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公开(公告)号：US09944977B2
公开(公告)日：2018-04-17
申请号：US14568923
申请日：2014-12-12
发明人： Darren R. Link , Michael Samuels
CPC分类号： C12Q1/6844 , C12Q1/6858 , C12Q2535/122 , C12Q2563/159 , C12Q2563/179 , C12Q2565/629
摘要： The invention generally relates to methods for distinguishing a rare genetic variation in a nucleic acid sequence.
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2.发明授权Microfluidic devices and methods of use in the formation and control of nanoreactors 有权微流体装置和用于形成和控制纳米反应器的方法公开(公告)号：US09534216B2
公开(公告)日：2017-01-03
申请号：US14248991
申请日：2014-04-09
发明人： Darren R. Link , Laurent Boitard , Jeffrey Branciforte , Yves Charles , Gilbert Feke , John Q. Lu , David Marran , Ahmadali Tabatabai , Michael Weiner , Wolfgang Hinz , Jonathan M. Rothberg
IPC分类号： G01N15/10 , C12Q1/68 , G01N21/00 , G01N21/21 , C12N15/10 , B01F13/00 , B01J19/00 , B82Y5/00 , B82Y30/00 , C07K1/04 , C40B30/04 , C40B40/04 , C40B50/08 , C40B60/08 , C40B60/12 , C40B70/00 , G01N33/536 , G01N33/68 , G01N33/543 , G01N33/50 , G01N33/00 , B01L3/00 , B01F3/08 , G01N33/542 , G01N21/64 , B01L7/00
CPC分类号： C12N15/1075 , B01F3/0807 , B01F13/0071 , B01F13/0076 , B01J19/0046 , B01J2219/00286 , B01J2219/00459 , B01J2219/00466 , B01J2219/00468 , B01J2219/00479 , B01J2219/005 , B01J2219/00576 , B01J2219/00599 , B01J2219/00702 , B01J2219/0072 , B01J2219/00743 , B01L3/5027 , B01L3/502715 , B01L3/50273 , B01L3/502746 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L3/565 , B01L7/52 , B01L2200/027 , B01L2200/0621 , B01L2200/0647 , B01L2200/0652 , B01L2200/0673 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/0896 , B01L2400/0415 , B01L2400/0424 , B01L2400/0487 , B01L2400/084 , B82Y5/00 , B82Y30/00 , C07K1/047 , C12Q1/6804 , C12Q1/6816 , C12Q1/6825 , C12Q1/6834 , C12Q1/686 , C12Q1/6869 , C12Q1/6874 , C12Q1/6876 , C12Q2525/161 , C12Q2561/119 , C12Q2563/103 , C12Q2563/107 , C12Q2563/159 , C12Q2565/537 , C12Q2565/629 , C12Q2600/16 , C40B30/04 , C40B40/04 , C40B50/08 , C40B60/08 , C40B60/12 , C40B70/00 , G01N21/6428 , G01N21/6445 , G01N21/6458 , G01N33/536 , G01N33/542 , G01N33/543 , G01N33/6845 , G01N33/6854 , G01N2500/10
摘要： The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. The invention provides for aqueous based emulsions containing uniquely labeled cells, enzymes, nucleic acids, etc., wherein the emulsions further comprise primers, labels, probes, and other reactants. An oil based carrier-fluid envelopes the emulsion library on a microfluidic device, such that a continuous channel provides for flow of the immiscible fluids, to accomplish pooling, coalescing, mixing, sorting, detection, etc., of the emulsion library.
摘要翻译： 本发明提供了可用于进行高通量筛选测定和组合化学的新型微流体装置和方法。 本发明提供了含有唯一标记的细胞，酶，核酸等的含水基乳液，其中乳液还包含引物，标记，探针和其它反应物。 油基载体流体在微流体装置上包封乳剂库，使得连续通道提供不混溶流体的流动，以实现乳液库的汇集，聚结，混合，分选，检测等。
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公开(公告)号：US20160222433A1
公开(公告)日：2016-08-04
申请号：US15019355
申请日：2016-02-09
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816 , C12Q1/6827 , C12Q1/686 , C12Q1/6883 , C12Q1/6886 , C12Q2600/172 , C12Q2563/159 , C12Q2565/629 , C12Q2537/143 , C12Q2531/113 , C12Q2535/131
摘要： The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.
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公开(公告)号：US09366632B2
公开(公告)日：2016-06-14
申请号：US13866126
申请日：2013-04-19
发明人： Darren R. Link , Qun Zhong , Andrew Watson
CPC分类号： G01N21/6486 , C12Q1/686 , C12Q2563/159 , C12Q2565/629
摘要： The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.
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5.发明申请公开(公告)号：US20150167066A1
公开(公告)日：2015-06-18
申请号：US14568923
申请日：2014-12-12
发明人： Darren R. Link , Michael Samuels
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6844 , C12Q1/6858 , C12Q2535/122 , C12Q2563/159 , C12Q2563/179 , C12Q2565/629
摘要： The invention generally relates to methods for distinguishing a rare genetic variation in a nucleic acid sequence.
摘要翻译： 本发明一般涉及区分核酸序列中罕见遗传变异的方法。
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公开(公告)号：US20150027892A1
公开(公告)日：2015-01-29
申请号：US14511892
申请日：2014-10-10
发明人： Benjamin J. Miller , John Brian Hutchison , Andrew Wilson , Jonathan William Larson , Qun Zhong , Yevgeny Yurkovetsky , Darren R. Link , Mark Weary
IPC分类号： G01N27/447
CPC分类号： G01N1/38 , B01L3/0241 , B01L3/502715 , B01L3/502784 , B01L2200/061 , B01L2200/0673 , B01L2200/143 , B01L2300/0877 , F16K99/0001 , F16K2099/0084 , F17D3/01 , G01N27/44791 , G01N2021/0353 , Y10T137/2185 , Y10T137/7761 , Y10T137/8158 , Y10T137/8593 , Y10T436/118339
摘要： The invention provides methods for assessing one or more predetermined characteristics or properties of a microfluidic droplet within a microfluidic channel, and regulating one or more fluid flow rates within that channel to selectively alter the predetermined microdroplet characteristic or property using a feedback control.
摘要翻译： 本发明提供了用于评估微流体通道内的微流体液滴的一个或多个预定特征或性质的方法，以及调节该通道内的一个或多个流体流速以使用反馈控制选择性地改变预定微滴特性或特性。
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公开(公告)号：US20180363050A1
公开(公告)日：2018-12-20
申请号：US15782001
申请日：2016-12-07
发明人： Brian Hutchison , Darren R. Link , Zuwei Ma , Qun Zhong , Aisling Steele
IPC分类号： C12Q1/6876 , C12Q1/6874
摘要： Described herein are microparticles each comprising a plurality of bound biological molecules. Further described herein is a plurality of microdroplets each comprising one or more primer vehicles. Methods of making and using these microdroplets are also reported. An exemplary microparticle is of Formula (I).
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公开(公告)号：US20150184256A1
公开(公告)日：2015-07-02
申请号：US14587365
申请日：2014-12-31
IPC分类号： C12Q1/70
CPC分类号： C12Q1/702 , C12Q1/70 , C12Q2563/159
摘要： The invention includes methods for determining the presence of a latent viral population by analyzing an RNA population from the virus with digital techniques, such as digital PCR or by sequencing cDNA produced from the RNA. The invention additional includes methods for determining the presence of latent viral populations by detecting and/or quantifying enzymes that are uniquely associated with the virus, e.g., reverse transcriptases.
摘要翻译： 本发明包括通过使用数字技术（例如数字PCR）或通过测序从RNA产生的cDNA来分析来自病毒的RNA群体来确定潜伏病毒群体的存在的方法。 本发明另外包括通过检测和/或定量与病毒唯一相关的酶（例如逆转录酶）来确定潜伏病毒种群的存在的方法。
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公开(公告)号：US20150126400A1
公开(公告)日：2015-05-07
申请号：US14596949
申请日：2015-01-14
CPC分类号： C12N15/1072 , C12Q1/6827 , C12Q1/6844 , C12Q1/6883 , C12Q1/6886 , C12Q2600/156 , C12Q2525/155 , C12Q2525/161 , C12Q2563/159 , C12Q2563/179 , C12Q2565/629 , C12Q2521/319 , C12Q2521/501 , C12Q2545/101
摘要： The invention generally relates to method for screening for a condition in a subject. In certain embodiments, methods of the invention involve obtaining a pool of nucleic acids from a sample, incubating the nucleic acids with first and second sets of binders, in which the first set binds uniquely to different regions of a target nucleic acid in the pool, the second set binds uniquely to different regions of a reference nucleic acid in the pool, and the first and second sets include different detectable labels, removing unbound binders, detecting the labels, and screening for a condition based upon results of the detecting step.
摘要翻译： 本发明一般涉及用于筛选受试者的病症的方法。 在某些实施方案中，本发明的方法包括从样品中获得核酸库，将核酸与第一组和第二组结合物孵育，其中第一组与第一组结合到泳池中的靶核酸的不同区域， 第二组与泳池中的参考核酸的不同区域唯一地结合，并且第一组和第二组包括不同的可检测标记，去除未结合的结合物，检测标记，以及基于检测步骤的结果筛选病症。
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公开(公告)号：US20140329239A1
公开(公告)日：2014-11-06
申请号：US14264686
申请日：2014-04-29
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816 , C12Q1/6827 , C12Q1/686 , C12Q1/6883 , C12Q1/6886 , C12Q2600/172 , C12Q2563/159 , C12Q2565/629 , C12Q2537/143 , C12Q2531/113 , C12Q2535/131
摘要： The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.
摘要翻译： 本发明一般涉及基于液滴的数字PCR和使用其进行目标核酸分析的方法。 在某些实施方案中，本发明的方法包括形成含有平均单个靶核酸的样品液滴，扩增液滴中的靶，不包含来自靶标的扩增子的液滴和来自靶标变体的扩增子，并分析靶扩增子 。
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