公开(公告)号：US07994391B2

公开(公告)日：2011-08-09

申请号：US12018292

申请日：2008-01-23

申请人： Rebecca E. Cahoon ,  William James Gordon-Kamm ,  Yumin Tao ,  Keith S. Lowe ,  Christopher Jay Scelonge

发明人： Rebecca E. Cahoon ,  William James Gordon-Kamm ,  Yumin Tao ,  Keith S. Lowe ,  Christopher Jay Scelonge

IPC分类号： C12N15/29 ,  C12N15/82 ,  A01H5/00 ,  A01H5/10

CPC分类号： C12N15/8241 ,  C07K14/415 ,  C12N15/62 ,  C12N15/8201 ,  C12N15/8207 ,  C12N15/8261 ,  Y02A40/146

摘要： This invention relates to isolated polynucleotides encoding WUS polypeptides. The invention further provides isolated WUS polypeptides. The invention also provides methods of using the polynucleotides to modulate the level of WUS, improve transformation efficiency, to stimulate plant cell growth, including stem cells, to stimulate organogenesis, to stimulate somatic embryogenesis, to induce apomixis, and to provide a positive selection for cells comprising the polynucleotide. The invention also relates to cells, plants and seeds comprising the polynucleotides of the invention or produced by the methods of the invention.

摘要翻译： 本发明涉及编码WUS多肽的分离的多核苷酸。 本发明还提供了分离的WUS多肽。 本发明还提供了使用多核苷酸来调节WUS水平，提高转化效率，刺激植物细胞生长（包括干细胞）刺激器官发生，刺激体细胞胚发生，诱导无融合生殖以及提供阳性选择的方法 包含多核苷酸的细胞。 本发明还涉及包含本发明的多核苷酸或通过本发明的方法产生的细胞，植物和种子。

公开(公告)号：US08383891B2

公开(公告)日：2013-02-26

申请号：US13168133

申请日：2011-06-24

申请人： Rebecca E. Cahoon ,  William James Gordon-Kamm ,  Keith S. Lowe ,  Christopher Jay Scelonge ,  Yumin Tao

发明人： Rebecca E. Cahoon ,  William James Gordon-Kamm ,  Keith S. Lowe ,  Christopher Jay Scelonge ,  Yumin Tao

IPC分类号： C12N15/29 ,  C12N15/82 ,  A01H5/00 ,  A01H5/10

CPC分类号： C12N15/8241 ,  C07K14/415 ,  C12N15/62 ,  C12N15/8201 ,  C12N15/8207 ,  C12N15/8261 ,  Y02A40/146

摘要： This invention relates to isolated polynucleotides encoding WUS polypeptides. The invention further provides isolated WUS polypeptides. The invention also provides methods of using the polynucleotides to modulate the level of WUS, improve transformation efficiency, to stimulate plant cell growth, including stem cells, to stimulate organogenesis, to stimulate somatic embryogenesis, to induce apomixis, and to provide a positive selection for cells comprising the polynucleotide. The invention also relates to cells, plants and seeds comprising the polynucleotides of the invention or produced by the methods of the invention.

公开(公告)号：US07348468B1

公开(公告)日：2008-03-25

申请号：US09807946

申请日：2000-09-28

申请人： Rebecca E. Cahoon ,  Keith Lowe ,  William James Gordon-Kamm ,  Yumin Tao ,  Christopher Jay Scelonge

发明人： Rebecca E. Cahoon ,  Keith Lowe ,  William James Gordon-Kamm ,  Yumin Tao ,  Christopher Jay Scelonge

IPC分类号： C12N15/29 ,  C12N15/82 ,  A01H5/00 ,  A01H5/10

CPC分类号： C12N15/8241 ,  C07K14/415 ,  C12N15/62 ,  C12N15/8201 ,  C12N15/8207 ,  C12N15/8261 ,  Y02A40/146

摘要： This invention relates to an isolated nucleic acid fragment encoding a WUS protein. The invention also relates to the construction of a chimeric gene encoding all or a portion of the WUS protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the WUS protein in a transformed host cell.

摘要翻译： 本发明涉及编码WUS蛋白的分离的核酸片段。 本发明还涉及在正义或反义方向上构建编码WUS蛋白的全部或一部分的嵌合基因，其中嵌合基因的表达导致转化宿主细胞中WUS蛋白水平的改变。

公开(公告)号：US20140338070A1

公开(公告)日：2014-11-13

申请号：US14236597

申请日：2012-07-18

申请人： Andrew Mark Cigan ,  Saverio Carl Falco ,  Huirong Gao ,  Michael W. Lassner ,  Zhongsen Li ,  Zhan-Bin Liu ,  Leszek A. Lyznik ,  David J. Peterson ,  Christopher Jay Scelonge

发明人： Andrew Mark Cigan ,  Saverio Carl Falco ,  Huirong Gao ,  Michael W. Lassner ,  Zhongsen Li ,  Zhan-Bin Liu ,  Leszek A. Lyznik ,  David J. Peterson ,  Christopher Jay Scelonge

IPC分类号： C12N15/82

CPC分类号： C12N15/8213

摘要： Compositions and methods are provided for the targeted integration of a polynucleotide sequence of interest into the genome of a plant or plant cell. The methods and compositions employ recognition sites for endonucleases and endonucleases in combination with site-specific recombination sites/recombinases to provide an effective system for establishing target sites within the genome of a plant, plant cell or seed. Once such target sites are established, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest.

摘要翻译： 提供组合物和方法用于将目的多核苷酸序列靶向整合到植物或植物细胞的基因组中。 方法和组合物使用核酸内切酶和内切核酸酶的识别位点与位点特异性重组位点/重组酶组合，以提供用于在植物，植物细胞或种子的基因组内建立靶位点的有效系统。 一旦建立了这样的靶位点，就可以采用多种方法来进一步修饰靶位点，使得它们含有多种感兴趣的多核苷酸。

公开(公告)号：US06265638B1

公开(公告)日：2001-07-24

申请号：US09407574

申请日：1999-09-28

申请人： Dennis L. Bidney ,  Christopher Jay Scelonge

发明人： Dennis L. Bidney ,  Christopher Jay Scelonge

IPC分类号： C12N1582

CPC分类号： C12N15/8241 ,  C12N15/8205 ,  C12N15/8209

摘要： Compositions and methods for the efficient co-transformation of a plant are provided. Novel compositions are Agrobacterium strains that have been engineered to comprise at least two binary vector plasmids in addition to a helper plasmid comprising the vir functions. Each of the binary vectors comprises its own T-DNA borders flanking a heterologous nucleotide sequence of interest. Methods of the invention comprise the use of these novel multiple-binary vector Agrobacterium strains to co-transform a plant. In this manner, heterologous nucleotide sequences of interest residing on different binary vectors can be independently introduced into the plant in a single transformation event and incorporated in the plant's nuclear DNA in an unlinked manner. The invention also provides for regenerated, fertile transgenic plants, transgenic seeds produced therefrom, and T1 and subsequent generations.

摘要翻译： 提供了植物有效共同转化的组合物和方法。 除了包含vir功能的辅助质粒之外，新型组合物是经过工程改造以包含至少两种二元载体质粒的农杆菌菌株。 每个二元载体包含在感兴趣的异源核苷酸序列侧翼的其自身的T-DNA边界。 本发明的方法包括使用这些新型多元二元载体农杆菌菌株共同转化植物。 以这种方式，驻留在不同二元载体上的感兴趣的异源核苷酸序列可以在单个转化事件中独立地引入植物中，并以非连接的方式并入植物的核DNA中。 本发明还提供了再生的，可育的转基因植物，由其产生的转基因种子，以及T1和随后的几代。

公开(公告)号：US09951346B2

公开(公告)日：2018-04-24

申请号：US14236597

申请日：2012-07-18

申请人： Andrew Mark Cigan ,  Saverio Carl Falco ,  Huirong Gao ,  Michael W. Lassner ,  Zhongsen Li ,  Zhan-Bin Liu ,  Leszek A. Lyznik ,  David J. Peterson ,  Christopher Jay Scelonge

发明人： Andrew Mark Cigan ,  Saverio Carl Falco ,  Huirong Gao ,  Michael W. Lassner ,  Zhongsen Li ,  Zhan-Bin Liu ,  Leszek A. Lyznik ,  David J. Peterson ,  Christopher Jay Scelonge

IPC分类号： C12N15/82

CPC分类号： C12N15/8213

摘要： Compositions and methods are provided for the targeted integration of a polynucleotide sequence of interest into the genome of a plant or plant cell. The methods and compositions employ recognition sites for endonucleases and endonucleases in combination with site-specific recombination sites/recombinases to provide an effective system for establishing target sites within the genome of a plant, plant cell or seed. Once such target sites are established, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest.