公开(公告)号：US20060011858A1

公开(公告)日：2006-01-19

申请号：US10967328

申请日：2004-10-19

申请人： Ralf Engelmann ,  Joerg-Michael Funk ,  Joerg Steinert ,  Bernhard Zimmermann ,  Stefan Wilhelm ,  Joerg Engel ,  Ulrich Meisel

发明人： Ralf Engelmann ,  Joerg-Michael Funk ,  Joerg Steinert ,  Bernhard Zimmermann ,  Stefan Wilhelm ,  Joerg Engel ,  Ulrich Meisel

IPC分类号： G01N21/64

CPC分类号： G02B21/0076 ,  G02B21/0048

摘要： Process for the observation of at least one sample region with a light raster microscope by a relative movement between the illumination light and sample via first scanning means along at least one scanning axis essentially perpendicular to the illumination axis wherein at an angle to the plane of the relative movement, preferably perpendicular thereto, second scanning means are moved and an image acquisition takes place by the movement of the first and second scanning means being coupled and a three-dimensional sampling movement being done by the illumination of the sample wherein the second scanning means are coupled to the movement of the first scanning means in such a manner that straight and/or curved lines and/or plane and/or curved surfaces are scanned which are extended along at least one scanning direction of the first scanning means as well as along the scanning direction of the second scanning means.

摘要翻译： 用于通过第一扫描装置沿着基本上垂直于照明轴线的至少一个扫描轴的照明光和样品之间的相对运动用光栅显微镜观察至少一个样品区域的方法，其中与至少一个样品区域的平面成角度 相对运动，优选地与其垂直，第二扫描装置被移动，并且通过被耦合的第一和第二扫描装置的运动而进行图像采集，并且三维采样运动是通过样品的照射完成的，其中第二扫描装置 耦合到第一扫描装置的运动，使得沿着第一扫描装置的至少一个扫描方向延伸的直线和/或曲线和/或平面和/或曲面被扫描，以及沿着 第二扫描装置的扫描方向。

公开(公告)号：US20070131875A1

公开(公告)日：2007-06-14

申请号：US11590851

申请日：2006-11-01

申请人： Ralf Engelmann ,  Joerg-Michael Funk ,  Joerg Steinert ,  Bernhard Zimmermann ,  Stefan Wilhelm ,  Joerg Engel ,  Ulrich Meisel

发明人： Ralf Engelmann ,  Joerg-Michael Funk ,  Joerg Steinert ,  Bernhard Zimmermann ,  Stefan Wilhelm ,  Joerg Engel ,  Ulrich Meisel

IPC分类号： G01N21/64

CPC分类号： G02B21/0076 ,  G02B21/0048

摘要： Process for the observation of at least one sample region with a light raster microscope by a relative movement between the illumination light and sample via first scanning means along at least one scanning axis essentially perpendicular to the illumination axis wherein at an angle to the plane of the relative movement, preferably perpendicular thereto, second scanning means are moved and an image acquisition takes place by the movement of the first and second scanning means being coupled and a three-dimensional sampling movement being done by the illumination of the sample wherein the second scamming means are coupled to the movement of the first scanning means in such a manner that straight and/or curved lines and/or plane and/or curved surfaces are scanned which are extended along at least one scanning direction of the first scanning means as well as along the scanning direction of the second scanning means.

摘要翻译： 用于通过第一扫描装置沿着基本上垂直于照明轴线的至少一个扫描轴的照明光和样品之间的相对运动用光栅显微镜观察至少一个样品区域的方法，其中与至少一个样品区域的平面成角度 相对运动，优选地与其垂直，第二扫描装置被移动，并且通过所耦合的第一和第二扫描装置的移动进行图像采集，并且通过样品的照射进行三维采样运动，其中第二打扫装置 耦合到第一扫描装置的运动，使得沿着第一扫描装置的至少一个扫描方向延伸的直线和/或曲线和/或平面和/或曲面被扫描，以及沿着 第二扫描装置的扫描方向。

公开(公告)号：US07649683B2

公开(公告)日：2010-01-19

申请号：US10967341

申请日：2004-10-19

申请人： Ralf Engelmann ,  Joerg-Michael Funk ,  Joerg Steinert ,  Bernhard Zimmermann ,  Stefan Wilhelm ,  Joerg Engel ,  Ulrich Meisel

发明人： Ralf Engelmann ,  Joerg-Michael Funk ,  Joerg Steinert ,  Bernhard Zimmermann ,  Stefan Wilhelm ,  Joerg Engel ,  Ulrich Meisel

IPC分类号： G02B21/06

CPC分类号： G02B21/008 ,  G02B21/0036 ,  Y10S359/90

摘要： Process for observing at least one sample region with a light raster microscope by a relative movement between the illumination light and sample via a first scanner along at least one scanning axis essentially perpendicular to the illumination axis wherein several illuminated sample points lie on a line and are detected simultaneously with a spatially resolving detector. At an angle to the plane of the relative movement, a second scanner is moved and an image acquisition takes place by coupling the movement of the first and second scanners and a three-dimensional sampling movement being done by the illumination of the sample. The second scanner is coupled to the movement of the first scanner such that straight and/or curved lines and/or plane and/or curved surfaces are scanned which are extended along at least one scanning direction of the first scanner as well as along the scanning direction of the second scanner.

摘要翻译： 使用光栅显微镜通过第一扫描仪沿着基本上垂直于照明轴的至少一个扫描轴的照射光和样本之间的相对运动来观察至少一个样本区域的过程，其中几个照亮的采样点位于一条线上，并且是 与空间分辨检测器同时检测。 与相对运动的平面成一角度，第二扫描器被移动，并且通过耦合第一和第二扫描器的运动以及通过样本的照明来完成三维采样运动来进行图像采集。 第二扫描器耦合到第一扫描仪的运动，使得扫描直线和/或曲线和/或平面和/或曲面，其沿着第一扫描器的至少一个扫描方向以及沿着扫描 第二扫描仪的方向。

公开(公告)号：US07271382B2

公开(公告)日：2007-09-18

申请号：US10967636

申请日：2004-10-19

申请人： Ralf Engelmann ,  Joerg-Michael Funk ,  Joerg Steinert ,  Bernhard Zimmermann ,  Stefan Wilhelm ,  Joerg Engel ,  Ulrich Meisel

发明人： Ralf Engelmann ,  Joerg-Michael Funk ,  Joerg Steinert ,  Bernhard Zimmermann ,  Stefan Wilhelm ,  Joerg Engel ,  Ulrich Meisel

IPC分类号： H01J3/14 ,  H01J5/16 ,  H01J40/14 ,  G06K9/00

CPC分类号： G02B21/002 ,  G02B21/0044 ,  G02B21/0064 ,  G02B21/008 ,  G02B21/248

摘要： Process for the observation of at least one sample region with a light raster microscope by a relative movement between the illumination light and sample via first scanning means along at least one scanning axis essentially perpendicular to the illumination axis wherein the illumination light illuminates the sample in parallel at several points or regions and several points or regions are detected simultaneously wherein at an angle to the plane of the relative movement, preferably perpendicular thereto, second scanning means are moved and an image acquisition takes place by the movement of the first and second scanning means being coupled and a three-dimensional sampling movement being done by the illumination of the sample wherein the second scanning means are coupled to the movement of the first scanning means in such a manner that straight and/or curved lines and/or plane and/or curved surfaces are scanned which are extended along at least one scanning direction of the first scanning means as well as along the scanning direction of the second scanning means.

摘要翻译： 用于通过第一扫描装置沿着基本上垂直于照明轴的至少一个扫描轴的照明光和样品之间的相对运动用光栅显微镜观察至少一个样本区域的过程，其中照明光平行照射样本 在多个点或区域处，同时检测多个点或区域，其中相对于相对运动的平面成一角度，优选垂直于其，第二扫描装置被移动，并且通过第一和第二扫描装置 被耦合，并且通过样品的照射来完成三维采样运动，其中第二扫描装置以这样的方式耦合到第一扫描装置的运动，使得直线和/或曲线和/或平面和/或 扫描沿着第一扫描线的至少一个扫描方向延伸的曲面 g装置以及沿着第二扫描装置的扫描方向。

公开(公告)号：US07459698B2

公开(公告)日：2008-12-02

申请号：US11590851

申请日：2006-11-01

申请人： Ralf Engelmann ,  Joerg-Michael Funk ,  Joerg Steinert ,  Bernhard Zimmermann ,  Stefan Wilhelm ,  Joerg Engel ,  Ulrich Meisel

发明人： Ralf Engelmann ,  Joerg-Michael Funk ,  Joerg Steinert ,  Bernhard Zimmermann ,  Stefan Wilhelm ,  Joerg Engel ,  Ulrich Meisel

IPC分类号： G01N21/64

CPC分类号： G02B21/0076 ,  G02B21/0048

摘要： Process for the observation of at least one sample region with a light raster microscope by a relative movement between the illumination light and sample via a first scanner along at least one scanning axis essentially perpendicular to the illumination axis wherein at an angle to the plane of the relative movement, preferably perpendicular thereto a second scanner is moved and an image acquisition takes place by the movement of the first and second scanners being coupled and a three-dimensional sampling movement being done by the illumination of the sample wherein the second scanner is coupled to the movement of the first scanner in such a manner that straight and/or curved lines and/or plane and/or curved surfaces are scanned which are extended along at least one scanning direction of the first scanner as well as along the scanning direction of the second scanner.

摘要翻译： 用于利用光栅显微镜通过第一扫描器沿着基本上垂直于照明轴的至少一个扫描轴的照明光和样本之间的相对运动来观察至少一个样本区域的过程，其中， 相对运动，优选地垂直于其，第二扫描器被移动，并且通过被耦合的第一和第二扫描器的运动而进行图像采集，并且三维采样运动是通过样本的照射进行的，其中第二扫描器耦合到 第一扫描器的运动以这样的方式扫描直线和/或曲线和/或平面和/或曲面，其沿着第一扫描仪的至少一个扫描方向以及沿着第一扫描仪的扫描方向延伸 第二台扫描仪

公开(公告)号：US20060011824A1

公开(公告)日：2006-01-19

申请号：US10967636

申请日：2004-10-19

申请人： Ralf Engelmann ,  Joerg-Michael Funk ,  Joerg Steinert ,  Bernhard Zimmermann ,  Stefan Wilhelm ,  Joerg Engel ,  Ulrich Meisel

发明人： Ralf Engelmann ,  Joerg-Michael Funk ,  Joerg Steinert ,  Bernhard Zimmermann ,  Stefan Wilhelm ,  Joerg Engel ,  Ulrich Meisel

IPC分类号： H01J40/14 ,  H01J7/24

CPC分类号： G02B21/002 ,  G02B21/0044 ,  G02B21/0064 ,  G02B21/008 ,  G02B21/248

摘要： Process for the observation of at least one sample region with a light raster microscope by a relative movement between the illumination light and sample via first scanning means along at least one scanning axis essentially perpendicular to the illumination axis wherein the illumination light illuminates the sample in parallel at several points or regions and several points or regions are detected simultaneously wherein at an angle to the plane of the relative movement, preferably perpendicular thereto, second scanning means are moved and an image acquisition takes place by the movement of the first and second scanning means being coupled and a three-dimensional sampling movement being done by the illumination of the sample wherein the second scanning means are coupled to the movement of the first scanning means in such a manner that straight and/or curved lines and/or plane and/or curved surfaces are scanned which are extended along at least one scanning direction of the first scanning means as well as along the scanning direction of the second scanning means.

摘要翻译： 用于通过第一扫描装置沿着基本上垂直于照明轴的至少一个扫描轴的照明光和样品之间的相对运动用光栅显微镜观察至少一个样本区域的过程，其中照明光平行照射样本 在多个点或区域处，同时检测多个点或区域，其中相对于相对运动的平面成一角度，优选垂直于其，第二扫描装置被移动，并且通过第一和第二扫描装置 被耦合，并且通过样品的照射来完成三维采样运动，其中第二扫描装置以这样的方式耦合到第一扫描装置的运动，使得直线和/或曲线和/或平面和/或 扫描沿着第一扫描线的至少一个扫描方向延伸的曲面 g装置以及沿着第二扫描装置的扫描方向。

公开(公告)号：US20060011804A1

公开(公告)日：2006-01-19

申请号：US10967341

申请日：2004-10-19

申请人： Ralf Engelmann ,  Joerg-Michael Funk ,  Joerg Steinert ,  Bernhard Zimmermann ,  Stefan Wilhelm ,  Joerg Engel ,  Ulrich Meisel

发明人： Ralf Engelmann ,  Joerg-Michael Funk ,  Joerg Steinert ,  Bernhard Zimmermann ,  Stefan Wilhelm ,  Joerg Engel ,  Ulrich Meisel

IPC分类号： G02B27/40

CPC分类号： G02B21/008 ,  G02B21/0036 ,  Y10S359/90

摘要： Process for the observation of at least one sample region with a light raster microscope by a relative movement between the illumination light and sample via first scanning means along at least one scanning axis essentially perpendicular to the illumination axis wherein several illuminated sample points lie on a line and are detected simultaneously with a spatially resolving detector wherein at an angle to the plane of the relative movement, preferably perpendicular thereto, second scanning means are moved and an image acquisition takes place by the movement of the first and second scanning means being coupled and a three-dimensional sampling movement being done by the illumination of the sample wherein the second scanning means are coupled to the movement of the first scanning means in such a manner that straight and/or curved lines and/or plane and/or curved surfaces are scanned which are extended along at least one scanning direction of the first scanning means as well as along the scanning direction of the second scanning means.

摘要翻译： 用于通过第一扫描装置沿着基本上垂直于照明轴的至少一个扫描轴的照明光和样品之间的相对运动用光栅显微镜观察至少一个样品区的方法，其中几个照亮的样品点位于一条线上 并且与空间分辨检测器同时检测器，其中相对于相对运动的平面，优选地与其垂直的平面移动第二扫描装置，并且通过耦合的第一和第二扫描装置的运动和 三维采样运动通过样品的照射来完成，其中第二扫描装置以扫描直线和/或曲线和/或平面和/或曲面扫描的方式与第一扫描装置的运动耦合 其沿着第一扫描装置的至少一个扫描方向以及沿着第一扫描装置的至少一个扫描方向延伸 第二扫描装置的扫描方向。

公开(公告)号：US08013288B2

公开(公告)日：2011-09-06

申请号：US12823242

申请日：2010-06-25

申请人： Joerg Steinert ,  Joerg Engel

发明人： Joerg Steinert ,  Joerg Engel

IPC分类号： H01J3/14

CPC分类号： G02B21/0032 ,  G02B21/0036

摘要： In a method for correcting a control of an optical scanner (14) which has a beam deflecting element (31) for deflecting a beam of optical radiation and a drive unit (30, 30′) for moving the beam deflecting element (31), said drive unit deflecting a beam of optical radiation directed at the beam deflecting element (31) according to a predetermined target movement using at least one parameter and/or a transfer function, preferably optical, said parameter or transfer function being used to control or regulate the system. In a determination step at least one current value of a drive unit transfer function that reproduces the response of the drive unit (30, 30′) to a predetermined target movement or a change in a target movement is ascertained for at least one frequency, and in a correction step at least one parameter and/or the transfer function is corrected as a function of the current value of the drive unit transfer function.

摘要翻译： 在用于校正光学扫描器（14）的控制的方法中，所述光学扫描器具有用于偏转光束的光束偏转元件（31）和用于移动光束偏转元件（31）的驱动单元（30,30'）， 所述驱动单元使用至少一个参数和/或传递函数（优选光学），使用用于控制或调节的参数或传递函数，根据预定的目标移动来偏转指向光束偏转元件（31）的光辐射束 系统。 在确定步骤中，确定至少一个频率的再现驱动单元（30,30'）对预定目标移动或目标移动变化的响应的驱动单元传递函数的至少一个当前值，以及 在校正步骤中，根据驱动单元传递函数的当前值来校正至少一个参数和/或传递函数。

公开(公告)号：US20070086048A1

公开(公告)日：2007-04-19

申请号：US11525209

申请日：2006-09-22

申请人： Joerg Steinert ,  Joerg Engel

发明人： Joerg Steinert ,  Joerg Engel

IPC分类号： G06F3/12

CPC分类号： G02B21/0032 ,  G02B21/0036

摘要： In a method for correcting a control of an optical scanner (14) which has a beam deflecting element (31) for deflecting a beam of optical radiation and a drive unit (30, 30′) for moving the beam deflecting element (31), said drive unit deflecting a beam of optical radiation directed at the beam deflecting element (31) according to a predetermined setpoint movement using at least one parameter and/or a transfer function, preferably optical, said parameter or transfer function being used to control or regulate the system. In a determination step at least one instantaneous value of a drive unit transfer function that reproduces the response of the drive unit (30, 30′) to a predetermined setpoint movement or a change in a setpoint movement is ascertained for at least one frequency, and in a correction step at least one parameter and/or the transfer function is corrected as a function of the instantaneous value of the drive unit transfer function.

摘要翻译： 在用于校正光学扫描器（14）的控制的方法中，所述光学扫描器具有用于偏转光束的光束偏转元件（31）和用于移动光束偏转元件（31）的驱动单元（30,30'）， 所述驱动单元使用至少一个参数和/或传递函数（最好是光学的），使用所述参数或传递函数来控制或调节用于根据预定的设定点移动来指向光束偏转元件（31）的光辐射束 系统。 在确定步骤中，确定至少一个频率的驱动单元传递函数的至少一个瞬时值，其将驱动单元（30,30'）的响应再现到预定的设定点移动或设定点移动的变化，并且 在校正步骤中，作为驱动单元传递函数的瞬时值的函数校正至少一个参数和/或传递函数。

公开(公告)号：US20050058372A1

公开(公告)日：2005-03-17

申请号：US10888123

申请日：2004-07-09

申请人： Ralf Engelmann ,  Joerg Engel

发明人： Ralf Engelmann ,  Joerg Engel

IPC分类号： G02B21/06 ,  G02B21/00 ,  G02B21/36 ,  G06K7/00 ,  H01S3/10

CPC分类号： G02B21/367 ,  G02B21/002

摘要： A method for the operation of a laser scanning microscope and for the detection of a specimen, wherein at least two detected images or image areas are compared to one another and temporal and/or spatial changes in the color and/or intensity lead to the formation of control signals for the illumination and/or detection and/or scanner control and/or other adjustable microscope components, and a laser scanning microscope with detection structure for detecting spatial and/or temporal changes in a specimen and/or in a specimen area and a control element for controlling the illumination and/or detection and/or scanner control and/or other optical and/or electronic microscope components depending on the detected change.

摘要翻译： 一种用于操作激光扫描显微镜和用于检测样本的方法，其中将至少两个检测到的图像或图像区域彼此进行比较，并且颜色和/或强度的时间和/或空间变化导致形成 用于照明和/或检测和/或扫描仪控制和/或其他可调节显微镜组件的控制信号，以及具有用于检测样本和/或样本区域中的空间和/或时间变化的检测结构的激光扫描显微镜， 用于根据检测到的变化来控制照明和/或检测和/或扫描仪控制和/或其他光学和/或电子显微镜部件的控制元件。