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公开(公告)号：US5418162A
公开(公告)日：1995-05-23
申请号：US959943
申请日：1992-10-14
CPC分类号： C07K14/47 , C12Q1/6876
摘要： Isolated DNA encoding a serotonin transporter is disclosed. Also disclosed are vectors and host cells containing the aforesaid DNA, methods of using the same, purified protein by the same, and oligonucleotides and antibodies which bind thereto. Specific embodiments are cDNAs encoding rat and human serotonin transporter.
摘要翻译： 公开了编码5-羟色胺转运蛋白的分离的DNA。 还公开了含有上述DNA的载体和宿主细胞，使用它们的方法，其纯化的蛋白质以及与其结合的寡核苷酸和抗体。 具体实施方案是编码大鼠和人5-羟色胺转运蛋白的cDNA。
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2.发明授权High affinity L-proline transporter polypeptides; antibodies and immunoassays specific for them 失效高亲和力L-脯氨酸转运蛋白多肽; 针对他们的抗体和免疫测定
公开(公告)号：US5759788A
公开(公告)日：1998-06-02
申请号：US753985
申请日：1996-12-03
IPC分类号： A61K38/00 , C07K14/47 , C07K14/705 , C12N15/12 , G01N33/53
CPC分类号： C07K14/47 , C07K14/705 , A61K38/00
摘要： The anatomical distribution, nucleic acid sequence, pharmacological properties, and inferred structural features of a cDNA encoding a high affinity, Na.sup.+ -dependent rat brain L-proline transporter is described. The expression of this carrier in subpopulations of putative glutamatergic pathways supports a specific role for L-proline in excitatory amino acid neurotransmission. The cloned transporter cDNA predicts a 637 amino acid protein with 12 putative transmembrane domains and exhibits 44%-45% amino acid sequence identity with other neurotransmitter transporters. These findings support a synaptic role for L-proline in specific excitatory pathways in the CNS. The sequence can be used for expression of the transporter molecule, to make probes for the same protein from other species and related proteins, in diagnostic assays, and to design functional and structural analogs for use in research and possible clinical treatments. The protein is useful in making antibodies, conducting research studies, and design of therapeutic transporter modulators for clinical treatments.
摘要翻译： 描述了编码高亲和力，Na +依赖性大鼠脑L-脯氨酸转运蛋白的cDNA的解剖分布，核酸序列，药理学性质和推断的结构特征。 该载体在推定的谷氨酸能途径的亚群中的表达支持L-脯氨酸在兴奋性氨基酸神经传递中的特异性作用。 克隆的转运蛋白cDNA预测具有12个推定的跨膜结构域的637个氨基酸的蛋白质，并且与其他神经递质转运体显示44％-45％的氨基酸序列同一性。 这些发现支持L-脯氨酸在CNS中特异性兴奋途径中的突触作用。 该序列可用于表达转运蛋白分子，在诊断测定中制备来自其他物种和相关蛋白质的相同蛋白质的探针，并设计用于研究和可能的临床治疗的功能和结构类似物。 该蛋白质可用于制备抗体，进行研究和设计用于临床治疗的治疗性转运蛋白调节剂。
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3.发明授权High affinity, brain-specific nucleic acids encoding a L-proline transporter, and vectors, and host cells comprising the same 失效编码L-脯氨酸转运蛋白的高亲和性，脑特异性核酸，和载体，以及包含其的宿主细胞
公开(公告)号：US5580775A
公开(公告)日：1996-12-03
申请号：US879617
申请日：1992-05-01
CPC分类号： C07K14/47 , C07K14/705 , A61K38/00
摘要： The anatomical distribution, nucleic acid sequence, pharmacological properties, and inferred structural features of a cDNA encoding a high affinity, Na.sup.+ -dependent rat brain L-proline transporter is described. The expression of this carrier in subpopulations of putative glutamatergic pathways supports a specific role for L-proline in excitatory amino acid neurotransmission. The cloned transporter cDNA predicts a 637 amino acid protein with 12 putative transmembrane domains and exhibits 44%-45% amino acid sequence identity with other neurotransmitter transporters. These findings support a synaptic role for L-proline in specific excitatory pathways in the CNS. The sequence can be used for expression of the transporter molecule, to make probes for the same protein from other species and related proteins, in diagnostic assays, and to design functional and structural analogs for use in research and possible clinical treatments. The protein is useful in making antibodies, conducting research studies, and design of therapeutic transporter modulators for clinical treatments.
摘要翻译： 描述了编码高亲和力，Na +依赖性大鼠脑L-脯氨酸转运蛋白的cDNA的解剖分布，核酸序列，药理学性质和推断的结构特征。 该载体在推定的谷氨酸能途径的亚群中的表达支持L-脯氨酸在兴奋性氨基酸神经传递中的特异性作用。 克隆的转运蛋白cDNA预测具有12个推定的跨膜结构域的637个氨基酸的蛋白质，并且与其他神经递质转运体显示44％-45％的氨基酸序列同一性。 这些发现支持L-脯氨酸在CNS的特异性兴奋途径中的突触作用。 该序列可用于表达转运蛋白分子，在诊断测定中制备来自其他物种和相关蛋白质的相同蛋白质的探针，并设计用于研究和可能的临床治疗的功能和结构类似物。 该蛋白质可用于制备抗体，进行研究和设计用于临床治疗的治疗性转运蛋白调节剂。
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公开(公告)号：US06818391B2
公开(公告)日：2004-11-16
申请号：US09915181
申请日：2001-07-24
IPC分类号： C12Q100
CPC分类号： C07K14/47 , A01K2217/075 , G01N2333/70571
摘要： This invention pertains to the identification of a novel class of glutamate transporters. In particular, this invention pertains to the discovery that proteins originally considered to perform an entirely different function (BNPI, DNPI, etc.), in fact, transport glutamate into synaptic vesicles. Designated VGLUT glutamate transporters, the transporters provide good targets with which to screen for modulators of glutamate uptake into synaptic vesicles.
摘要翻译： 本发明涉及一类新型谷氨酸转运蛋白的鉴定。 特别地，本发明涉及以下发现：实际上，原本被认为是完全不同的功能（BNPI，DNPI等）的蛋白质将谷氨酸转运到突触小泡中。 指定的VGLUT谷氨酸转运蛋白，转运蛋白提供了良好的目标，筛选谷氨酸摄取调节剂到突触小泡。
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