公开(公告)号：US11965153B2

公开(公告)日：2024-04-23

申请号：US17517422

申请日：2021-11-02

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Matthew Angelini ,  Ashley Witmer ,  Anthony Debiase

IPC: C12M1/34 ,  C12M1/00

CPC classification number: C12M41/46 ,  C12M21/14 ,  C12M29/04 ,  C12M29/10 ,  C12M41/14 ,  C12M41/26 ,  C12M41/30 ,  C12M41/32 ,  C12M41/38 ,  C12M43/00

Abstract: A method of controlling a bioreactor system includes providing a cell culture in a bioreactor, wherein conditions in the bioreactor enable the cell culture to produce a protein of interest (POI), measuring process parameters (PPs) of the culture within the bioreactor by RAMAN, wherein the process parameters are selected from the group consisting of nutrient concentration, viable cell concentration, and protein attributes, measuring a predetermined weight of the bioreactor with the cell culture, removing cell-free spent media from the cell culture using a first output conduit at a first specified rate, removing cells from the cell culture using a second output conduit at a second specified rate, and introducing one or both of fresh media or nutrients into the cell culture using an input conduit at a third specified rate.

公开(公告)号：US11193103B2

公开(公告)日：2021-12-07

申请号：US16160465

申请日：2018-10-15

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Matthew Angelini ,  Ashley Witmer ,  Anthony Debiase

IPC: C12M1/00 ,  C12M1/34

Abstract: A method of controlling a bioreactor system includes providing a cell culture in a bioreactor, wherein conditions in the bioreactor enable the cell culture to produce a protein of interest (POI), measuring process parameters (PPs) of the culture within the bioreactor by RAMAN, wherein the process parameters are selected from the group consisting of nutrient concentration, viable cell concentration, and protein attributes, measuring a predetermined weight of the bioreactor with the cell culture, removing cell-free spent media from the cell culture using a first output conduit at a first specified rate, removing cells from the cell culture using a second output conduit at a second specified rate, and introducing one or both of fresh media or nutrients into the cell culture using an input conduit at a third specified rate.