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1.发明申请Methods and apparatus for electrophoresis of prior-cast, hydratable separation media 审中-公开预浇铸水合分离介质的电泳方法和装置公开(公告)号：US20050284764A1
公开(公告)日：2005-12-29
申请号：US11179823
申请日：2005-07-11
IPC分类号： B01D57/02 , B03C5/00 , C07K1/26 , C08F2/58 , G01N27/447 , G01N33/559 , G01N33/561 , B01D59/42
CPC分类号： G01N27/44795 , C07K1/26 , G01N27/44704 , G01N33/559 , G01N33/561
摘要： Methods and apparatus are presented that facilitate electrophoresis of prior-cast, hydratable separation media, usefully immobilized pH gradient (IPG) strips. The method exploits the swelling of prior-cast, hydratable separation media upon rehydration to help lodge the media in an enclosing member that permits spaced electrical communication with the enclosed separation media. The electrical communication permits a voltage gradient to be established in the enclosed separation medium sufficient to effect separation of analytes therein. Cassettes, buffer cores, electrophoresis systems and kits are presented for effecting the methods of the invention.
摘要翻译： 提出了方便和装置，其促进了预先浇铸的可水合分离介质的电泳，有用的固定化pH梯度（IPG）条带。 该方法利用了预先浇铸的可水合分离介质在再水合时的溶胀，以帮助将介质置于封闭的构件中，该封闭构件允许与封闭的分离介质间隔开的电连通。 电气通信允许在封闭的分离介质中建立足以实现其中分析物分离的电压梯度。 提出了盒，缓冲核心，电泳系统和试剂盒用于实现本发明的方法。
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2.发明授权公开(公告)号：US08034223B2
公开(公告)日：2011-10-11
申请号：US12485379
申请日：2009-06-16
IPC分类号： G01N27/447 , G01N27/453
CPC分类号： C07K1/26 , G01N27/44704 , G01N27/44747 , G01N27/44795 , G01N33/559 , G01N33/561
摘要： Methods and apparatus are presented that facilitate electrophoresis of prior-cast, hydratable separation media, usefully immobilized pH gradient (IPG) strips. The method exploits the swelling of prior-cast, hydratable separation media upon rehydration to help lodge the media in an enclosing member that permits spaced electrical communication with the enclosed separation media. The electrical communication permits a voltage gradient to be established in the enclosed separation medium sufficient to effect separation of analytes therein. Cassettes, buffer cores, electrophoresis systems and kits are presented for effecting the methods of the invention.
摘要翻译： 提出了方便和装置，其促进了预先浇铸的可水合分离介质的电泳，有用的固定化pH梯度（IPG）条带。 该方法利用了预先浇铸的可水合分离介质在再水合时的溶胀，以帮助将介质置于封闭的构件中，该封闭构件允许与封闭的分离介质间隔开的电连通。 电气通信允许在封闭的分离介质中建立足以实现其中分析物分离的电压梯度。 提出了盒，缓冲核心，电泳系统和试剂盒用于实现本发明的方法。
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公开(公告)号：US5578180A
公开(公告)日：1996-11-26
申请号：US221939
申请日：1994-03-31
IPC分类号： G01N27/447 , G01N27/26
CPC分类号： G01N27/44747
摘要： A gel and buffer system for gel electrophoresis wherein separation occurs at neutral pH and proteins remain completely reduced.
摘要翻译： 用于凝胶电泳的凝胶和缓冲系统，其中在中性pH下进行分离，蛋白质保持完全降低。
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4.发明申请公开(公告)号：US20090314641A1
公开(公告)日：2009-12-24
申请号：US12485379
申请日：2009-06-16
IPC分类号： G01N27/447
CPC分类号： C07K1/26 , G01N27/44704 , G01N27/44747 , G01N27/44795 , G01N33/559 , G01N33/561
摘要： Methods and apparatus are presented that facilitate electrophoresis of prior-cast, hydratable separation media, usefully immobilized pH gradient (IPG) strips. The method exploits the swelling of prior-cast, hydratable separation media upon rehydration to help lodge the media in an enclosing member that permits spaced electrical communication with the enclosed separation media. The electrical communication permits a voltage gradient to be established in the enclosed separation medium sufficient to effect separation of analytes therein. Cassettes, buffer cores, electrophoresis systems and kits are presented for effecting the methods of the invention.
摘要翻译： 提出了方便和装置，其促进了预先浇铸的可水合分离介质的电泳，有用的固定化pH梯度（IPG）条带。 该方法利用了预先浇铸的可水合分离介质在再水合时的溶胀，以帮助将介质置于封闭的构件中，该封闭构件允许与封闭的分离介质间隔开的电连通。 电气通信允许在封闭的分离介质中建立足以实现其中分析物分离的电压梯度。 提出了盒，缓冲核心，电泳系统和试剂盒用于实现本发明的方法。
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5.发明授权公开(公告)号：US07601251B2
公开(公告)日：2009-10-13
申请号：US10464258
申请日：2003-06-17
IPC分类号： G01N27/447 , G01N27/453
CPC分类号： C07K1/26 , G01N27/44704 , G01N27/44747 , G01N27/44795 , G01N33/559 , G01N33/561
摘要： Methods and apparatus are presented that facilitate electrophoresis of prior-cast, hydratable separation media, usefully immobilized pH gradient (IPG) strips. The method exploits the swelling of prior-cast, hydratable separation media upon rehydration to help lodge the media in an enclosing member that permits spaced electrical communication with the enclosed separation media. The electrical communication permits a voltage gradient to be established in the enclosed separation medium sufficient to effect separation of analytes therein. Cassettes, buffer cores, electrophoresis systems and kits are presented for effecting the methods of the invention.
摘要翻译： 提出了方便和装置，其促进了预先浇铸的可水合分离介质的电泳，有用的固定化pH梯度（IPG）条带。 该方法利用了预先浇铸的可水合分离介质在再水合时的溶胀，以帮助将介质置于封闭的构件中，该封闭构件允许与封闭的分离介质间隔开的电连通。 电气通信允许在封闭的分离介质中建立足以实现其中分析物分离的电压梯度。 提出了盒，缓冲核心，电泳系统和试剂盒用于实现本发明的方法。
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公开(公告)号：US5572802A
公开(公告)日：1996-11-12
申请号：US511183
申请日：1995-08-04
CPC分类号： F26B9/04
摘要： A method and apparatus for drying electrophoresis gels. The apparatus includes a base unit and gel drying frame assembly. The base unit serves as a both a support for the frame when placing the gel in the frame and as a receptacle for excess liquid used to prepare the gel for the drying process. The frame is used to securely hold the gel in place while preparing the gel for drying and also during the drying process itself.
摘要翻译： 一种用于干燥电泳凝胶的方法和装置。 该装置包括基座单元和凝胶干燥框架组件。 当将凝胶放置在框架中时，基座单元用作框架的支撑件，并且用作用于制备用于干燥过程的凝胶的过量液体的容器。 该框架用于在制备用于干燥的凝胶以及干燥过程本身期间将凝胶牢固地保持在适当位置。
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公开(公告)号：US5685967A
公开(公告)日：1997-11-11
申请号：US573620
申请日：1995-12-18
申请人： David J. Manis , Sheldon Engelhorn
发明人： David J. Manis , Sheldon Engelhorn
IPC分类号： G01N27/447 , B01D61/42
CPC分类号： G01N27/44756 , G01N27/453
摘要： An improved coated plastic mold for the polymerization of electrophoresis gels. The mold controls the effects of oxygen contamination upon gel polymerization resulting in a more uniform gel with improved properties.
摘要翻译： 一种用于电泳凝胶聚合的改进的涂覆塑料模具。 模具控制氧气污染对凝胶聚合的影响，从而形成具有改进性能的更均匀的凝胶。
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公开(公告)号：US06936150B2
公开(公告)日：2005-08-30
申请号：US10102188
申请日：2002-03-18
IPC分类号： B01D57/02 , B03C5/00 , C07K1/26 , C08F2/58 , G01N27/447 , G01N33/559 , G01N33/561 , G01N27/453
CPC分类号： G01N27/44795 , C07K1/26 , G01N27/44704 , G01N33/559 , G01N33/561
摘要： Methods and apparatus are presented that facilitate electrophoresis of prior-cast, hydratable separation media, usefully immobilized pH gradient (IPG) strips. The method exploits the swelling of prior-cast, hydratable separation media upon rehydration to help lodge the media in an enclosing member that permits spaced electrical communication with the enclosed separation media. The electrical communication permits a voltage gradient to be established in the enclosed separation medium sufficient to effect separation of analytes therein. Cassettes, buffer cores, electrophoresis systems and kits are presented for effecting the methods of the invention.
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公开(公告)号：US5753095A
公开(公告)日：1998-05-19
申请号：US716680
申请日：1996-09-16
IPC分类号： G01N27/447 , G01N27/26
CPC分类号： G01N27/44704
摘要： An improved plastic mold for the polymerization of electrophoresis gels. The improved electrophoresis gel mold comprises non-conductive plastic film. The film is supported by a rigid plastic frame. The frame maintains the integrity of the electrophoresis gel. The molds of the present invention are particularly useful in the manufacture of precast electrophoresis gels.
摘要翻译： 用于电泳凝胶聚合的改进的塑料模具。 改进的电泳凝胶模具包括非导电塑料膜。 胶片由刚性塑料框架支撑。 该框架保持电泳凝胶的完整性。 本发明的模具特别可用于制造预制电泳凝胶。
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公开(公告)号：US5440822A
公开(公告)日：1995-08-15
申请号：US93105
申请日：1993-07-16
CPC分类号： F26B9/04
摘要： A method and apparatus for drying electrophoresis gels. The apparatus includes a base unit and gel drying frame assembly. The base unit serves as a both a support for the frame when placing the gel in the frame and as a receptacle for excess liquid used to prepare the gel for the drying process. The frame is used to securely hold the gel in place while preparing the gel for drying and also during the drying process itself.
摘要翻译： 一种用于干燥电泳凝胶的方法和装置。 该装置包括基座单元和凝胶干燥框架组件。 当将凝胶放置在框架中时，基座单元用作框架的支撑件，并且用作用于制备用于干燥过程的凝胶的过量液体的容器。 该框架用于在制备用于干燥的凝胶以及干燥过程本身期间将凝胶牢固地保持在适当位置。
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