公开(公告)号：US12038444B2

公开(公告)日：2024-07-16

申请号：US17828465

申请日：2022-05-31

申请人： Protein Metrics, LLC

发明人： Andrew Nichols ,  Marshall Bern ,  Yong Joo Kil ,  Eric Carlson

IPC分类号： G01N33/68 ,  H01J49/00

CPC分类号： G01N33/6848 ,  H01J49/0036 ,  H01J49/004

摘要： Methods and apparatuses for the identification and/or characterization of properties of a macromolecule based on mass spectrometry data. Specifically, described herein are methods and apparatuses for converting peptide-level data into a pseudo-intact mass spectra. Also described herein are methods and apparatuses for converting peptide-level data into a pseudo-electropherogram. The methods may be well suited for analyzing proteins and protein complexes, including estimating properties of post-translational modifications of the proteins and protein complexes. Methods may include generating a theoretical graph or spectrum based on peptide-level mass spectrometry data. In some embodiments, the theoretical graph may be a theoretical intact mass spectrum or a theoretical charge distribution spectrum.