公开(公告)号：US20240116029A1

公开(公告)日：2024-04-11

申请号：US18275356

申请日：2022-01-24

Applicant: BIOCON BIOLOGIES LIMIED

Inventor： Sathish ALAGURAJAN ,  Sandeep V. KAMATH ,  Shabanri QAIS

IPC: B01J20/34 ,  B01D15/20 ,  B01J20/283 ,  B01J20/287

CPC classification number: B01J20/3475 ,  B01D15/203 ,  B01J20/283 ,  B01J20/287 ,  B01J20/3433

Abstract: The present invention relates to a process for the regeneration of the spent silica resin used in chromatography without unpacking resin from chromatography column. The process comprises 3 steps, namely: contacting the chromatography resin with regeneration solution, neutralizing the regenerated chromatography resin with neutralization solution and washing the neutralized chromatography resin with wash solution.

公开(公告)号：US11740163B2

公开(公告)日：2023-08-29

申请号：US16160925

申请日：2018-10-15

Applicant: DIONEX CORPORATION

Inventor： Xiaodong Liu ,  Christopher A. Pohl

IPC: B01J20/286 ,  B01J20/287 ,  B01J20/289 ,  B01J20/32 ,  G01N1/40 ,  C07F7/21

CPC classification number: G01N1/405 ,  B01J20/286 ,  B01J20/287 ,  B01J20/289 ,  B01J20/3219 ,  B01J20/3246 ,  B01J20/3257 ,  C07F7/21 ,  B01J2220/54

Abstract: A method of separating analytes in a liquid sample includes flowing the liquid sample through a chromatography column configured for use in liquid chromatography. The chromatography column contains a composition that includes a solid support having an exterior surface, a ligand, and a linker. The ligand includes a polyhedral oligomeric silsesquioxane moiety. The linker is covalently bound to both the polyhedral oligomeric silsesquioxane moiety and the exterior surface of the solid support.

公开(公告)号：US20190211272A1

公开(公告)日：2019-07-11

申请号：US16358545

申请日：2019-03-19

Applicant: The University of Wyoming Research Corporation dba Western Research Institute

Inventor： John F. Schabron ,  Joseph F. Rovani, JR. ,  Jean-Pascal Planche

IPC: C10G25/00 ,  B01D15/26 ,  B01D15/00 ,  G01N30/46 ,  C10G53/08 ,  G01N33/28 ,  B01J20/281 ,  B01J20/286 ,  B01J20/287 ,  C10G25/12 ,  G01N30/14 ,  B01J20/284 ,  B01J20/283

CPC classification number: C10G25/003 ,  B01D15/00 ,  B01D15/265 ,  B01J20/281 ,  B01J20/283 ,  B01J20/284 ,  B01J20/286 ,  B01J20/287 ,  C10G25/12 ,  C10G53/08 ,  C10G2400/30 ,  G01N30/14 ,  G01N30/461 ,  G01N30/468 ,  G01N33/2823 ,  G01N33/2835 ,  G01N2030/027 ,  G01N2030/085 ,  G01N2030/143 ,  G01N2030/8854

Abstract: A method for determining asphaltene stability in a hydrocarbon-containing material having solvated asphaltenes therein is disclosed. In at least one embodiment, it involves the steps of: (a) precipitating an amount of the asphaltenes from a liquid sample of the hydrocarbon-containing material with an alkane mobile phase solvent in a column; (b) dissolving a first amount and a second amount of the precipitated asphaltenes by changing the alkane mobile phase solvent to a final mobile phase solvent having a solubility parameter that is higher than the alkane mobile phase solvent; (c) monitoring the concentration of eluted fractions from the column; (d) creating a solubility profile of the dissolved asphaltenes in the hydrocarbon-containing material; and (e) determining one or more asphaltene stability parameters of the hydrocarbon-containing material.

公开(公告)号：US20180001229A1

公开(公告)日：2018-01-04

申请号：US15536506

申请日：2015-12-16

Applicant: Waters Technologies Corporation

Inventor： Pamela C. Iraneta ,  Xin Zhang ,  Frank John Marszalkowski, Jr.

IPC: B01D15/38 ,  B01J20/287 ,  B01J20/288 ,  B01J20/32 ,  B01J20/28 ,  G01N30/00 ,  G01N1/40

CPC classification number: B01D15/3847 ,  B01J20/28052 ,  B01J20/287 ,  B01J20/288 ,  B01J20/3204 ,  B01J20/3217 ,  B01J20/3246 ,  B01J20/3285 ,  B01J20/3293 ,  G01N30/50 ,  G01N30/72 ,  G01N2001/4061 ,  G01N2030/009

Abstract: Disclosed is a device for a solid phase extraction comprising two or more of the sorbents to remove phospholipids and salts from a sample, to thereby eliminate matrix effects during mass spectrometry analysis. In particular, the sorbents includes at least one sorbent which is water-wettable and contains at least one hydrophobic component and at least one hydrophilic component and at least one of sorbent having a specific affinity for a matrix interference like phospholipids. Further disclosed is a method using the device of the present invention.

公开(公告)号：US09353317B2

公开(公告)日：2016-05-31

申请号：US13237568

申请日：2011-09-20

Applicant: John F. Schabron ,  Ryan B. Boysen ,  Eric W. Kalberer ,  Joseph F. Rovani, Jr.

Inventor： John F. Schabron ,  Ryan B. Boysen ,  Eric W. Kalberer ,  Joseph F. Rovani, Jr.

IPC: C10G53/08 ,  C10G25/00 ,  B01D15/26 ,  C10G25/12 ,  G01N30/46 ,  G01N33/28 ,  B01J20/281 ,  B01J20/283 ,  B01J20/284 ,  B01J20/286 ,  B01J20/287 ,  B01D15/00 ,  G01N30/08 ,  G01N30/88

CPC classification number: C10G53/08 ,  B01D15/00 ,  B01D15/08 ,  B01D15/265 ,  B01J20/103 ,  B01J20/281 ,  B01J20/283 ,  B01J20/284 ,  B01J20/286 ,  B01J20/287 ,  B01J20/288 ,  B01J2220/603 ,  C10G25/00 ,  C10G25/003 ,  C10G25/12 ,  C10G2300/206 ,  C10G2400/30 ,  G01N30/14 ,  G01N30/461 ,  G01N30/468 ,  G01N33/2823 ,  G01N33/2835 ,  G01N2030/027 ,  G01N2030/085 ,  G01N2030/143 ,  G01N2030/8854

Abstract: The inventive technology may involve, in particular embodiments, novel use of a non-porous, high surface energy stationary phase to adsorb, in reversible fashion, the most polar component of a resins fraction of an input hydrocarbon when a mobile phase is passed over the stationary phase. Such reversible adsorption prevents irreversibly adsorption of such components on active stationary phase(s) downflow of the non-porous, high surface energy stationary phase, thereby conserving stationary phase costs and increasing resolution of resins elutions, and accuracy of hydrocarbon component results. Aspects of the inventive technology may also involve a novel combination of a solubility based asphaltene component fractionating and analysis method and an adsorption chromatography method for separating and/or analyzing saturate, aromatics and resins components of an input hydrocarbon.

Abstract translation: 在具体实施方案中，本发明的技术可以涉及无孔高表面能固定相的新用途，以可逆的方式吸附输入烃的树脂级分的最极性组分，当流动相通过 固定相。 这种可逆吸附防止这些组分在无孔，高表面能固定相的活性固定相向下流动中的不可逆吸附，从而保持固定相成本和提高树脂洗脱液的分离度以及烃组分结果的准确性。 本发明技术的方面还可以涉及一种基于溶解度的沥青质组分分馏和分析方法的新型组合以及用于分离和/或分析输入烃的饱和物，芳族化合物和树脂组分的吸附层析方法。

公开(公告)号：US20160139014A1

公开(公告)日：2016-05-19

申请号：US15006441

申请日：2016-01-26

Applicant: DIONEX CORPORATION

Inventor： Xiaodong LIU ,  Christopher A. POHL

IPC: G01N1/40

CPC classification number: G01N1/405 ,  B01J20/286 ,  B01J20/287 ,  B01J20/289 ,  B01J20/3219 ,  B01J20/3246 ,  B01J20/3257 ,  B01J2220/54 ,  C07F7/21

Abstract: Ligand compositions and stationary phases comprising polyhedral oligomeric silsesquioxane moieties are incorporated in to chromatographic stationary phases, and these phases are incorporated into chromatography devices, such as columns. The compositions and devices are of use to separate molecular mixtures.

Abstract translation: 将配体组合物和包含多面体低聚倍半硅氧烷部分的固定相并入到色谱固定相中，并将这些相结合到诸如柱的色谱装置中。 组合物和装置用于分离分子混合物。

公开(公告)号：US09216403B2

公开(公告)日：2015-12-22

申请号：US13841484

申请日：2013-03-15

Applicant: DIONEX CORPORATION

Inventor： Xiaodong Liu ,  Liang Cao ,  Christopher A. Pohl

IPC: C07F7/08 ,  B01J20/22 ,  B01J20/286 ,  B01J20/287 ,  B01J20/32 ,  B01D15/20 ,  G01N30/02

CPC classification number: B01J20/22 ,  B01D15/20 ,  B01J20/286 ,  B01J20/287 ,  B01J20/3257 ,  B01J20/3259 ,  G01N30/02

Abstract: This invention provides aqueous-compatible, polar-embedded reversed-phase stationary phase compositions, devices and systems comprising the stationary phases as well as methods of producing these compositions using epoxide ring-opening reactions. Also provided are methods of using the stationary phases of the invention in separations.

Abstract translation: 本发明提供含水相容的极性嵌入式反相固相组合物，包含固定相的装置和体系以及使用环氧化物开环反应制备这些组合物的方法。 还提供了在分离中使用本发明的固定相的方法。

公开(公告)号：US20150224473A1

公开(公告)日：2015-08-13

申请号：US14175689

申请日：2014-02-07

Applicant: THERMO ELECTRON MANUFACTURING LIMITED ,  DIONEX CORPORATION

Inventor： Kevin SKINLEY ,  Xiaodong LIU ,  Christopher A. POHL ,  Harald RITCHIE

IPC: B01J20/283 ,  B01J20/22 ,  B01J20/30 ,  B01J20/28 ,  B01J20/10

CPC classification number: B01J20/283 ,  B01J20/103 ,  B01J20/22 ,  B01J20/28004 ,  B01J20/28016 ,  B01J20/28019 ,  B01J20/28057 ,  B01J20/28059 ,  B01J20/28061 ,  B01J20/28078 ,  B01J20/28083 ,  B01J20/28085 ,  B01J20/287 ,  B01J20/3071 ,  B01J20/3078 ,  B01J20/3085 ,  B01J2220/42 ,  B01J2220/44

Abstract: A particulate material for chromatographic use comprising silica particles is provided having a skeleton structure containing silsesquioxane cage moieties. The material is useful as a chromatographic material, for example in HPLC. The silica particles may be hybrid organo-silica particles wherein the silsesquioxane moieties comprise a cage structure having silicon atoms positioned at corners of the cage wherein one or more silicon atoms positioned at the corners of the cage carry an organic group. A preferred method of preparing the particulate material comprises hydrolysing a silsesquioxane as a co-component of a hydrolysis mixture, especially in a Stöber or modified Stöber process.

Abstract translation: 提供了包含二氧化硅颗粒的用于色谱用途的颗粒材料，其具有含有倍半硅氧烷笼部分的骨架结构。 该材料可用作色谱材料，例如在HPLC中。 二氧化硅颗粒可以是杂化有机二氧化硅颗粒，其中倍半硅氧烷部分包含具有位于笼的拐角处的硅原子的笼状结构，其中位于笼的拐角处的一个或多个硅原子携带有机基团。 制备颗粒材料的优选方法包括水解作为水解混合物的共组分的倍半硅氧烷，特别是在Stöber或改性Stöber方法中。

公开(公告)号：US09029517B2

公开(公告)日：2015-05-12

申请号：US13191992

申请日：2011-07-27

Applicant: David Yavorsky ,  John Amara ,  Joaquin Umana ,  William Cataldo ,  Mikhail Kozlov ,  Matthew Stone

Inventor： David Yavorsky ,  John Amara ,  Joaquin Umana ,  William Cataldo ,  Mikhail Kozlov ,  Matthew Stone

IPC: B01J20/28 ,  B01J47/12 ,  C07K1/18 ,  C07K1/20 ,  C07K1/22 ,  B01D15/32 ,  B01D15/36 ,  B01D15/38 ,  B01J20/285 ,  B01J20/287 ,  B01J20/32 ,  B01J39/26 ,  B01J41/20 ,  B01J47/00

CPC classification number: B01J39/26 ,  B01D15/327 ,  B01D15/361 ,  B01D15/362 ,  B01D15/363 ,  B01D15/3804 ,  B01J20/28023 ,  B01J20/28059 ,  B01J20/285 ,  B01J20/287 ,  B01J20/3244 ,  B01J41/20 ,  B01J47/014 ,  B01J47/127 ,  C07K1/18 ,  C07K16/00 ,  C12N7/00 ,  C12N7/02 ,  G01N2030/521 ,  Y10S502/52724 ,  Y10T428/2938 ,  Y10T428/2973

Abstract: Adsorptive media for chromatography, particularly ion-exchange chromatography, derived from a shaped fiber. In certain embodiments, the functionalized shaped fiber presents a fibrillated or ridged structure which greatly increases the surface area of the fibers when compared to ordinary fibers. Also disclosed herein is a method to add surface pendant functional groups that provides cation-exchange or anion-exchange functionality to the high surface area fibers. This pendant functionality is useful for the ion-exchange chromatographic purification of biomolecules, such as monoclonal antibodies (mAbs).

Abstract translation: 来自成形纤维的用于色谱的吸附介质，特别是离子交换层析。 在某些实施方案中，与普通纤维相比，官能化成形纤维呈现原纤化或脊状结构，其大大增加了纤维的表面积。 本文还公开了向高表面积纤维添加提供阳离子交换或阴离子交换官能团的表面侧基官能团的方法。 这种侧链官能团可用于生物分子的离子交换色谱纯化，如单克隆抗体（mAb）。

公开(公告)号：US08968567B2

公开(公告)日：2015-03-03

申请号：US12388047

申请日：2009-02-18

Applicant: Narushi Ito ,  Jun Kimura ,  Masayuki Mizushina ,  Yohei Inaba ,  Kenji Yokoyama

Inventor： Narushi Ito ,  Jun Kimura ,  Masayuki Mizushina ,  Yohei Inaba ,  Kenji Yokoyama

IPC: B01D15/32 ,  B01J20/28 ,  B01J20/287 ,  B01D15/36

CPC classification number: B01J20/287 ,  B01D15/325 ,  B01D15/362 ,  B01J20/28004

Abstract: Aimed at readily and exactly separate 8-hydroxy-2′-deoxyguanosine (8-OHdG) contained in a sample, a column packing material used for separating 8-OHdG, which contains a packing material composed of a material having a straight-chain hydrocarbon group having 6 or more and 30 or less carbon atoms as a functional group, and having a carbon content over the surface of carrier of 18% or less by element ratio, wherein the packing material contains 1 cumulative percent or more and 20 cumulative percent or less, on the particle-count basis, of particles having a circle-equivalent diameter, measured using a flow-type particle image analyzer, of 0.5 μm or larger and 10 μm or smaller, is used.

Abstract translation: 针对样品中所含的8-羟基-2'-脱氧鸟苷（8-OHdG）容易且精确地分离，用于分离8-OHdG的柱填充材料，其包含由具有直链烃的材料组成的包装材料 具有6个或更多和30个或更少碳原子的官能团，并且在载体表面上的碳含量超过18％或更少的元素比，其中包装材料包含1个累积百分比或更多和20个累积百分比，或 使用0.5μm以上且10μm以下的使用流动型粒子图像分析仪测定的具有圆当量直径的粒子，以粒子计数为基准。