公开(公告)号：US08580935B2

公开(公告)日：2013-11-12

申请号：US12528670

申请日：2008-02-21

申请人： Reno T. Nguyen ,  Scott Anderson ,  Ian Chappell

发明人： Reno T. Nguyen ,  Scott Anderson ,  Ian Chappell

IPC分类号： C07K1/22 ,  B01D15/08

CPC分类号： C07K1/22 ,  B01D15/325 ,  B01J20/28004 ,  B01J20/28083 ,  B01J20/283 ,  B01J20/287 ,  C07K1/20 ,  C07K16/065

摘要： The present invention relates to a chromatographic method of separating biological material comprising, providing chromatographic media comprising inorganic oxide particles having an average diameter of about 2 microns or less and an average pore diameter of 300 Å or more; applying a solvent comprising said biological material to said media, wherein said biological material is reversibly bonded to said media; and eluting said biological material from said media with a solvent in less than about 2 minutes for biological material having a molecular weight of less than about 100,000 Daltons.

摘要翻译： 本发明涉及分离生物材料的层析方法，包括：提供包含平均直径约2微米或更小且平均孔径为300埃或更大的无机氧化物颗粒的色谱介质; 将包含所述生物材料的溶剂施加到所述介质上，其中所述生物材料可逆地结合到所述介质上; 并用少于大约2分钟的时间用溶剂从所述培养基中洗脱所述生物材料，用于分子量小于约100,000道尔顿的生物材料。

公开(公告)号：US20100167374A1

公开(公告)日：2010-07-01

申请号：US12528670

申请日：2008-02-21

申请人： Reno T. Nguyen ,  Scott Anderson ,  Ian Chappell

发明人： Reno T. Nguyen ,  Scott Anderson ,  Ian Chappell

IPC分类号： C07K1/16 ,  C07K14/80 ,  C07K14/765 ,  C07K14/61 ,  C07K14/805 ,  C07K16/00 ,  C07K14/77 ,  C07K14/76 ,  C07K14/62 ,  C12N9/00

CPC分类号： C07K1/22 ,  B01D15/325 ,  B01J20/28004 ,  B01J20/28083 ,  B01J20/283 ,  B01J20/287 ,  C07K1/20 ,  C07K16/065

摘要： The present invention relates to a chromatographic method of separating biological material comprising, providing chromatographic media comprising inorganic oxide particles having an average diameter of about 2 microns or less and an average pore diameter of 300 Å or more; applying a solvent comprising said biological material to said media, wherein said biological material is reversibly bonded to said media; and eluting said biological material from said media with a solvent in less than about 2 minutes for biological material having a molecular weight of less than about 100,000 Daltons.

摘要翻译： 本发明涉及分离生物材料的层析方法，包括：提供包含平均直径约2微米或更小且平均孔径为300埃或更大的无机氧化物颗粒的色谱介质; 将包含所述生物材料的溶剂施加到所述介质上，其中所述生物材料可逆地结合到所述介质上; 并用少于大约2分钟的时间用溶剂从所述培养基中洗脱所述生物材料，用于分子量小于约100,000道尔顿的生物材料。