公开(公告)号：US20220304315A1

公开(公告)日：2022-09-29

申请号：US17615516

申请日：2020-05-29

Applicant: RNAISSANCE AG LLC

Inventor： John L. Killmer ,  Anil Kumar

IPC: A01N63/60 ,  C12N1/20 ,  C12N15/70 ,  C12N15/113 ,  A01P7/04 ,  A01N63/50 ,  A01N63/20

Abstract: Methods and materials for improved delivery of dsRNA are presented. In particular, methods for controlling an insect pest by stably delivering large quantities of dsRNA to the insect pest are provided. Compositions comprising a bacterium containing a large quantity of dsRNA targeting an insect gene in which the dsRNA exhibits increased stability and activity are also provided.

公开(公告)号：US11718851B2

公开(公告)日：2023-08-08

申请号：US16922948

申请日：2020-07-07

Applicant: RNAISSANCE AG LLC

Inventor： John L. Killmer ,  Patrick D. McLaughlin ,  Juan Pedro Humberto Arhancet

IPC: C12N15/113 ,  C12N15/11 ,  C07K14/005

CPC classification number: C12N15/113 ,  C07K14/005 ,  C12N15/111 ,  C12N2310/14 ,  C12N2330/50 ,  C12N2795/18122

Abstract: The invention provides methods and compositions for improved production of large quantities of unencapsidated double strand RNA (dsRNA) in vivo. The disclosed methods and compositions, comprising co-expression of genes encoding orotate phosporibosyl transferase, bacteriophage coat protein and dsRNA produce a significant improvement over current in vivo methods of producing unencapsidated dsRNA.