公开(公告)号：US20110191904A1

公开(公告)日：2011-08-04

申请号：US12972408

申请日：2010-12-17

Applicant: Rajesh Kumar ,  Henry T. Nguyen

Inventor： Rajesh Kumar ,  Henry T. Nguyen

IPC: C12N15/82 ,  A01H5/00

CPC classification number: C12N15/8247

Abstract: Cytochrome b5 (Cb5) is a haem-binding protein located in the endoplasmic reticulum (ER) and the outer mitochondrial membranes of higher eukaryotes. In higher plants, animals, and fungi, the ER resident Cb5 has been shown to play a role in desaturation of acyl CoA fatty acids. Higher plants Cb5 isoforms from plants such as soybean or Arabidopsis are capable of modulating omega-3 desaturation. Co-expression of certain Cb5 isoforms with FAD3 in a host plant results in increased production of seed oil content as well as altered ratio between different fatty acids. It is also disclosed here that overexpression of Yarrowia ACL enzymes in the plastids of a host plant helps boost the synthesis of acetyl CoA, which in turn, may lead to increased synthesis of fatty acids and enhanced oil accumulation in the seeds.

Abstract translation: 细胞色素b5（Cb5）是位于内质网（ER）中的血液结合蛋白和高等真核生物的外线粒体膜。 在高等植物，动物和真菌中，已经显示ER居民Cb5在酰基辅酶A脂肪酸的去饱和中起作用。 来自植物如大豆或拟南芥的高等植物Cb5异构体能够调节ω-3去饱和。 某些Cb5异构体与宿主植物中的FAD3的共表达导致种子油含量的产量增加以及不同脂肪酸之间的比例改变。 这里还公开了耶氏酵母属ACL酶在宿主植物的质体中的过表达有助于促进乙酰辅酶A的合成，这反过来可能导致脂肪酸的合成增加并增强种子中的油积聚。

公开(公告)号：US08653328B2

公开(公告)日：2014-02-18

申请号：US12972408

申请日：2010-12-17

Applicant: Rajesh Kumar ,  Henry T. Nguyen

Inventor： Rajesh Kumar ,  Henry T. Nguyen

IPC: C12N15/87 ,  C12N15/82 ,  C12N15/29 ,  A01H5/00

CPC classification number: C12N15/8247

Abstract: Cytochrome b5 (Cb5) is a haem-binding protein located in the endoplasmic reticulum (ER) and the outer mitochondrial membranes of higher eukaryotes. In higher plants, animals, and fungi, the ER resident Cb5 has been shown to play a role in desaturation of acyl CoA fatty acids. Higher plants Cb5 isoforms from plants such as soybean or Arabidopsis are capable of modulating omega-3 desaturation. Co-expression of certain Cb5 isoforms with FAD3 in a host plant results in increased production of seed oil content as well as altered ratio between different fatty acids. It is also disclosed here that overexpression of Yarrowia ACL enzymes in the plastids of a host plant helps boost the synthesis of acetyl CoA, which in turn, may lead to increased synthesis of fatty acids and enhanced oil accumulation in the seeds.

Abstract translation: 细胞色素b5（Cb5）是位于内质网（ER）和高等真核生物的外线粒体膜的血液结合蛋白。 在高等植物，动物和真菌中，已经显示ER居民Cb5在酰基辅酶A脂肪酸的去饱和中起作用。 来自植物如大豆或拟南芥的高等植物Cb5异构体能够调节ω-3去饱和。 某些Cb5异构体与宿主植物中的FAD3的共表达导致种子油含量的产量增加以及不同脂肪酸之间的比例改变。 这里还公开了耶氏酵母属ACL酶在宿主植物的质体中的过表达有助于促进乙酰辅酶A的合成，这反过来可能导致脂肪酸的合成增加和种子中油的积聚增加。

公开(公告)号：US20110119792A1

公开(公告)日：2011-05-19

申请号：US12966981

申请日：2010-12-13

Applicant: Henry T. Nguyen ,  Babu Valliyodan

Inventor： Henry T. Nguyen ,  Babu Valliyodan

IPC: C12N15/82 ,  A01H5/00

CPC classification number: C12N15/8273 ,  C07K14/415 ,  C12N15/8201 ,  C12N15/8227 ,  C12N15/8246 ,  C12N15/8247 ,  C12N15/8267

Abstract: Microarrays are employed to analyze soybean transcriptome under water stress conditions in different regions of the root at vegetative stage. Drought responsive genes and transcription factors are identified which may be used for enhancing drought tolerance in soybean or other plants through genetic/metabolic engineering. This disclosure pertains to nucleic acid molecules isolated from soybean and maize that encode polypeptides that may be important for drought tolerance. The disclosure also relates to methods of using these genes from soybean in transgenic plants to confer the desired agronomic traits, and to use such genes or regulatory elements thereof to assist germplasm enhancement by molecular breeding or to identify other factors or chemicals that may enhance a plant's capability to grow under drought conditions.

Abstract translation: 微阵列用于在营养阶段的根部的不同区域的水胁迫条件下分析大豆转录组。 鉴定出干旱响应基因和转录因子，其可以通过遗传/代谢工程用于增强大豆或其他植物的耐旱性。 本公开涉及从大豆和玉米分离的编码可能对耐旱性重要的多肽的核酸分子。 本公开还涉及在转基因植物中使用来自大豆的这些基因用于赋予所需农学性状的方法，并且使用这些基因或其调控元件来帮助通过分子育种增强种质，或鉴定其它因素或化学物质，其可以增强植物的 在干旱条件下生长的能力。

公开(公告)号：US20150059022A1

公开(公告)日：2015-02-26

申请号：US14528229

申请日：2014-10-30

Applicant: Henry T. Nguyen ,  Babu Valliyodan

Inventor： Henry T. Nguyen ,  Babu Valliyodan

IPC: C12N15/82

CPC classification number: C12N15/8273 ,  C07K14/415 ,  C12N15/8201 ,  C12N15/8227 ,  C12N15/8246 ,  C12N15/8247 ,  C12N15/8267

Abstract: Microarrays are employed to analyze soybean transcriptome under water stress conditions in different regions of the root at vegetative stage. Drought responsive genes and transcription factors are identified which may be used for enhancing drought tolerance in soybean or other plants through genetic/metabolic engineering. This disclosure pertains to nucleic acid molecules isolated from soybean and maize that encode polypeptides that may be important for drought tolerance. The disclosure also relates to methods of using these genes from soybean in transgenic plants to confer the desired agronomic traits, and to use such genes or regulatory elements thereof to assist germplasm enhancement by molecular breeding or to identify other factors or chemicals that may enhance a plant's capability to grow under drought conditions.

Abstract translation: 微阵列用于在营养阶段的根部的不同区域的水胁迫条件下分析大豆转录组。 鉴定出干旱响应基因和转录因子，其可以通过遗传/代谢工程用于增强大豆或其他植物的耐旱性。 本公开涉及从大豆和玉米分离的编码可能对耐旱性重要的多肽的核酸分子。 本公开还涉及在转基因植物中使用来自大豆的这些基因用于赋予所需农学性状的方法，并且使用这些基因或其调控元件来帮助通过分子育种增强种质，或鉴定其它因素或化学物质，其可以增强植物的 在干旱条件下生长的能力。

公开(公告)号：US07732667B2

公开(公告)日：2010-06-08

申请号：US10928992

申请日：2004-08-27

Applicant: Henry T. Nguyen ,  Joel A. Kreps

Inventor： Henry T. Nguyen ,  Joel A. Kreps

IPC: C12N15/82 ,  C12N15/29 ,  A01H5/00

CPC classification number: C07K14/415 ,  C12N15/8271 ,  C12N15/8273

Abstract: The present subject matter provides a method for enhancing the abiotic stress tolerance of a plant. Polynucleotides isolated from rice (Oryza sativa) and encoding polypeptides for abiotic stress tolerance are also described.

Abstract translation: 本发明提供了一种用于增强植物的非生物胁迫耐受性的方法。 还描述了从水稻（Oryza sativa）分离并编码多肽用于非生物胁迫耐受性的多核苷酸。

公开(公告)号：US08692064B2

公开(公告)日：2014-04-08

申请号：US12896864

申请日：2010-10-02

Applicant: Henry T. Nguyen ,  David A. Sleper ,  James G. Shannon ,  Tri D. Vuong ,  Xiaolei Wu

Inventor： Henry T. Nguyen ,  David A. Sleper ,  James G. Shannon ,  Tri D. Vuong ,  Xiaolei Wu

IPC: A01H1/00 ,  A01H1/04

CPC classification number: C12N15/8285 ,  A01H1/00 ,  A01H5/10 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156

Abstract: Several QTLs that are genetically linked to resistance to soybean cyst nematode (SCN) are disclosed. These QTLs have been mapped to genomic regions on Chrs. 4, 8, 10, 11, 18, and 20 of soybean, G. max. Candidate genes underlying these QTLs as defined by the flanking markers, as well as genetic markers associated with these QTLs are also disclosed. These markers can be utilized for introgressing SCN resistance into non-resistant soybean germplasm. The unique resistance genes can be introduced into a non-resistant plant by marker-assisted selection (MAS) or by transgenic methods.

Abstract translation: 披露了与大豆胞囊线虫（SCN）抗性基因相关的几个QTL。 这些QTL已被映射到Chrs的基因组区域。 4，8，10，11，18和20，大豆G. 还公开了由侧翼标记定义的这些QTL的候选基因以及与这些QTL相关的遗传标记。 这些标记物可以用于将抗性大肠杆菌SCN耐药性侵入非抗性大豆种质。 可以通过标记辅助选择（MAS）或转基因方法将独特的抗性基因引入非抗性植物。

公开(公告)号：US20120198587A1

公开(公告)日：2012-08-02

申请号：US13381448

申请日：2010-06-30

Applicant: Henry T. Nguyen ,  Gary Stacey ,  Dong Xu ,  Jianlin Cheng ,  Trupti Joshi ,  Marc Libault ,  Babu Valliyodan

Inventor： Henry T. Nguyen ,  Gary Stacey ,  Dong Xu ,  Jianlin Cheng ,  Trupti Joshi ,  Marc Libault ,  Babu Valliyodan

IPC: C12N15/82 ,  A01H5/00

CPC classification number: C07K14/415

Abstract: Gene expression is controlled at the transcriptional level by very diverse group of proteins called transcription factors (TFs). 5671 soybean (Glycine max) genes have been identified and disclosed as putative transcription factors through mining of soybean genome sequences. Distinct classes of the TFs are also disclosed which may be expressed and or function in a manner that is tissue specific, developmental stage specific, biotic and/or abiotic stress specific. Manipulation and/or genetic engineering of specific transcription factors may improve the agronomic performance or nutritional quality of plants. Transgenic plants expressing a select number of these TFs are disclosed. These transgenic plants show some promising traits, such as improving the capability of the plant to grow and reproduce under drought conditions.

Abstract translation: 基因表达通过称为转录因子（TF）的非常多样化的蛋白质组在转录水平下控制。 已经通过大豆基因组序列的开采，鉴定了5671个大豆（Glycine max）基因并将其公开为推定的转录因子。 还公开了TF的不同类别，其可以以组织特异性，发育阶段特异性，生物和/或非生物胁迫特异性的方式表达和/或起作用。 特定转录因子的操作和/或遗传工程可以改善植物的农艺性能或营养品质。 公开了表达选择数目的这些TF的转基因植物。 这些转基因植物显示出一些有希望的性状，例如提高植物在干旱条件下生长和繁殖的能力。

公开(公告)号：US20090210968A1

公开(公告)日：2009-08-20

申请号：US12138392

申请日：2008-06-12

Applicant: Henry T. Nguyen ,  Babu Valliyodan

Inventor： Henry T. Nguyen ,  Babu Valliyodan

IPC: C12N15/29 ,  A01H5/00

CPC classification number: C12N15/8273 ,  C07K14/415

Abstract: Microarrays are employed to analyze soybean transcriptions under water stress conditions in both root and leaf tissues at vegetative stage. Novel drought responsive genes may be thus identified and may be used for enhancing drought tolerance in soybean or other plants through genetic/metabolic engineering. This disclosure pertains to nucleic acid molecules isolated from Soybean that encode polypeptides that may be important for drought tolerance. The disclosure also relates to methods of using these genes from soybean in transgenic plants to confer the desired agronomic traits, and to use such genes or regulatory elements thereof to assist germplasm enhancement by molecular breeding or to identify other factors or chemicals that may enhance a plant's capability to grow under drought conditions.

Abstract translation: 微阵列用于在营养阶段的根和叶组织的水胁迫条件下分析大豆转录。 因此，可以鉴定出新的干旱反应性基因，并且可以通过遗传/代谢工程用于增强大豆或其他植物的耐旱性。 本公开涉及从大豆分离的编码可能对耐旱性重要的多肽的核酸分子。 本公开还涉及在转基因植物中使用来自大豆的这些基因用于赋予所需农学性状的方法，并且使用这些基因或其调控元件来帮助通过分子育种增强种质，或鉴定其它因素或化学物质，其可以增强植物的 在干旱条件下生长的能力。

公开(公告)号：US20080184393A1

公开(公告)日：2008-07-31

申请号：US12023237

申请日：2008-01-31

Applicant: Zhanyuan J. Zhang ,  Xinlu Chen ,  Henry T. Nguyen

Inventor： Zhanyuan J. Zhang ,  Xinlu Chen ,  Henry T. Nguyen

IPC: C12N15/82 ,  C12N15/11 ,  A01H5/00

CPC classification number: C12N15/8205 ,  C12N9/1085 ,  C12N15/8216

Abstract: Agrobacterium-mediated plant transformation with a transgene of interest is accomplished with high frequency of transformation by using a specially constructed vector. The vector combines the transgene of interest with an autoregulating promoter, such as the PSAG12 promoter, that controls expression of an IPT coding region. The IPT coding region may be replaced by another ORF coding for the expression of a polypeptide affecting at least one plant cell cycle pathway selected from the group consisting of cytokinin, auxin, and sugar pathways.

Abstract translation: 用感兴趣的转基因进行农杆菌介导的植物转化通过使用特异构建的载体以高频率的转化来实现。 该载体将感兴趣的转基因与控制IPT编码区表达的自动调节启动子（如PNASAG12启动子）相结合。 IPT编码区可以被编码影响至少一种选自细胞分裂素，生长素和糖途径的植物细胞周期途径的多肽的表达的另一个ORF替代。