公开(公告)号：US20240271105A1

公开(公告)日：2024-08-15

申请号：US18692146

申请日：2022-09-16

Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA

Inventor： Timothy D. O'Brien ,  Beth Lindborg ,  Amanda Vegoe ,  Yi Wen Chai ,  Manci Li

IPC: C12N5/071 ,  A61K35/32 ,  A61P19/02

CPC classification number: C12N5/0697 ,  A61K35/32 ,  A61P19/02 ,  C12N2500/30 ,  C12N2506/45

Abstract: A method for producing a multi-tissue organoid generally includes obtaining pluripotent stem cells (PSCs) and inducing growth of the multi-tissue organoid by culturing the harvested PSCs in suspension in a culture vessel. Another method for producing a multi-tissue organoid generally includes introducing a pluripotent stem cell into a cell culture medium comprising hyaluronic acid, transferring the pluripotent stem cell to a cell culture device that does not include a three-dimensional matrix, culturing the pluripotent stem cell in the cell culture device for at least 1 week, and producing a multi-tissue organoid comprising cartilage, bone, fibrous connective tissue, brain tissue, or epithelial tissue, or a combination thereof.