adam Boghigian ,  Geoffrey von Maltzahn

IPC分类号： C07K14/47 ,  C07K14/415 ,  C07K14/375

CPC分类号： C07K14/47 ,  A23L33/17 ,  A23V2002/00 ,  A61K38/00 ,  C07K14/375 ,  C07K14/415 ,  C07K14/435 ,  A23V2200/332 ,  A23V2250/54

摘要： Charged nutritive proteins provided. In some embodiments the nutritive proteins an aqueous solubility of at least 12.5 g/L at pH 7. In some embodiments the nutritive proteins an aqueous solubility of at least 50 g/L at pH 7. In some embodiments the nutritive proteins an aqueous solubility of at least 100 g/L at pH 7. In some embodiments the nutritive proteins comprise at least one of a level of a) a ratio of branch chain amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of branch chain amino acid residues to total amino acid residues present in a benchmark protein; b) a ratio of leucine residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of leucine residues to total amino acid residues present in a benchmark protein; and c) a ratio of essential amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of essential amino acid residues to total amino acid residues present in a benchmark protein. Also provided are nucleic acids encoding the proteins, recombinant microorganisms that make the proteins, methods of making the proteins using recombinant microorganisms, compositions that comprise the proteins, and methods of using the proteins, among other things.

摘要翻译： 带电营养蛋白质提供。 在一些实施方案中，营养蛋白质在pH7下的水溶解度至少为12.5g / L。在一些实施方案中，营养蛋白质在pH7下具有至少50g / L的水溶解度。在一些实施方案中，营养蛋白质的水溶解度为 在一些实施方案中，营养蛋白质包含以下中的至少一种：a）支链氨基酸残基与存在于营养蛋白中的总氨基酸残基的比例等于或大于 支链氨基酸残基与基准蛋白中存在的总氨基酸残基的比例; b）营养蛋白中存在的亮氨酸残基与总氨基酸残基的比例等于或大于存在于基准蛋白中的亮氨酸残基与总氨基酸残基的比例; 和c）营养蛋白中存在的必需氨基酸残基与总氨基酸残基的比例等于或大于基本蛋白质中存在的必需氨基酸残基与总氨基酸残基的比例。 还提供编码蛋白质的核酸，制备蛋白质的重组微生物，使用重组微生物制备蛋白质的方法，包含蛋白质的组合物，以及使用蛋白质的方法等。

公开(公告)号：US20150126441A1

公开(公告)日：2015-05-07

申请号：US14387679

申请日：2013-03-15

申请人： Pronutria, Inc.

发明人： David Arthur Berry ,  Brett Adam Bohigian ,  Nathaniel W. Silver ,  Michael J. Hamill ,  Geoffrey von Maltzahn ,  John F. Kramarczyk ,  Rajeev Chillakuru

IPC分类号： A23J1/00 ,  A23L1/29 ,  A23L1/305 ,  C07K14/47 ,  C07K14/415

CPC分类号： A23J1/008 ,  A23L33/17 ,  A23L33/195 ,  A23L33/30 ,  A23V2002/00 ,  C07K14/415 ,  C07K14/435 ,  C07K14/47 ,  A23V2200/316 ,  A23V2250/54

摘要： Nutritive proteins comprising no phenylalanine (Phe) are provided. In some embodiments the nutritive proteins comprise at least one of a level of a) a ratio of branch chain amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of branch chain amino acid residues to total amino acid residues present in a benchmark protein; b) a ratio of leucine residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of leucine residues to total amino acid residues present in a benchmark protein; and c) a ratio of essential amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of essential amino acid residues to total amino acid residues present a benchmark protein. Also provided are nucleic acids encoding the proteins, recombinant microorganisms that make the proteins, methods of making the proteins using recombinant microorganisms, compositions that comprise the proteins, and methods of using the proteins, among other things. The compositions are useful, for example, to provide protein in the diet of subjects with a disorder characterized by accumulation of Phe in the body.

摘要翻译： 提供不含苯丙氨酸（Phe）的营养蛋白。 在一些实施方案中，营养蛋白质包含以下中的至少一种：a）支链氨基酸残基与存在于营养蛋白中的总氨基酸残基的比例等于或大于支链氨基酸残基与总氨基酸的比例 存在于基准蛋白中的酸残基; b）营养蛋白中存在的亮氨酸残基与总氨基酸残基的比例等于或大于存在于基准蛋白中的亮氨酸残基与总氨基酸残基的比例; 和c）营养蛋白中存在的必需氨基酸残基与总氨基酸残基的比例等于或大于必需氨基酸残基与总氨基酸残基的比例存在基准蛋白。 还提供编码蛋白质的核酸，制备蛋白质的重组微生物，使用重组微生物制备蛋白质的方法，包含蛋白质的组合物，以及使用蛋白质的方法等。 组合物可用于例如在具有以体内Phe聚集为特征的病症的受试者的饮食中提供蛋白质。

公开(公告)号：US20150087602A1

公开(公告)日：2015-03-26

申请号：US14387675

申请日：2013-03-15

申请人： Pronutria, Inc.

发明人： Geoffrey von Maltzahn ,  Michael J. Hamill ,  Rajeev Chillakuru ,  John F. Kramarczyk ,  David Arthur Berry ,  Brett Adam Boghigian ,  Nathaniel W. Silver

IPC分类号： C07K14/47 ,  C07K14/415

CPC分类号： C07K14/415 ,  A23J3/04 ,  A23L33/175 ,  A23V2002/00 ,  A61K38/00 ,  C07K14/47

摘要： Nutritive proteins are provided. In some embodiments the nutritive proteins comprise at least one of a level of a) a ratio of branch chain amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of branch chain amino acid residues to total amino acid residues present in whey protein; b) a ratio of leucine residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of leucine residues to total amino acid residues present in whey protein; and c) a ratio of essential amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of essential amino acid residues to total amino acid residues present in whey protein. Also provided are nucleic acids encoding the proteins, recombinant microorganisms that make the proteins, methods of making the proteins using recombinant microorganisms, compositions that comprise the proteins, and methods of using the proteins, among other things.

摘要翻译： 提供营养蛋白质。 在一些实施方案中，营养蛋白质包含以下中的至少一种：a）支链氨基酸残基与存在于营养蛋白中的总氨基酸残基的比例等于或大于支链氨基酸残基与总氨基酸的比例 存在于乳清蛋白中的酸残基; b）营养蛋白中存在的亮氨酸残基与总氨基酸残基的比例等于或大于乳清蛋白中存在的亮氨酸残基与总氨基酸残基的比例; 和c）营养蛋白中存在的必需氨基酸残基与总氨基酸残基的比例等于或大于乳清蛋白中存在的必需氨基酸残基与总氨基酸残基的比例。 还提供编码蛋白质的核酸，制备蛋白质的重组微生物，使用重组微生物制备蛋白质的方法，包含蛋白质的组合物，以及使用蛋白质的方法等。