公开(公告)号：US20190316119A1

公开(公告)日：2019-10-17

申请号：US16400858

申请日：2019-05-01

Applicant: Raindance Technologies, Inc.

Inventor： Michael L. Samuels ,  Jeffrey Charles Olson ,  Andrew Watson ,  Keith Brown ,  Darren R. Link

IPC: C12N15/10 ,  C40B50/08 ,  C12Q1/6874 ,  G01N33/543 ,  G01N33/58

Abstract: The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.

公开(公告)号：US20160201125A1

公开(公告)日：2016-07-14

申请号：US14995744

申请日：2016-01-14

Applicant: Raindance Technologies, Inc.

Inventor： Michael L. Samuels ,  Jeffrey Charles Olson ,  Andrew Watson ,  Keith Brown ,  Darren R. Link

IPC: C12Q1/68

Abstract: The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.

Abstract translation: 本发明提供条形码文件库及其制备和使用方法，包括获得多个核酸构建体，其中每个构建体包含独特的N-聚体和功能性N-聚体，并将构建体分离成流体隔室，使得每个隔室含有一个 或更多的独特构造的副本。 本发明还提供数字PCR和数字PCR中条形码库的使用方法。

公开(公告)号：US20160289670A1

公开(公告)日：2016-10-06

申请号：US14874553

申请日：2015-10-05

Applicant: Raindance Technologies, Inc.

Inventor： Michael Samuels ,  Jeffrey Charles Olson ,  Andrew Watson ,  Keith Brown ,  Darren R. Link

IPC: C12N15/10 ,  G01N33/543

CPC classification number: C12N15/1075 ,  C12Q1/6874 ,  C40B50/08 ,  G01N33/5436 ,  G01N33/58

Abstract: The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.