-
公开(公告)号:US20120157350A1
公开(公告)日:2012-06-21
申请号:US13330535
申请日:2011-12-19
申请人: Randall J. True , Jared C. Pache
发明人: Randall J. True , Jared C. Pache
IPC分类号: C40B60/12
CPC分类号: B82Y25/00 , B82Y20/00 , G01N33/585 , G09F3/00 , H01F1/0072
摘要: Presented are encoded microparticles, methods of use in biological assays, and flexible, modular instrument systems for conducting a variety of biological assays using the encoded microparticles. The systems include various instrumentation components which are exchangeable and offer a single flexible platform on which a large number of different types of experiments and assays may be conducted including, for example, microarray analysis, encoded microparticle single and multiplex detection, tissue dissection and isolation, and other genetic analysis techniques.
摘要翻译: 呈现的是编码微粒,生物测定中使用的方法,以及灵活的模块化仪器系统,用于使用编码的微粒进行各种生物测定。 这些系统包括可交换的各种仪器组件,并提供单个柔性平台,在该平台上可以进行大量不同类型的实验和测定,包括例如微阵列分析,编码的微粒单次和多重检测,组织解剖和分离, 和其他遗传分析技术。
-
公开(公告)号:US20120231453A1
公开(公告)日:2012-09-13
申请号:US13336699
申请日:2011-12-23
申请人: Randall J. True , Yunqing Ma , Aiguo Zhang , Franklin R. Witney
发明人: Randall J. True , Yunqing Ma , Aiguo Zhang , Franklin R. Witney
IPC分类号: C12Q1/68
CPC分类号: B82Y25/00 , B82Y20/00 , G09F3/00 , H01F1/0072
摘要: An encoded microparticle carrying a spatial code is provided; and a set of encoded microparticles are provided with distinguishable spatial codes, wherein the codes comply with a pre-determined coding scheme. Presented are also methods of using the encoded microparticles in various biological assays, such as various multiplex quantitative PCR (real-time PCR) and multiplex chromosomal immunoprecipitation (ChIP) assays.
摘要翻译: 提供携带空间码的编码微粒; 并且一组编码的微粒被提供有可区分的空间代码,其中代码符合预定的编码方案。 还提出了在各种生物测定中使用编码的微粒的方法,例如各种多重定量PCR(实时PCR)和多重染色体免疫沉淀(ChIP)测定。
-
公开(公告)号:US20100297448A1
公开(公告)日:2010-11-25
申请号:US12779413
申请日:2010-05-13
IPC分类号: B32B9/00
CPC分类号: G01N33/585 , B82Y25/00 , H01F10/10 , Y10T428/2991
摘要: A system and method for encoded microparticles is described. One embodiment includes an encoded microparticle comprising a plurality of segments, wherein the plurality of segments form a spatial code; contrast coating on at least one segment of the plurality of segments, wherein the contrast coating further encodes the microparticle; an outer surface, wherein the outer surface encloses the spatial code and contrast coating, and wherein the spatial code and contrast coating are detectable through the outer surface.
摘要翻译: 描述了编码微粒的系统和方法。 一个实施例包括包含多个段的编码微粒,其中多个段形成空间代码; 在多个片段的至少一个片段上形成对比涂层,其中所述造影剂进一步编码所述微粒; 外表面,其中所述外表面包围所述空间代码和对比涂层,并且其中所述空间代码和对比涂层可通过所述外表面检测。
-
公开(公告)号:US08883691B2
公开(公告)日:2014-11-11
申请号:US12215607
申请日:2008-06-25
申请人: Randall J. True
发明人: Randall J. True
IPC分类号: C40B30/04 , G01N33/543 , G01N21/64
CPC分类号: G01N33/54313 , B01J2219/005 , B01J2219/00502 , B01J2219/00513 , B01J2219/00527 , B01J2219/00547 , B01J2219/00549 , B01J2219/00558 , B01J2219/0056 , G01N33/54366 , G01N2021/6441 , Y10T428/2982 , Y10T428/2991
摘要: Microparticles including spatially coded microparticles, systems for imaging and methods of detecting such microparticles as well as using the same in bioassays are provided.
摘要翻译: 提供了包括空间编码的微粒的微粒,用于成像的系统和检测这种微粒的方法以及在生物测定中使用微粒。
-
公开(公告)号:US08592136B2
公开(公告)日:2013-11-26
申请号:US11521058
申请日:2006-09-13
申请人: Randall J. True
发明人: Randall J. True
IPC分类号: G03F7/20
CPC分类号: G01N33/532 , B82Y25/00 , G01N33/585 , G03F7/0002 , G03F7/0035 , G09F3/00 , H01F1/0072 , Y10T428/2989 , Y10T428/2996 , Y10T436/143333
摘要: Methods and apparatus are provided for forming a plurality of encoded microparticles with a printing process to define a code for identifying the microparticles. In some embodiments the printing process includes printing steps performed with photolithography.
摘要翻译: 提供了用印刷方法形成多个编码的微粒的方法和装置,以定义用于识别微粒的代码。 在一些实施例中,印刷过程包括用光刻法执行的印刷步骤。
-
公开(公告)号:US20120196381A1
公开(公告)日:2012-08-02
申请号:US13337024
申请日:2011-12-23
CPC分类号: B82Y25/00 , B82Y20/00 , G01N33/54326 , G01N33/585 , G09F3/00 , G11B5/66 , H01F1/0072
摘要: An encoded microparticle having a spatial code is provided; and a set of encoded microparticles possessing subsets each provided with a distinguishable spatial code, wherein the codes comply with a pre-determined coding scheme. Presented are also methods of using the encoded microparticles in various biological assays, such as various multiplex assays and visualizing them by creating a digital image of the encoded microparticles and determining whether false positives are present. Further are provided methods of manufacture of the encoded microparticles which employ ferromagnetic nanoparticles applied using spin-on-glass techniques.
摘要翻译: 提供具有空间码的编码微粒; 以及一组编码的微粒,其具有各自具有可区分的空间码的子集,其中所述码符合预定编码方案。 还提出了在各种生物测定中使用编码的微粒的方法,例如各种多重测定,并通过产生编码的微粒的数字图像并确定是否存在假阳性来使其可视化。 还提供了使用旋涂玻璃技术应用铁磁性纳米粒子的编码微粒的制造方法。
-
公开(公告)号:US08697334B2
公开(公告)日:2014-04-15
申请号:US13337024
申请日:2011-12-23
CPC分类号: B82Y25/00 , B82Y20/00 , G01N33/54326 , G01N33/585 , G09F3/00 , G11B5/66 , H01F1/0072
摘要: An encoded microparticle having a spatial code is provided; and a set of encoded microparticles possessing subsets each provided with a distinguishable spatial code, wherein the codes comply with a pre-determined coding scheme. Presented are also methods of using the encoded microparticles in various biological assays, such as various multiplex assays and visualizing them by creating a digital image of the encoded microparticles and determining whether false positives are present. Further are provided methods of manufacture of the encoded microparticles which employ ferromagnetic nanoparticles applied using spin-on-glass techniques.
摘要翻译: 提供具有空间码的编码微粒; 以及一组编码的微粒,其具有各自具有可区分的空间码的子集,其中所述码符合预定编码方案。 还提出了在各种生物测定中使用编码的微粒的方法,例如各种多重测定,并通过产生编码的微粒的数字图像并确定是否存在假阳性来使其可视化。 还提供了使用旋涂玻璃技术应用铁磁性纳米粒子的编码微粒的制造方法。
-
公开(公告)号:US08460854B2
公开(公告)日:2013-06-11
申请号:US13340254
申请日:2011-12-29
申请人: Randall J. True
发明人: Randall J. True
CPC分类号: G01N33/532 , B82Y25/00 , G01N33/585 , G03F7/0002 , G03F7/0035 , G09F3/00 , H01F1/0072 , Y10T428/2989 , Y10T428/2996 , Y10T436/143333
摘要: A system and method for forming encoded microparticles is described. One embodiment includes a method for forming a microparticle, the method comprising providing a pattern, wherein the pattern defines a code element, printing the pattern on a substrate to form a first code element within a microparticle region, printing the pattern on the substrate to form at least one successive code element, such that the first code element and the at least one successive code element are within the same microparticle region, wherein a code is formed by the first code element and any successive code elements.
摘要翻译: 描述了用于形成编码的微粒的系统和方法。 一个实施例包括形成微粒的方法,所述方法包括提供图案,其中所述图案限定代码元素,将图案印刷在基底上以在微粒区域内形成第一代码元素,将图案印刷在基底上以形成 至少一个连续的代码元素,使得第一代码元素和至少一个连续代码元素在相同的微粒区域内,其中代码由第一代码元素和任何连续代码元素形成。
-
公开(公告)号:US08178278B2
公开(公告)日:2012-05-15
申请号:US12779413
申请日:2010-05-13
CPC分类号: G01N33/585 , B82Y25/00 , H01F10/10 , Y10T428/2991
摘要: A system and method for encoded microparticles is described. One embodiment includes an encoded microparticle comprising a plurality of segments, wherein the plurality of segments form a spatial code; contrast coating on at least one segment of the plurality of segments, wherein the contrast coating further encodes the microparticle; an outer surface, wherein the outer surface encloses the spatial code and contrast coating, and wherein the spatial code and contrast coating are detectable through the outer surface.
摘要翻译: 描述了编码微粒的系统和方法。 一个实施例包括包含多个段的编码微粒,其中多个段形成空间代码; 在多个片段的至少一个片段上形成对比涂层,其中所述造影剂进一步编码所述微粒; 外表面,其中所述外表面包围所述空间代码和对比涂层,并且其中所述空间代码和对比涂层可通过所述外表面检测。
-
公开(公告)号:US20100227279A1
公开(公告)日:2010-09-09
申请号:US11521058
申请日:2006-09-13
申请人: Randall J. True
发明人: Randall J. True
CPC分类号: G01N33/532 , B82Y25/00 , G01N33/585 , G03F7/0002 , G03F7/0035 , G09F3/00 , H01F1/0072 , Y10T428/2989 , Y10T428/2996 , Y10T436/143333
摘要: Methods and apparatus are provided for forming a plurality of encoded microparticles with a printing process to define a code for identifying the microparticles. In some embodiments the printing process includes printing steps performed with photolithography.
摘要翻译: 提供了用印刷方法形成多个编码的微粒的方法和装置,以定义用于识别微粒的代码。 在一些实施例中,印刷过程包括用光刻法执行的印刷步骤。
-
-
-
-
-
-
-
-
-