-
公开(公告)号：US08647827B2
公开(公告)日：2014-02-11
申请号：US13111713
申请日：2011-05-19
IPC分类号： G01N33/53 , G01N33/567 , C12Q1/02
CPC分类号： G01N33/9406 , G01N33/502 , G01N33/6872
摘要： The invention is based on the finding that IDT307 and analogs thereof are fluorescent substrates transported by several neurotransmitter transporters. Provided are methods for the analysis of neurotransmitter transport and binding using IDT307 and its analogs. The invention also provides rapid methods for screening for modulators of neurotransmitter transport.
摘要翻译： 本发明基于IDT307及其类似物是由几种神经递质转运蛋白转运的荧光底物的发现。 提供了使用IDT307及其类似物分析神经递质转运和结合的方法。 本发明还提供用于筛选神经递质转运调节剂的快速方法。
-
公开(公告)号：US20110229910A1
公开(公告)日：2011-09-22
申请号：US13111713
申请日：2011-05-19
CPC分类号： G01N33/9406 , G01N33/502 , G01N33/6872
摘要： The invention is based on the finding that IDT307 and analogs thereof are fluorescent substrates transported by several neurotransmitter transporters. Provided are methods for the analysis of neurotransmitter transport and binding using IDT307 and its analogs. The invention also provides rapid methods for screening for modulators of neurotransmitter transport.
摘要翻译： 本发明基于IDT307及其类似物是由几种神经递质转运蛋白转运的荧光底物的发现。 提供了使用IDT307及其类似物分析神经递质转运和结合的方法。 本发明还提供用于筛选神经递质转运调节剂的快速方法。
-
公开(公告)号：US07439039B2
公开(公告)日：2008-10-21
申请号：US10884194
申请日：2004-07-02
CPC分类号： C07K14/47
摘要： The serotonin transporter (SERT) is a target of various therapeutic agents used in the treatment of neurological and neurodegenerative disorders. The present invention provides novel forms of SERT that lacks high affinity recognition of specific serotonin reuptake inhibitors (SSRIs). The present invention therefore provides a novel target for use in screening and model development that can aid both the discovery of new medications and the discovery of novel pathways impacted in parallel with SERT blockade. Such novel targets can help identify new SSRI's with unique modifications and lead to discovery of pathways that secondarily support the therapeutic activity of these agents.
摘要翻译： 血清素转运蛋白（SERT）是用于治疗神经和神经退行性疾病的各种治疗剂的靶标。 本发明提供了对特异性5-羟色胺再摄取抑制剂（SSRI）缺乏高亲和力识别的SERT的新型形式。 因此，本发明提供了用于筛选和模型开发的新靶标，其可以帮助新药物的发现和发现与SERT阻断并行的新途径。 这种新颖的靶标可以帮助鉴定新的SSRI的独特修饰，并导致二次支持这些药物治疗活性的途径的发现。
-
4.发明授权公开(公告)号：US07531713B2
公开(公告)日：2009-05-12
申请号：US11029895
申请日：2005-01-05
申请人： Randy D. Blakely , Richard Nass , David Miller
发明人： Randy D. Blakely , Richard Nass , David Miller
CPC分类号： G01N33/5014 , A01K67/0336 , A01K2217/05 , A01K2227/703 , A01K2267/035 , A01K2267/0393 , C12N2830/008 , G01N33/5008 , G01N33/5058 , G01N2500/00
摘要： Provided are in vivo screening methods to detect and identify substances that affect neuronal viability, and/or prevent neurodegeneration, and/or confer neuroprotective effects The screening methods utilize recombinant C. elegans expressing a detectable marker in neuronal sub-groups and the use of neurotoxins specific to specific neuronal cells. Also provided are methods for identifying modulators of neurotransmitter transporters such as the dopamine transporter. Therefore, the invention provides methods for identifying substances that can be used in the prevention and therapy of neurodegenerative diseases.
摘要翻译： 提供了用于检测和鉴定影响神经元存活力和/或预防神经变性和/或赋予神经保护作用的物质的体内筛选方法。筛选方法利用在神经元亚组中表达可检测标记的重组秀丽隐杆线虫和神经毒素的使用 特异性的特定神经细胞。 还提供了用于鉴定神经递质转运蛋白如多巴胺转运蛋白的调节剂的方法。 因此，本发明提供了用于鉴定可用于预防和治疗神经变性疾病的物质的方法。
-
公开(公告)号：US07338799B2
公开(公告)日：2008-03-04
申请号：US10724806
申请日：2003-12-01
CPC分类号： C07K14/47 , A01K2217/05 , A61K48/00
摘要： Human high affinity choline transporter (CHT) cDNA was cloned from spinal cord and the primary structure and chromosomal localization have been determined. Mouse high affinity CHT cDNA was also cloned and characterized. An isolated polynucleotide, an isolated polypeptide, a recombinant host cell, a recombinant vector, a purified protein, an antibody, a nucleic acid detection kit, a method for screening cholinergic therapeutics, a method of treating a patient, a method of gene therapy and transgenic CHT mice are discussed.
摘要翻译： 从脊髓中克隆人高亲和力胆碱转运蛋白（CHT）cDNA，并确定了一级结构和染色体定位。 还克隆和表征小鼠高亲和力的CHT cDNA。 分离的多核苷酸，分离的多肽，重组宿主细胞，重组载体，纯化蛋白，抗体，核酸检测试剂盒，用于筛选胆碱能治疗剂的方法，治疗患者的方法，基因治疗方法和 讨论了转基因CHT小鼠。
-
公开(公告)号：US07318917B2
公开(公告)日：2008-01-15
申请号：US10656897
申请日：2003-09-05
IPC分类号： A61K49/00 , A61K38/43 , C07D487/00 , C12N9/00 , C07K14/01
CPC分类号： G01N33/9406
摘要： The invention describes the finding that 4-(4-dimethylaminostyrl)-N-methylpyridinium or ASP+ is a fluorescent substrate that is transported by several neurotransmitter transporters. Provided are methods for the analysis of neurotransmitter transport and binding using ASP+. The invention also provides rapid methods for screening for modulators of neurotransmitter transport. As neurotransmitter transporter defects are associated with numerous neurological disorders, the invention also provides methods for treating neurotransmitter transport-associated defects/conditions using the modulators identified by the screening methods of the invention.
摘要翻译： 本发明描述了4-（4-二甲基氨基苯甲酰基）-N-甲基吡啶或ASP + +是通过几种神经递质转运蛋白转运的荧光底物的发现。 提供了使用ASP＆lt; SUP＆gt;分析神经递质转运和结合的方法。 本发明还提供用于筛选神经递质转运调节剂的快速方法。 由于神经递质转运蛋白缺陷与许多神经障碍有关，本发明还提供了使用通过本发明的筛选方法鉴定的调节剂治疗神经递质转运相关缺陷/病症的方法。
-
7.发明授权公开(公告)号：US06894205B2
公开(公告)日：2005-05-17
申请号：US09888233
申请日：2001-06-22
申请人： Randy D. Blakely , Richard Nass , David Miller
发明人： Randy D. Blakely , Richard Nass , David Miller
IPC分类号： A01K67/033 , C12N5/02 , G01N33/50 , G01N33/00 , A01K67/00
CPC分类号： G01N33/5014 , A01K67/0336 , A01K2217/05 , A01K2227/703 , A01K2267/035 , A01K2267/0393 , C12N2830/008 , G01N33/5008 , G01N33/5058 , G01N2500/00
摘要： Provided are in vivo screening methods to detect and identify substances that affect neuronal viability, and/or prevent neurodegeneration, and/or confer neuroprotective effects. The screening methods utilize recombinant C. elegans expressing a detectable marker in neuronal sub-groups and the use of neurotoxins specific to specific neuronal cells. Also provided are methods for identifying modulators of neurotransmitter transporters such as the dopamine transporter. Therefore, the invention provides methods for identifying substances that can be used in the prevention and therapy of neurodegenerative diseases.
摘要翻译： 提供了体内筛选方法来检测和鉴定影响神经元活力，和/或预防神经变性和/或赋予神经保护作用的物质。 筛选方法利用在神经元亚组中表达可检测标记的重组秀丽隐杆线虫和使用特异性神经元细胞的神经毒素。 还提供了用于鉴定神经递质转运蛋白如多巴胺转运蛋白的调节剂的方法。 因此，本发明提供了用于鉴定可用于预防和治疗神经变性疾病的物质的方法。
-
8.发明授权Nucleic acid encoding Nematode dopamine transporter and the protein encoded thereby 失效核酸编码线虫多巴胺转运蛋白和由此编码的蛋白质公开(公告)号：US06596512B1
公开(公告)日：2003-07-22
申请号：US09530157
申请日：2000-07-18
申请人： Randy D. Blakely , Cecil M. Eppler
发明人： Randy D. Blakely , Cecil M. Eppler
IPC分类号： C07K14705
CPC分类号： G01N33/6872 , C07K14/43545 , G01N33/5008 , G01N33/56905 , G01N33/9413 , G01N2333/4353
摘要： A nematode dopamine tansporter cDNA has been cloned, sequenced, and expressed, and provides a convenient screening assay for antiparasitics, as well as psychoactive drugs.
摘要翻译： 已经克隆，测序和表达了一种线虫多巴胺tansporter cDNA，并提供了一种方便的抗寄生虫筛选方法以及精神活性药物。
-
公开(公告)号：US5418162A
公开(公告)日：1995-05-23
申请号：US959943
申请日：1992-10-14
CPC分类号： C07K14/47 , C12Q1/6876
摘要： Isolated DNA encoding a serotonin transporter is disclosed. Also disclosed are vectors and host cells containing the aforesaid DNA, methods of using the same, purified protein by the same, and oligonucleotides and antibodies which bind thereto. Specific embodiments are cDNAs encoding rat and human serotonin transporter.
摘要翻译： 公开了编码5-羟色胺转运蛋白的分离的DNA。 还公开了含有上述DNA的载体和宿主细胞，使用它们的方法，其纯化的蛋白质以及与其结合的寡核苷酸和抗体。 具体实施方案是编码大鼠和人5-羟色胺转运蛋白的cDNA。
-
公开(公告)号：US07947255B2
公开(公告)日：2011-05-24
申请号：US11832905
申请日：2007-08-02
CPC分类号： G01N33/9406 , G01N33/502 , G01N33/6872
摘要： The invention is based on the finding that IDT307 and analogs thereof are fluorescent substrates transported by several neurotransmitter transporters. Provided are methods for the analysis of neurotransmitter transport and binding using IDT307 and its analogs. The invention also provides rapid methods for screening for modulators of neurotransmitter transport.
摘要翻译： 本发明基于IDT307及其类似物是由几种神经递质转运蛋白转运的荧光底物的发现。 提供了使用IDT307及其类似物分析神经递质转运和结合的方法。 本发明还提供用于筛选神经递质转运调节剂的快速方法。
-
-
-
-
-
-
-
-
-
搜索结果
14 条记录 (耗时 0.034 秒)