公开(公告)号：US20220010298A1

公开(公告)日：2022-01-13

申请号：US17385460

申请日：2021-07-26

申请人： QIAGEN GmbH

发明人： Alexander Wolf ,  Sandra Hammerschmidt ,  Thorsten Voss

IPC分类号： C12N15/10 ,  C12Q1/6806

摘要： The present invention pertains to methods and kits for isolating extracellular nucleic acids from a biological sample using anion exchange particles. It was found that incorporating into the binding mixture a polyoxyalkylene fatty alcohol ether compensates performance variations that are attributable to differences in the anion exchange surface as they may occur e.g. between different lots/batches of the anion exchange particles and/or during storage of said particles. Moreover, including a polyoxyalkylene fatty alcohol ether in the binding mixture resulted in a higher purity of the obtained eluates revealing significantly less inhibition in a downstream reaction such as a PCR reaction.

公开(公告)号：US11104896B2

公开(公告)日：2021-08-31

申请号：US15577692

申请日：2016-06-10

申请人： Qiagen GmbH

发明人： Alexander Wolf ,  Sandra Hammerschmidt ,  Thorsten Voss

IPC分类号： C12N15/10 ,  C12Q1/6806

摘要： The present invention pertains to methods and kits for isolating extracellular nucleic acids from a biological sample using anion exchange particles. It was found that incorporating into the binding mixture a polyoxyalkylene fatty alcohol ether compensates performance variations that are attributable to differences in the anion exchange surface as they may occur e.g. between different lots/batches of the anion exchange particles and/or during storage of said particles. Moreover, including a polyoxyalkylene fatty alcohol ether in the binding mixture resulted in a higher purity of the obtained eluates revealing significantly less inhibition in a downstream reaction such as a PCR reaction.

公开(公告)号：US20240301396A1

公开(公告)日：2024-09-12

申请号：US18671062

申请日：2024-05-22

申请人： QIAGEN GmbH

发明人： Alexander Wolf ,  Sandra Hammerschmidt ,  Thorsten Voss

IPC分类号： C12N15/10 ,  C12Q1/6806

CPC分类号： C12N15/101 ,  C12N15/1006 ,  C12N15/1013 ,  C12Q1/6806

摘要： The present invention pertains to methods and kits for isolating extracellular nucleic acids from a biological sample using anion exchange particles. It was found that incorporating into the binding mixture a polyoxyalkylene fatty alcohol ether compensates performance variations that are attributable to differences in the anion exchange surface as they may occur e.g. between different lots/batches of the anion exchange particles and/or during storage of said particles. Moreover, including a polyoxyalkylene fatty alcohol ether in the binding mixture resulted in a higher purity of the obtained eluates revealing significantly less inhibition in a downstream reaction such as a PCR reaction.

公开(公告)号：US12031125B2

公开(公告)日：2024-07-09

申请号：US17385460

申请日：2021-07-26

申请人： QIAGEN GmbH

发明人： Alexander Wolf ,  Sandra Hammerschmidt ,  Thorsten Voss

IPC分类号： C12N15/10 ,  C12Q1/6806

CPC分类号： C12N15/101 ,  C12N15/1006 ,  C12N15/1013 ,  C12Q1/6806

摘要： The present invention pertains to methods and kits for isolating extracellular nucleic acids from a biological sample using anion exchange particles. It was found that incorporating into the binding mixture a polyoxyalkylene fatty alcohol ether compensates performance variations that are attributable to differences in the anion exchange surface as they may occur e.g. between different lots/batches of the anion exchange particles and/or during storage of said particles. Moreover, including a polyoxyalkylene fatty alcohol ether in the binding mixture resulted in a higher purity of the obtained eluates revealing significantly less inhibition in a downstream reaction such as a PCR reaction.

公开(公告)号：US20180155705A1

公开(公告)日：2018-06-07

申请号：US15577692

申请日：2016-06-10

申请人： Qiagen GmbH

发明人： Alexander Wolf ,  Sandra Hammerschmidt ,  Thorsten Voss

IPC分类号： C12N15/10 ,  C12Q1/6806

CPC分类号： C12N15/101 ,  C12N15/1006 ,  C12N15/1013 ,  C12Q1/6806

摘要： The present invention pertains to methods and kits for isolating extracellular nucleic acids from a biological sample using anion exchange particles. It was found that incorporating into the binding mixture a polyoxyalkylene fatty alcohol ether compensates performance variations that are attributable to differences in the anion exchange surface as they may occur e.g. between different lots/batches of the anion exchange particles and/or during storage of said particles. Moreover, including a polyoxyalkylene fatty alcohol ether in the binding mixture resulted in a higher purity of the obtained eluates revealing significantly less inhibition in a downstream reaction such as a PCR reaction.