公开(公告)号：US20240263110A1

公开(公告)日：2024-08-08

申请号：US18410960

申请日：2024-01-11

Applicant: POET Research, Inc.

Inventor： Jason Alan Bootsma

IPC: C12F3/10 ,  A23K10/38 ,  A23K20/163 ,  A23K50/10 ,  C10L1/02 ,  C11B1/00 ,  C12M1/00 ,  C12M1/34

CPC classification number: C12F3/10 ,  A23K10/38 ,  A23K20/163 ,  A23K50/10 ,  C10L1/026 ,  C11B1/00 ,  C12M41/26 ,  C12M43/00 ,  C12M47/10 ,  C12M47/14 ,  Y02P60/87

Abstract: Systems and methods for fractionating whole stillage from an ethanol production facility are provided. Whole stillage undergoes a separation of its liquid portion (thin stillage) from the solid portion (fiber cake). In some embodiments, the solids and liquids in whole stillage may be separated utilizing a screening centrifuge. The fiber cake may be dried to generate a high fiber animal feed. The thin stillage may be provided to a three-phase separator for separation into an oil emulsion, an aqueous clarified stillage, and a protein paste. The protein paste may be dried to generate a high protein animal feed with greater than about 45% protein content. The clarified thin stillage is condensed to yield a syrup with greater than around 60% solids. The oil emulsion is subjected to a pH adjustment to liberate the oil from the emulsion, which is then separated.

公开(公告)号：US11965153B2

公开(公告)日：2024-04-23

申请号：US17517422

申请日：2021-11-02

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Matthew Angelini ,  Ashley Witmer ,  Anthony Debiase

IPC: C12M1/34 ,  C12M1/00

CPC classification number: C12M41/46 ,  C12M21/14 ,  C12M29/04 ,  C12M29/10 ,  C12M41/14 ,  C12M41/26 ,  C12M41/30 ,  C12M41/32 ,  C12M41/38 ,  C12M43/00

Abstract: A method of controlling a bioreactor system includes providing a cell culture in a bioreactor, wherein conditions in the bioreactor enable the cell culture to produce a protein of interest (POI), measuring process parameters (PPs) of the culture within the bioreactor by RAMAN, wherein the process parameters are selected from the group consisting of nutrient concentration, viable cell concentration, and protein attributes, measuring a predetermined weight of the bioreactor with the cell culture, removing cell-free spent media from the cell culture using a first output conduit at a first specified rate, removing cells from the cell culture using a second output conduit at a second specified rate, and introducing one or both of fresh media or nutrients into the cell culture using an input conduit at a third specified rate.

公开(公告)号：US20230340397A9

公开(公告)日：2023-10-26

申请号：US16579889

申请日：2019-09-24

Applicant: Christopher B. REID

Inventor： Christopher B. REID ,  Melissa BRAGA ,  Lilian N. SANTAMARIA ,  Ashley N. WICKREMA ,  Marinne D. WICKREMA ,  Anthony HAO DINH ,  Yaman EKSIOGLU ,  Mat HOANG HO

IPC: C12M1/00 ,  C12N15/10 ,  C12N15/11 ,  C12M3/00 ,  C12M1/42

CPC classification number: C12M43/00 ,  C12M21/00 ,  C12N15/102 ,  C12N15/111 ,  C12M23/42 ,  C12M35/02

Abstract: The claimed invention is directed towards a novel combination cell electroporation/cell culturing apparatus which can be termed a cell culture dish suitable for in vitro electroporation, and towards a device suitable for in vivo electroporation—both useful in methods suitable for the generation of developmentally-activated, pluripotent, pluripotent-like, multipotent, and/or self-renewing cells which are capable of beginning to differentiate in culture into a variety of cell types and capable of further differentiation in vivo. The claimed invention is also directed towards the generation of desirable, differentiating somatic cell populations transplantable to animals or patients, genetic modification of endogenous and exogenous cells, and the treatment of patients suffering from diseases that may be ameliorated by these methods. This invention also provides methods for preventing, treating, or retarding disease, for example, immunodeficiency virus (e.g. HIV-1, HIV-2, SIV, FIV, etc.) infection.

公开(公告)号：US20230181796A1

公开(公告)日：2023-06-15

申请号：US17925187

申请日：2021-05-14

Applicant: North Carolina State University

Inventor： Donald O. Freytes ,  Camilo A. Mora-Navarro ,  Andreea Badileanu

IPC: A61L27/36 ,  C12M3/00 ,  C12M1/00 ,  C12M1/26 ,  C12M1/06 ,  C12M1/34 ,  A61L27/22 ,  C12M1/36

CPC classification number: A61L27/3683 ,  C12M21/08 ,  A61L27/3633 ,  C12M43/00 ,  C12M29/00 ,  C12M33/14 ,  C12M27/02 ,  C12M41/12 ,  C12M41/44 ,  A61L27/225 ,  A61L27/227 ,  C12M41/48 ,  C12M41/32 ,  A61L2430/40 ,  A61L2400/06

Abstract: Provided herein are systems, devices and methods to automate and optimize the decellularization process of representative tissues, such as soft tissues, for extracellular matrix (ECM)-based scaffold and biomaterial production. The automated decellularization processes and devices significantly reduce the exposure time to reagents, minimize lot-to-lot variability, and largely preserve the native composition of the ECM from the decellularized tissue or species.

公开(公告)号：US10005997B2

公开(公告)日：2018-06-26

申请号：US15113378

申请日：2016-06-01

Applicant: KOREA DISTRICT HEATING CORP.

Inventor： Won Seok Chang ,  Deog Yong Ahn ,  Sang Ho Shin ,  Ji Hye Yu ,  Young Jae Lee ,  Won Kwon Lee ,  Kwang Keun Choi ,  Sung Min Kim

IPC: C12M1/04 ,  C12M1/26 ,  C12M1/00

CPC classification number: C12M23/40 ,  C12M21/02 ,  C12M23/58 ,  C12M29/06 ,  C12M33/00 ,  C12M43/00

Abstract: The present invention relates to a port installed in a closed-ended photochemical reactor, and more particularly, to a multi-functional port for microalgae cultivation and harvesting that supplies gas for cultivation for culturing microalgae by connecting to the closed-ended photochemical reactor; supplies gas for prevention of precipitation for lifting microalgae during cultivation; and assembles by taking samples of microalgae or selectively combining a plurality of valves which can harvest microalgae, whose cultivation is completed.

公开(公告)号：US09914942B2

公开(公告)日：2018-03-13

申请号：US14684846

申请日：2015-04-13

Applicant: Ronald E. Arnoldsen, Jr. ,  Debra A. Arnoldsen

Inventor： Ronald E. Arnoldsen, Jr. ,  Debra A. Arnoldsen

IPC: C02F3/28 ,  C12P5/02 ,  C12M1/107 ,  C12M1/12 ,  C02F11/04 ,  C12M1/00 ,  C12M1/26 ,  C12M1/02 ,  C02F103/20

CPC classification number: C12P5/023 ,  C02F3/284 ,  C02F11/04 ,  C02F2103/20 ,  C12M21/04 ,  C12M23/04 ,  C12M23/06 ,  C12M23/34 ,  C12M23/36 ,  C12M33/00 ,  C12M41/22 ,  C12M43/00 ,  C12M47/18 ,  Y02E50/343 ,  Y02W10/23 ,  Y02W10/37

Abstract: An anaerobic digestion device includes a digester body with an opening through which organic waste is received and a digester drum removably inserted into the digester body for receiving the organic waste. The digester drum is movable relative to the digester body to advance a slurry of the organic waste along a length of the digester body. A set of ports spaced along the digester body and arranged to vent biogas from the organic waste contained in the digester drum. A storage vessel is configured to receive and store biogas received from the digester body via the ports, and a heating system configured to heat the digester body. The heating system is fuelled by the biogas vented from the digester body.

公开(公告)号：US20180056307A1

公开(公告)日：2018-03-01

申请号：US15556015

申请日：2016-02-15

Applicant: Valmet AB

Inventor： Patrik Pettersson ,  Johan Lindberg

IPC: B04C5/04 ,  C12M1/00

CPC classification number: B04C5/04 ,  B04C5/081 ,  C12M43/00

Abstract: A cyclone separator (10) comprises a pressure chamber (20), an inlet (30) for an incoming flow of a mixture of gas and particles, a gas outlet (50) for outgoing gas arranged through a top wall (26) of the pressure chamber and a particle outlet (40) for outgoing particles arranged in a lower part (22) of the pressure chamber. The pressure chamber has a main rotation symmetric shape. The inlet is arranged through a side wall (28) of an upper part (24) of the pressure chamber for directing the incoming flow with a main velocity component in a tangential direction. The inlet comprises an inlet tube (36) protruding through the side wall of the upper part into the pressure chamber, whereby an inner end (38) of the inlet tube is provided at a position interior of the pressure chamber. A method for operating a cyclone separator is also disclosed.

公开(公告)号：US09797597B2

公开(公告)日：2017-10-24

申请号：US14483798

申请日：2014-09-11

Applicant: Board of Regents, The University of Texas System

Inventor： Halil Berberoglu ,  Rhykka L. Connelly ,  Joey Anthony ,  Cody Bond

IPC: C12M1/00 ,  F23G5/027 ,  C12M1/107 ,  C10G1/00 ,  C10G1/06 ,  C12P5/02

CPC classification number: F23G5/027 ,  C10G1/00 ,  C10G1/065 ,  C10G2300/1003 ,  C10G2300/1007 ,  C10G2300/1011 ,  C10G2300/1014 ,  C10G2300/1018 ,  C12M21/02 ,  C12M21/04 ,  C12M21/12 ,  C12M43/00 ,  C12M43/08 ,  C12P5/023 ,  F23G2900/50803 ,  Y02E20/12 ,  Y02E50/14 ,  Y02E50/343 ,  Y02P20/129 ,  Y02P20/134 ,  Y02P20/136 ,  Y02P30/20 ,  Y02W10/37

Abstract: Systems and processes of providing novel thermal energy sources for hydrothermal liquefaction (HTL) reactors are described herein. According to various implementations, the systems and processes use concentrated solar thermal energy from a focused high-energy beam to provide sufficient energy for driving the HTL biomass-to-biocrude process. In addition, other implementations convert biowaste, such as municipal biosolids and grease and food waste, to biocrude using anaerobic digesters, and a portion of the biogas generated by the digesters is used to produce the thermal and/or electrical energy used in the HTL reactor for the biomass-to-biocrude process. Furthermore, alternative implementations may include a hybrid system that uses biogas and solar radiation to provide sufficient thermal energy for the HTL reactor.

公开(公告)号：US09745601B2

公开(公告)日：2017-08-29

申请号：US14377223

申请日：2013-02-15

Applicant: The Trustees of Columbia University in the City of New York

Inventor： Scott Banta ,  Alan West ,  Timothy Kernan

IPC: C12P1/04 ,  C12M1/00 ,  C12P7/16 ,  C12N1/20 ,  C12N9/04 ,  C12N9/88 ,  C12P3/00 ,  C12P5/02 ,  C12P7/04

CPC classification number: C12P1/04 ,  C12M21/12 ,  C12M43/00 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/88 ,  C12P3/00 ,  C12P5/02 ,  C12P7/04 ,  C12P7/16 ,  Y02E50/10

Abstract: Methods and systems for producing a biofuel using genetically modified sulfur-oxidizing and iron-reducing bacteria (SOIRB) are disclosed. In some embodiments, the methods include the following: providing a SOIRB that have been genetically modified to include a particular metabolic pathway to enable them to generate a biofuel; feeding a first source of ferric iron to the SOIRB; feeding sulfur, water, and carbon dioxide to the SOIRB; producing at least the first particular biofuel, a first source of ferrous iron, sulfate, excess ferric iron, and an SOIRB biomass; electrochemically reducing the excess ferric iron to a second source of ferrous iron; providing an iron-oxidizing bacteria that have been genetically modified to include a particular metabolic pathway to enable them to generate a second biofuel; producing at least the second biofuel, a second source of ferric iron, and an IOB biomass; and feeding the second source of ferric iron to the SOIRB.

公开(公告)号：US20170226454A1

公开(公告)日：2017-08-10

申请号：US15428800

申请日：2017-02-09

Applicant: UTI Limited Partnership

Inventor： Marc STROUS ,  Christine SHARP ,  Hector de la HOZ SIEGLER ,  Gregory WELCH

IPC: C12M1/00 ,  C12M1/107 ,  C12M1/34

CPC classification number: C12M21/02 ,  C12M21/04 ,  C12M23/22 ,  C12M23/36 ,  C12M23/58 ,  C12M41/34 ,  C12M43/00

Abstract: A system and a method for producing biomass from a mixed community of algal species. The method comprises the steps of culturing the mixed community of at least two algal species as biofilms on transparent surfaces having structural features and an optical filter, providing a continuous supply of a culture medium comprising at least 0.5 mol/L aqueous (bi)carbonate and having a pH greater than 9. The method disclosed herein facilitates online monitoring of mixed community productivity by the quantification of oxygen production.