公开(公告)号：US09243261B2

公开(公告)日：2016-01-26

申请号：US13919300

申请日：2013-06-17

申请人： Ralph S. Quatrano ,  Yoichi Sakata ,  Izumi Yotsui

发明人： Ralph S. Quatrano ,  Yoichi Sakata ,  Izumi Yotsui

IPC分类号： C12N15/82 ,  C07K14/415

CPC分类号： C12N15/8273 ,  C07K14/415 ,  C12N15/8234 ,  C12N15/8293

摘要： A transgenic plant engineered to have increased desiccation tolerance, increased drought tolerance or increased water use efficiency, the plant transformed with an artificial DNA construct comprising a transcribable nucleic acid molecule encoding a polypeptide requiring both ABA and ABI3 to influence desiccation tolerance. Also provided are DNA constructs and methods of producing a transgenic plant engineered to have increased desiccation tolerance, increased drought tolerance or increased water use efficiency.

摘要翻译： 工程改造为具有增加的干燥耐受性，增加的耐旱性或增加的水利用效率的转基因植物，所述植物用包含编码需要ABA和ABI3两者以影响干燥耐受性的多肽的可转录核酸分子的人造DNA构建体转化。 还提供了DNA构建体和产生转基因植物的方法，所述转基因植物被工程化以具有增加的干燥耐受性，增加的耐旱性或增加的水利用效率。

公开(公告)号：US20140020132A1

公开(公告)日：2014-01-16

申请号：US13919300

申请日：2013-06-17

申请人： Ralph S. Quatrano ,  Yoichi Sakata ,  Izumi Yotsui

发明人： Ralph S. Quatrano ,  Yoichi Sakata ,  Izumi Yotsui

IPC分类号： C12N15/82

CPC分类号： C12N15/8273 ,  C07K14/415 ,  C12N15/8234 ,  C12N15/8293

摘要： A transgenic plant engineered to have increased desiccation tolerance, increased drought tolerance or increased water use efficiency, the plant transformed with an artificial DNA construct comprising a transcribable nucleic acid molecule encoding a polypeptide requiring both ABA and ABI3 to influence desiccation tolerance. Also provided are DNA constructs and methods of producing a transgenic plant engineered to have increased desiccation tolerance, increased drought tolerance or increased water use efficiency.

摘要翻译： 工程改造为具有增加的干燥耐受性，增加的耐旱性或增加的水利用效率的转基因植物，所述植物用包含编码需要ABA和ABI3两者以影响干燥耐受性的多肽的可转录核酸分子的人造DNA构建体转化。 还提供了DNA构建体和产生转基因植物的方法，所述转基因植物被工程化以具有增加的干燥耐受性，增加的耐旱性或增加的水利用效率。

公开(公告)号：US5139954A

公开(公告)日：1992-08-18

申请号：US525060

申请日：1990-05-18

申请人： James C. Litts ,  William R. Marcotte, Jr. ,  Ralph S. Quatrano

发明人： James C. Litts ,  William R. Marcotte, Jr. ,  Ralph S. Quatrano

IPC分类号： C04B35/465 ,  C07K14/415 ,  C12N15/82 ,  G11B5/10

CPC分类号： C07K14/415 ,  C04B35/465 ,  C12N15/8234 ,  C12N15/8238 ,  C12N15/8273 ,  G11B5/10

摘要： The preparation and use of nucleic acid promoter fragments homologous to the Em gene of wheat to bring the expression of selected genes in plants under external control are described. The Em promoter fragment is responsive to abscisic acid (ABA) and other compounds possessing ABA-like activity. Through transformation of protoplasts and plant cells with recombinant DNA constructs incorporating such promoter fragments, operably linked selected genes are expressed in response to ABA and compounds possessing ABA-like activity. The application of such promoter fragments and constructs to transient assay systems to predict the likelihood of stable transformation in plants is disclosed.

摘要翻译： 描述了与小麦的Em基因同源的核酸启动子片段的制备和使用，以在外部控制下使所选择的基因在植物中的表达。 Em启动子片段对脱落酸（ABA）和具有ABA样活性的其它化合物有反应。 通过用包含这种启动子片段的重组DNA构建体转化原生质体和植物细胞，可操作地连接的选择的基因是响应于ABA和具有ABA样活性的化合物而表达的。 公开了将这种启动子片段和构建体应用于瞬时测定系统以预测植物中稳定转化的可能性。