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公开(公告)号：US07994391B2
公开(公告)日：2011-08-09
申请号：US12018292
申请日：2008-01-23
申请人： Rebecca E. Cahoon , William James Gordon-Kamm , Yumin Tao , Keith S. Lowe , Christopher Jay Scelonge
发明人： Rebecca E. Cahoon , William James Gordon-Kamm , Yumin Tao , Keith S. Lowe , Christopher Jay Scelonge
CPC分类号： C12N15/8241 , C07K14/415 , C12N15/62 , C12N15/8201 , C12N15/8207 , C12N15/8261 , Y02A40/146
摘要： This invention relates to isolated polynucleotides encoding WUS polypeptides. The invention further provides isolated WUS polypeptides. The invention also provides methods of using the polynucleotides to modulate the level of WUS, improve transformation efficiency, to stimulate plant cell growth, including stem cells, to stimulate organogenesis, to stimulate somatic embryogenesis, to induce apomixis, and to provide a positive selection for cells comprising the polynucleotide. The invention also relates to cells, plants and seeds comprising the polynucleotides of the invention or produced by the methods of the invention.
摘要翻译： 本发明涉及编码WUS多肽的分离的多核苷酸。 本发明还提供了分离的WUS多肽。 本发明还提供了使用多核苷酸来调节WUS水平，提高转化效率，刺激植物细胞生长（包括干细胞）刺激器官发生，刺激体细胞胚发生，诱导无融合生殖以及提供阳性选择的方法 包含多核苷酸的细胞。 本发明还涉及包含本发明的多核苷酸或通过本发明的方法产生的细胞，植物和种子。
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公开(公告)号：US08383891B2
公开(公告)日：2013-02-26
申请号：US13168133
申请日：2011-06-24
申请人： Rebecca E. Cahoon , William James Gordon-Kamm , Keith S. Lowe , Christopher Jay Scelonge , Yumin Tao
发明人： Rebecca E. Cahoon , William James Gordon-Kamm , Keith S. Lowe , Christopher Jay Scelonge , Yumin Tao
CPC分类号： C12N15/8241 , C07K14/415 , C12N15/62 , C12N15/8201 , C12N15/8207 , C12N15/8261 , Y02A40/146
摘要： This invention relates to isolated polynucleotides encoding WUS polypeptides. The invention further provides isolated WUS polypeptides. The invention also provides methods of using the polynucleotides to modulate the level of WUS, improve transformation efficiency, to stimulate plant cell growth, including stem cells, to stimulate organogenesis, to stimulate somatic embryogenesis, to induce apomixis, and to provide a positive selection for cells comprising the polynucleotide. The invention also relates to cells, plants and seeds comprising the polynucleotides of the invention or produced by the methods of the invention.
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公开(公告)号：US07348468B1
公开(公告)日：2008-03-25
申请号：US09807946
申请日：2000-09-28
申请人： Rebecca E. Cahoon , Keith Lowe , William James Gordon-Kamm , Yumin Tao , Christopher Jay Scelonge
发明人： Rebecca E. Cahoon , Keith Lowe , William James Gordon-Kamm , Yumin Tao , Christopher Jay Scelonge
CPC分类号： C12N15/8241 , C07K14/415 , C12N15/62 , C12N15/8201 , C12N15/8207 , C12N15/8261 , Y02A40/146
摘要： This invention relates to an isolated nucleic acid fragment encoding a WUS protein. The invention also relates to the construction of a chimeric gene encoding all or a portion of the WUS protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the WUS protein in a transformed host cell.
摘要翻译： 本发明涉及编码WUS蛋白的分离的核酸片段。 本发明还涉及在正义或反义方向上构建编码WUS蛋白的全部或一部分的嵌合基因，其中嵌合基因的表达导致转化宿主细胞中WUS蛋白水平的改变。
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公开(公告)号：US20110258741A1
公开(公告)日：2011-10-20
申请号：US13168133
申请日：2011-06-24
申请人： Rebecca E. Cahoon , Keith S. Lowe , William J. Gordon-Kamm , Yumin Tao , Christopher J. Scelonge
发明人： Rebecca E. Cahoon , Keith S. Lowe , William J. Gordon-Kamm , Yumin Tao , Christopher J. Scelonge
CPC分类号： C12N15/8241 , C07K14/415 , C12N15/62 , C12N15/8201 , C12N15/8207 , C12N15/8261 , Y02A40/146
摘要： This invention relates to isolated polynucleotides encoding WUS polypeptides. The invention further provides isolated WUS polypeptides. The invention also provides methods of using the polynucleotides to modulate the level of WUS, improve transformation efficiency, to stimulate plant cell growth, including stem cells, to stimulate organogenesis, to stimulate somatic embryogenesis, to induce apomixis, and to provide a positive selection for cells comprising the polynucleotide. The invention also relates to cells, plants and seeds comprising the polynucleotides of the invention or produced by the methods of the invention.
摘要翻译： 本发明涉及编码WUS多肽的分离的多核苷酸。 本发明还提供了分离的WUS多肽。 本发明还提供了使用多核苷酸来调节WUS水平，提高转化效率，刺激植物细胞生长（包括干细胞）刺激器官发生，刺激体细胞胚发生，诱导无融合生殖以及提供阳性选择的方法 包含多核苷酸的细胞。 本发明还涉及包含本发明的多核苷酸或通过本发明的方法产生的细胞，植物和种子。
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5.发明授权Isolated LEC1 transcriptional regulator encoding nucleic acids and methods of use thereof 有权编码核酸的分离的LEC1转录调节子及其使用方法公开(公告)号：US07166765B2
公开(公告)日：2007-01-23
申请号：US10744620
申请日：2003-12-22
申请人： Keith S. Lowe , William J. Gordon-Kamm , Theodore M. Klein , Sonriza Rasco-Gaunt , Rebecca E. Cahoon , Xifan Sun , George J. Hoerster , Carolyn Gregory , Ramgopal Nadimpalli , Yumin Tao
发明人： Keith S. Lowe , William J. Gordon-Kamm , Theodore M. Klein , Sonriza Rasco-Gaunt , Rebecca E. Cahoon , Xifan Sun , George J. Hoerster , Carolyn Gregory , Ramgopal Nadimpalli , Yumin Tao
CPC分类号： C07K14/415 , C12N15/8209 , C12N15/8261 , C12N15/8287 , Y02A40/146
摘要： The invention provides isolated nucleic acids and their encoded proteins which act as transcriptional activators and methods of use thereof. The invention further provides expression cassettes, transformed host cells, transgenic plants and plant parts.
摘要翻译： 本发明提供分离的核酸及其编码的作为转录激活子的蛋白质及其使用方法。 本发明进一步提供表达盒，转化的宿主细胞，转基因植物和植物部分。
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公开(公告)号：US20100100981A1
公开(公告)日：2010-04-22
申请号：US12018292
申请日：2008-01-23
申请人： Rebecca E. Cahoon , Keith S. Lowe , William J. Gordon-Kamm , Yumin Tao , Christopher J. Scelonge
发明人： Rebecca E. Cahoon , Keith S. Lowe , William J. Gordon-Kamm , Yumin Tao , Christopher J. Scelonge
CPC分类号： C12N15/8241 , C07K14/415 , C12N15/62 , C12N15/8201 , C12N15/8207 , C12N15/8261 , Y02A40/146
摘要： This invention relates to isolated polynucleotides encoding WUS polypeptides. The invention further provides isolated WUS polypeptides. The invention also provides methods of using the polynucleotides to modulate the level of WUS, improve transformation efficiency, to stimulate plant cell growth, including stem cells, to stimulate organogenesis, to stimulate somatic embryogenesis, to induce apomixis, and to provide a positive selection for cells comprising the polynucleotide. The invention also relates to cells, plants and seeds comprising the polynucleotides of the invention or produced by the methods of the invention.
摘要翻译： 本发明涉及编码WUS多肽的分离的多核苷酸。 本发明还提供了分离的WUS多肽。 本发明还提供了使用多核苷酸来调节WUS水平，提高转化效率，刺激植物细胞生长（包括干细胞）刺激器官发生，刺激体细胞胚发生，诱导无融合生殖以及提供阳性选择的方法 包含多核苷酸的细胞。 本发明还涉及包含本发明的多核苷酸或通过本发明的方法产生的细胞，植物和种子。
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公开(公告)号：US07256322B2
公开(公告)日：2007-08-14
申请号：US10744572
申请日：2003-12-23
申请人： Keith S. Lowe , Rebecca E. Cahoon , Christopher J. Scelonge , Yumin Tao , William J. Gordon-Kamm , Wesley B. Bruce , Lisa J. Newman
发明人： Keith S. Lowe , Rebecca E. Cahoon , Christopher J. Scelonge , Yumin Tao , William J. Gordon-Kamm , Wesley B. Bruce , Lisa J. Newman
CPC分类号： C12N15/8207 , C07K14/415 , C12N15/8201 , C12N15/821 , C12N15/8241 , C12N15/8261 , Y02A40/146
摘要： This invention relates to isolated polynucleotides encoding WUS polypeptides. The invention further provides isolated WUS polypeptides. The invention also provides methods of using the polynucleotides to modulate the level of WUS, improve transformation efficiency, to stimulate plant cell growth, including stem cells, to stimulate organogenesis, to stimulate somatic embryogenesis, to induce apomixis, and to provide a positive selection for cells comprising the polynucleotide. The invention also relates to cells, plants and seeds comprising the polynucleotides of the invention or produced by the methods of the invention.
摘要翻译： 本发明涉及编码WUS多肽的分离的多核苷酸。 本发明还提供了分离的WUS多肽。 本发明还提供了使用多核苷酸来调节WUS水平，提高转化效率，刺激植物细胞生长（包括干细胞）刺激器官发生，刺激体细胞胚发生，诱导无融合生殖以及提供阳性选择的方法 包含多核苷酸的细胞。 本发明还涉及包含本发明的多核苷酸或通过本发明的方法产生的细胞，植物和种子。
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公开(公告)号：US06800791B1
公开(公告)日：2004-10-05
申请号：US09570319
申请日：2000-05-12
申请人： Matthew A. Bailey , Stanton B. Gelvin , Saikat Bhattacharjee , William J. Gordon-Kamm , Keith S. Lowe , Yumin Tao
发明人： Matthew A. Bailey , Stanton B. Gelvin , Saikat Bhattacharjee , William J. Gordon-Kamm , Keith S. Lowe , Yumin Tao
IPC分类号： A01H500
CPC分类号： C12N15/62 , C07H21/04 , C07K2319/09 , C07K2319/10 , C07K2319/61 , C07K2319/80 , C12N15/8205
摘要： The invention relates to the use of a modified Agrobacterium to deliver proteins directly to plant cells. Proteins of interest are delivered to the plant host in the form of a fusion protein with the Agrobacterium virulence protein VirF. Nucleotide sequences encoding such fusion proteins of VirF and a protein of interest are provided. Also provided are bacteria modified to comprise such fusion proteins of VirF and a protein of interest. Methods of introducing such fusion proteins into a plant host are provided. The invention finds use in facilitating plant transformation and particularly in the bio-engineering of desirable traits into crop plants.
摘要翻译： 本发明涉及使用改良的农杆菌将蛋白质直接递送至植物细胞。 感兴趣的蛋白质以与农杆菌毒力蛋白VirF融合蛋白的形式递送至植物宿主。 提供编码VirF和感兴趣蛋白质的融合蛋白的核苷酸序列。 还提供了细菌修饰以包含VirF和感兴趣的蛋白质的这种融合蛋白。 提供将这种融合蛋白引入植物宿主的方法。本发明用于促进植物转化，特别是在对作物植物期望的性状的生物工程中。
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9.发明申请Transcriptional Regulatory Nucleic Acids, Polypeptides and Methods of Use Thereof 审中-公开转录调控核酸，多肽及其使用方法公开(公告)号：US20080109925A1
公开(公告)日：2008-05-08
申请号：US11779552
申请日：2007-07-18
申请人： Yumin Tao , William J. Gordon-Kamm , Bo Shen , Keith S. Lowe , Olga N. Danilevskaya , Pramod B. Mahajan , Jan Antoni Rafalski , Hajime Sakai , Theodore M. Klein
发明人： Yumin Tao , William J. Gordon-Kamm , Bo Shen , Keith S. Lowe , Olga N. Danilevskaya , Pramod B. Mahajan , Jan Antoni Rafalski , Hajime Sakai , Theodore M. Klein
CPC分类号： C07K16/18 , C07K14/415 , C07K14/4702 , C07K2319/80 , C12N9/22 , C12N15/8201 , C12N15/8209 , C12N15/8247 , C12N15/8261 , C12N15/8287 , Y02A40/146
摘要： The invention provides isolated nucleic acids and their encoded proteins that act as cell transcription inhibitors and methods of use thereof. The invention further provides expression cassettes, transformed host cells, transgenic plants and plant parts, and antibody compositions.
摘要翻译： 本发明提供了作为细胞转录抑制剂的分离的核酸及其编码的蛋白质及其使用方法。 本发明进一步提供表达盒，转化的宿主细胞，转基因植物和植物部分以及抗体组合物。
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公开(公告)号：US20100285591A1
公开(公告)日：2010-11-11
申请号：US12844220
申请日：2010-07-27
申请人： Keith S. Lowe , Yumin Tao , William J. Gordon-Kamm , Carolyn A. Gregory , John A. McElver , George J. Hoerster , Sheila E. Maddock
发明人： Keith S. Lowe , Yumin Tao , William J. Gordon-Kamm , Carolyn A. Gregory , John A. McElver , George J. Hoerster , Sheila E. Maddock
CPC分类号： C12N15/8209 , C07K14/415 , C12N15/8201 , C12N15/8206 , C12N15/8207 , C12N15/8261 , Y02A40/146
摘要： The invention provides isolated polynucleotides, specifically Cyclin D polynucleotides, and their encoded proteins that are involved in cell cycle regulation. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions. The present invention provides methods and compositions relating to altering cell cycle protein content and/or composition of plants for the purpose of increasing transformation efficiency.
摘要翻译： 本发明提供分离的多核苷酸，特别是细胞周期蛋白D多核苷酸及其编码的参与细胞周期调节的蛋白质。 本发明还提供了重组表达盒，宿主细胞，转基因植物和抗体组合物。 本发明提供了改变植物的细胞周期蛋白质含量和/或组成以提高转化效率的方法和组合物。
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