公开(公告)号：US06088097A

公开(公告)日：2000-07-11

申请号：US231902

申请日：1999-01-14

Applicant: Rainer Uhl

Inventor： Rainer Uhl

IPC: G02B21/00 ,  G01J21/64

CPC classification number: G02B21/0068 ,  G02B21/0036 ,  G02B21/0072 ,  G02B21/0076 ,  G02B21/0084

Abstract: A point-scanning luminescent microscope, especially for studying biological objects, has at least one collimated light source for producing an excitation light beam, an optical arrangement which focuses the light of the excitation light source on an object to be studied, at least one detector arrangement for acquiring light emitted by the object, an optical arrangement which collects the light emitted by the object and supplies it to the detector arrangement, and a scanner arrangement which causes relative movement between the scanning light beam and the object in at least two directions. The scanner arrangement has piezoactuators for achieving scanning movements between the scanning light beam and object. The detector arrangement can have a surface sensor which forms a confocal diaphragm. The light source can be designed to deliver rectangular pulses. When the microscope is designed for twin-photon fluorescent microscopy, an objective lens is provided for illuminating the object and can be is used, at the same time, for collecting some of the photons emitted by the object, and a second detector is provided behind a condenser lens of the objective lens.

Abstract translation: 特别是用于研究生物体的点扫描发光显微镜具有至少一个用于产生激发光束的准直光源，将激发光源的光聚焦在待研究物体上的光学装置，至少一个检测器 用于获取由物体发射的光的装置，收集由物体发射的光并将其提供给检测器装置的光学装置;以及扫描器装置，其在至少两个方向上引起扫描光束和物体之间的相对移动。 扫描器装置具有压电致动器，用于实现扫描光束和物体之间的扫描运动。 检测器装置可以具有形成共焦隔膜的表面传感器。 光源可以设计成传递矩形脉冲。 当显微镜设计用于双光子荧光显微镜时，提供用于照射物体的物镜，并且可以同时用于收集由物体发射的一些光子，并且在后面提供第二检测器 物镜的聚光透镜。

公开(公告)号：US07706043B2

公开(公告)日：2010-04-27

申请号：US12506431

申请日：2009-07-21

Applicant: Rainer Uhl ,  Christian Seebacher ,  Rainer Daum

Inventor： Rainer Uhl ,  Christian Seebacher ,  Rainer Daum

IPC: G02B21/00 ,  G02B21/06

CPC classification number: G02B21/0032 ,  G02B21/0044

Abstract: A device for confocal observation of a specimen, having a mask, which is located in the illumination beam path and the image beam path and is rotatable around a central axis, the mask being provided with openings for generating an illumination pattern moving on the specimen, an arrangement of a plurality of focusing microoptics which is adjusted to the geometric arrangement of the openings of the mask and to the rotation of the mask in order to concentrate the illumination light by each of the microoptics into a respective one of the openings of the mask, and a beam splitter for separating light from the specimen from illumination light, wherein the beam splitter is arranged in the beam path between the mask and the arrangement of the microoptics, and wherein an optical arrangement is provided in the beam path between the mask and the arrangement of the microoptics.

Abstract translation: 一种用于共焦观察试样的装置，具有掩模，该掩模位于照明光束路径和图像光束路径中并且可围绕中心轴线旋转，所述掩模设置有用于产生在样本上移动的照明图案的开口， 多个聚焦微光学的布置，其被调整到掩模的开口的几何布置和掩模的旋转，以便通过每个微光学将照明光集中到掩模的相应的一个开口中 以及分束器，用于将来自所述样本的光与照明光分离，其中所述分束器布置在所述掩模和所述微光学装置之间的光束路径中，并且其中光学布置设置在所述掩模和 微光学的安排。

公开(公告)号：US20070008516A1

公开(公告)日：2007-01-11

申请号：US11521001

申请日：2006-09-14

Applicant: Rainer Uhl

Inventor： Rainer Uhl

IPC: G01J1/00

CPC classification number: G02B6/4206 ,  G02B6/0006 ,  G02B6/29362 ,  G02B6/4214 ,  G02B6/4298 ,  G02B21/125 ,  G02B27/0905 ,  G02B27/145

Abstract: A microscope having a device for merging different light beams, guiding them to a main beam outlet, and scrambling them in a way that a homogeneous intensity distribution is achieved there. When critical illumination is employed this carries over to a homogeneous illumination of the object plane of the microscope within the boundaries of a field of view determined by the boundaries of the main beam outlet. The device comprises a plurality of optical elements stacked in a row. The optical elements have light guiding properties and plane mating surfaces in a connection area between two adjacent optical elements, wherein the mating surfaces are inclined and serve as beam splitting surfaces.

Abstract translation: 具有用于合并不同光束的装置的显微镜，将它们引导到主光束出口，并以其中实现均匀的强度分布的方式进行加扰。 当采用临界照明时，在由主光束出口的边界确定的视场的边界内，将显微镜的物平面照射到均匀照明。 该装置包括一排堆叠的多个光学元件。 光学元件在两个相邻光学元件之间的连接区域中具有导光性能和平面配合表面，其中配合表面倾斜并用作分束表面。

公开(公告)号：US06633381B2

公开(公告)日：2003-10-14

申请号：US09829065

申请日：2001-04-10

Applicant: Rainer Uhl

Inventor： Rainer Uhl

IPC: G01J330

CPC classification number: G02B21/0076

Abstract: A fluorescence-measuring device for scanning a specimen (36, 226) with a selection element (26, 206). Excitation light is coupled to an excitation beam path and guided to the specimen surface. Fluorescent light, which is emitted by the specimen surface, crosses the excitation beam path in a reverse direction and at the same time is decoupled from the excitation beam path. In the beam path, there is a first dispersive element (30, 210) so that the fluorescent light emitted by the specimen surface can strike the selection element spectrally split. The selection element includes a first area (48, 240) and a second area (26, 242) allowing selection between the excitation and fluorescent light. According to a first aspect, a spatial extension of a transmission area (26) is adjustable in order to adjust the transmitted wavelength range of the emission light. According to a second aspect, the selection element (206) makes it possible to operate with a coherent excitation having several laser lines. A two-dimensional confocal measurement arrangement can be accomplished and the emission light separated from the excitation light can be detected with spectral resolution.

Abstract translation: 用于用选择元素（ 26,206 ）扫描样本的荧光测量装置（ 36,226 ）。 激发光耦合到激发光束路径并被引导到样品表面。 由样品表面发射的荧光灯在相反方向上穿过激发光束路径，同时与激发光束路径解耦。 在光束路径中，存在第一色散元件（ 30,210 ），使得由样品表面发射的荧光可以使光谱分裂的选择元件发生。 选择元素包括第一区域（ 48,240 ）和第二区域（ 26,242 ）， 激发和荧光灯。 根据第一方面，传输区域（ 26 ）的空间扩展是可调节的，以便调整发射光的透射波长范围。 根据第二方面，选择元件（ 206 ）使得可以用具有几条激光线的相干激励来操作。 可以实现二维共焦测量装置，并且可以用光谱分辨率检测与激发光分离的发射光。

公开(公告)号：US07936502B2

公开(公告)日：2011-05-03

申请号：US11844446

申请日：2007-08-24

Applicant: Rainer Uhl

Inventor： Rainer Uhl

IPC: G02B21/06

CPC classification number: G02B21/24

Abstract: A microscope device comprising a microscope objective and a plate-like body limited by a flat top face and a flat lower face essentially parallel thereto, with the microscope objective being connected to the plate-like body, and with a portion of a beam path of the microscope device extending above and/or below the plate-like body, wherein another portion of the beam path of the microscope device extends, essentially parallel to the top face and the lower face, within a recess within the plate-like body.

Abstract translation: 一种显微镜装置，包括显微镜物镜和由平坦顶面限定的板状物体和与其基本平行的平坦下表面，显微镜物镜连接到板状物体，并且一部分光束路径 所述显微镜装置在所述板状体的上方和/或下方延伸，其中所述显微镜装置的所述光束路径的另一部分在所述板状体内的凹部内延伸，基本上平行于所述顶面和所述下表面。

公开(公告)号：US07573635B2

公开(公告)日：2009-08-11

申请号：US11558122

申请日：2006-11-09

Applicant: Rainer Uhl

Inventor： Rainer Uhl

IPC: G02B21/06 ,  G02B21/00

CPC classification number: G02B21/0048 ,  G02B21/0076 ,  G02B21/06 ,  G02B21/18

Abstract: There is provided a method for a microscope device for optionally examining a sample by at least a first light beam bundle from a first direction or a second light beam bundle from a second direction different to the first direction, having a microscope objective and a beam deflection element, wherein the beam deflection element is operable by a drive in order to optionally couple the first light beam bundle or the second light beam bundle into the microscope objective, and wherein the beam deflection element is rotatable by the drive in order to optionally change at least an exit direction of the first light beam bundle from the beam deflection element if the first light beam bundle is coupled into the microscope objective.

Abstract translation: 提供了一种显微镜装置的方法，用于通过来自第一方向的至少第一光束或来自与第一方向不同的第二方向的第二光束束可选地检查样品，具有显微镜物镜和光束偏转 元件，其中所述光束偏转元件可由驱动器操作，以便可选地将所述第一光束束或所述第二光束束耦合到所述显微镜物镜中，并且其中所述光束偏转元件可由所述驱动器旋转，以便可选地在 如果第一光束束耦合到显微镜物镜中，则来自光束偏转元件的至少一个第一光束束的出射方向。

公开(公告)号：US07423806B2

公开(公告)日：2008-09-09

申请号：US10547379

申请日：2004-02-19

Applicant: Rainer Uhl ,  Andreas Kantwerg

Inventor： Rainer Uhl ,  Andreas Kantwerg

IPC: G02B21/00

CPC classification number: G02B21/361 ,  G02B7/006 ,  G02B21/025 ,  G02B21/241 ,  G02B21/248

Abstract: A microscope system having an objective lens (10) defining a central optical axis (14) of the microscope system; an optical beam hub unit (18, 20) having a center coinciding with the central optical axis, a plurality of optical beam ports (24, 26, 30, 32; 214) arranged radially around the central optical axis, a beam multiplexer system (22, 28, 222) arranged in the center of the hub unit, and a device for rotating the beam multiplexer system around the central optical axis for alternatively selecting at least one of the beam ports. The beam ports include at least two elements selected any one of input ports, output ports and dual input/output ports.

Abstract translation: 具有限定所述显微镜系统的中心光轴（14）的物镜（10）的显微镜系统; 具有与中心光轴重合的中心的光束集线器单元（18,20），围绕中心光轴布置的多个光束端口（24,26,30,32; 214），光束多路复用器系统 22,28,222），以及用于围绕所述中心光轴旋转所述光束多路复用器系统以便交替地选择所述光束端口中的至少一个的装置。 波束端口包括至少两个选择输入端口，输出端口和双输入/输出端口中的任何一个的元件。

公开(公告)号：US07327514B2

公开(公告)日：2008-02-05

申请号：US10547370

申请日：2004-03-01

Applicant: Rainer Uhl ,  Hartmann Harz

Inventor： Rainer Uhl ,  Hartmann Harz

IPC: G02B21/32

CPC classification number: G02B21/361 ,  G02B7/006 ,  G02B21/025 ,  G02B21/241 ,  G02B21/248

Abstract: A microscope system having a plurality of exchangeable objective lenses (10); an objective lens changeover element (18) for supporting each of the objective lenses which is operable for placing a selected one of said objective lenses into an optical axis (22) of the microscope system, wherein each objective lens is supported by the changeover element in such a manner that each objective lens is moveable coaxially with respect to the optical axis and relative to the changeover element; and an actuator element (20, 40) for moving the selected one of the objective lenses coaxially relative to the optical axis and relative to the changeover element for focusing the selected one of the objective lenses relative to a specimen (12).

Abstract translation: 一种具有多个可更换物镜（10）的显微系统; 用于支撑每个物镜的物镜切换元件（18），所述物镜可操作用于将所选择的所述物镜放置在所述显微镜系统的光轴（22）中，其中每个物镜由所述转换元件支撑 这样一种方式使得每个物镜相对于光轴和相对于切换元件同轴地可移动; 以及用于相对于所述光轴相对于所述转换元件相对于所述转换元件同轴地移动所选择的一个所述物镜的致动器元件（20,40），用于相对于所述样本（12）聚焦所选择的一个所述物镜。

公开(公告)号：US07196787B2

公开(公告)日：2007-03-27

申请号：US10790684

申请日：2004-03-03

Applicant: Rainer Uhl ,  Michael Hartl

Inventor： Rainer Uhl ,  Michael Hartl

IPC: G02B21/00 ,  G01J3/30

CPC classification number: G01N21/6458 ,  G01N21/552 ,  G01N21/648 ,  G02B21/00 ,  G02B21/16

Abstract: An apparatus for total internal reflection microscopy of a sample, comprising a microscope objective lens; an excitation beam path for passing light through the objective lens to said sample; and a coupling element arranged in a back focal plane of the objective lens or in a plane which is conjugate to said back focal plane; said coupling element comprising a first area for relaying light to the objective lens for total internal reflection illumination of said sample and a second area; wherein said second area is capable of separating light emitted by said sample and passing through said excitation beam path in reverse direction from said excitation beam path; wherein said second area is spatially separate from said first area and does not overlap with said first area; and wherein a distance between said optical axis of the objective lens and that boundary of said first area which is nearer to said optical axis of the objective lens is selected such that the light beams passing from said first area into the objective lens are imaged by the objective lens at angles onto said sample for which total reflection of these light beams occurs.

Abstract translation: 一种用于样品的全内反射显微镜的装置，包括显微镜物镜; 激发光束路径，用于将光通过物镜传送到所述样品; 以及耦合元件，其布置在所述物镜的后焦平面中或者在与所述后焦平面共轭的平面中; 所述耦合元件包括用于将光中继到物镜的第一区域，用于所述样品的全内反射照明和第二区域; 其中所述第二区域能够分离由所述样品发射的光并且从所述激发光束路径反向穿过所述激发光束路径; 其中所述第二区域在空间上与所述第一区域分离并且不与所述第一区域重叠; 并且其中选择所述物镜的所述光轴与所述第一区域的接近所述物镜的所述光轴的所述边界之间的距离，使得从所述第一区域通过所述物镜的光束被所述物镜 在所述样品上的角度的物镜，其中出现这些光束的全反射。

公开(公告)号：US09759901B2

公开(公告)日：2017-09-12

申请号：US14008473

申请日：2012-04-16

Applicant: Rainer Uhl ,  Rainer Daum ,  Xaver Voegele

Inventor： Rainer Uhl ,  Rainer Daum ,  Xaver Voegele

IPC: G02B21/16 ,  G01N21/64 ,  G02B21/36 ,  G02B21/18

CPC classification number: G02B21/16 ,  G01N21/64 ,  G01N21/6458 ,  G02B21/18 ,  G02B21/361

Abstract: The invention relates to a microscope arrangement provided with: a microscope (10) having at least two optical outputs (12, 14) for outputting a fluorescence signal and a switching arrangement (16) for switching the output of the fluorescence signal between the optical outputs; a beam splitter arrangement (18); optical elements (28, 30) for generating a separate partial beam path (24, 26) associated with each output in such a way that the respective fluorescence signal of each of the outputs is superimposed at the beam splitter arrangement after passing through the respective partial beam path; and also at least two optical detectors (20, 22), wherein for each of the partial beam paths, one of the detectors is located behind the beam splitter, seen from the microscope, in reflection and another of the detectors is located behind the beam splitter arrangement, seen from the microscope, in transmission.