公开(公告)号：US08575324B2

公开(公告)日：2013-11-05

申请号：US11645988

申请日：2006-12-27

申请人： Renu B. Lal ,  Danuta Pieniazek ,  Chunfu Yang

发明人： Renu B. Lal ,  Danuta Pieniazek ,  Chunfu Yang

IPC分类号： C07H21/04 ,  C12Q1/04 ,  C12Q1/68 ,  C12Q1/70

CPC分类号： C12Q1/6883 ,  C12Q1/703 ,  C12Q2549/119

摘要： Reagents and assays for detecting HIV-1 groups M and O and optionally HIV-1 group N and SIVcpz are provided. The reagents are nucleic acid primers for the hybridization to, amplification and subsequent detection of HIV-1 groups M, N and O and SIVcpz in a biological sample. The primers are oligonucleotides that selectively hybridize to the highly conserved regions of the env and pol regions of HIV-1. Due to the high sensitivity of the assays, small concentrations of HIV in a biological sample can be detected, allowing diagnosis at an early stage of infection. The assays are qualitative or quantitative and are useful for viral load determinations of HIV-1 groups M, N or O in a patient undergoing treatment for HIV-1 infection. Viral load determinations can be used to monitor the progress of the treatment regimen, the development of drug resistance, and to predict disease progression.

摘要翻译： 提供了用于检测HIV-1组M和O以及任选的HIV-1组N和SIVcpz的试剂和测定法。 试剂是用于杂交，扩增和随后检测生物样品中HIV-1组M，N和O和SIVcpz的核酸引物。 引物是与HIV-1的env和pol区域的高度保守区域选择性杂交的寡核苷酸。 由于测定的高灵敏度，可以检测生物样品中的小浓度的HIV，从而允许在感染早期进行诊断。 测定是定性的或定量的，并且可用于经历HIV-1感染治疗的患者中HIV-1组M，N或O的病毒载量测定。 病毒载量测定可用于监测治疗方案的进展，耐药性的发展以及预测疾病进展。

公开(公告)号：US5378805A

公开(公告)日：1995-01-03

申请号：US574352

申请日：1990-08-29

申请人： Renu B. Lal

发明人： Renu B. Lal

IPC分类号： A61K39/00 ,  C07K14/15 ,  C07K7/08

CPC分类号： C07K14/005 ,  A61K39/00 ,  C12N2740/14022 ,  Y10S530/826

摘要： The present invention relates a peptide having specific immunoreactivity to antibodies to HTLV-I, HTLV-II, or combinations thereof comprising a peptide selected from the group consisting of:Env-1 (HTLV-I; a.a 191-214)LPHSNLDHILEPSIPWKSKLLTLV,Env-2 (HTLV-II; a.a 187-210)VHDSDLEHVLTPSTSWTTKILKFI,Env-5 (HTLV-I; a.a 242-257)SPNVSVPSSSSTPLLY,Gag-1a (HTLV-I; a.a 102-117)PPSSPTHDPPDSDPQI,Pol-3 (HTLV-I; a.a 487-502)KQILSQRSFPLPPPHK, andanalogues thereof, wherein the amino acids in the sequence may be substituted as long as the immunoreactivity to antibodies to HTLV-I or HTLV-II derived from the three dimensional conformation of the sequences is substantially preserved.The invention is further directed to an immunoassay method for the detection of antibodies to HTLV-I, HTLV-II or a combination thereof, a test kit for the detection of said antibodies, a peptide composition containing said peptides and a vaccine.

摘要翻译： 本发明涉及具有与HTLV-1，HTLV-II的抗体或其组合具有特异性免疫反应性的肽，其包含选自以下的肽：Env-1（HTLV-1; aa 191-214）LPHSNLDHILEPSIPWKSKLLTLV， 2（HTLV-II; aa 187-210）VHDSDLEHVLTPSTSWTTKILKFI，Env-5（HTLV-1; aa 242-257）SPNVSVPSSSSTPLLY，Gag-1a（HTLV-1; aa 102-117）PPSSPTHDPPDSDPQI，Pol-3（HTLV-1 ; aa 487-502）KQILSQRSFPLPPPHK及其类似物，其中序列中的氨基酸可以被取代，只要从序列的三维构象衍生的对HTLV-1或HTLV-II的抗体的免疫反应性被基本上保持。 本发明还涉及用于检测HTLV-1，HTLV-II或其组合的抗体的免疫测定方法，用于检测所述抗体的检测试剂盒，含有所述肽的肽组合物和疫苗。

公开(公告)号：US5420244A

公开(公告)日：1995-05-30

申请号：US103742

申请日：1993-08-06

申请人： Donna L. Rudolph ,  Renu B. Lal

发明人： Donna L. Rudolph ,  Renu B. Lal

IPC分类号： C07K14/16 ,  A61K38/00

CPC分类号： C07K14/005 ,  C12N2740/16322

摘要： The invention provides antigenic peptides derived from immunodominant epitopes of the HTLV-I tax or rex proteins that are immunoreactive with antibodies associated with disease in HTLV-I infected subjects. More specifically, the invention provides antigenic peptides consisting essentially of the amino acid sequences defined in the sequence listing by SEQ ID NOs: 1, 2, 3, 4, 5, 6, 7 and 8 and antigenic fragments thereof. The invention provides a method of diagnosing HTLV-I associated myelopathy (HAM) or a predisposition thereto, comprising the steps of: (a) contacting an antibody containing sample from the subject with a detectable amount of a peptide of the invention or an antigenic fragment thereof; and (b) detecting the reaction of the peptide with an antibody in the sample, the reaction indicating HTLV-I associated myelopathy or a predisposition thereto. The invention also provides a method of diagnosing adult T-cell leukemia or a predisposition thereto in a subject, comprising the steps of: (a) contacting an antibody containing sample from the subject with a detectable amount of the peptide of SEQ ID NO: 1; and (b) detecting the reaction of the peptide with an antibody in the sample, the reaction indicating adult T-cell leukemia or predisposition thereto.

摘要翻译： 本发明提供了衍生自HTLV-1的免疫显性表位的抗原肽，或与HTLV-1感染的受试者中与疾病相关的抗体免疫反应的rex蛋白。 更具体地，本发明提供了基本上由SEQ ID NO：1,2,3,4,5,6,7和8的序列表中定义的氨基酸序列及其抗原性片段组成的抗原肽。 本发明提供了诊断HTLV-1相关性脊髓病（HAM）或其倾向的方法，其包括以下步骤：（a）使来自受试者的抗体含有可检测量的本发明的肽或抗原性片段 的; 和（b）检测样品中肽与抗体的反应，表明HTLV-1相关的脊髓病或其倾向的反应。 本发明还提供了一种在受试者中诊断成人T细胞白血病或倾向于其的方法，包括以下步骤：（a）使来自受试者的含抗体的样品与可检测量的SEQ ID NO：1的肽接触 ; 和（b）检测样品中肽与抗体的反应，该反应指示成年T细胞白血病或其倾向。

公开(公告)号：US07425611B2

公开(公告)日：2008-09-16

申请号：US10550651

申请日：2004-03-26

申请人： Renu B. Lal ,  Sherry M. Owen

发明人： Renu B. Lal ,  Sherry M. Owen

IPC分类号： C07K5/00 ,  A61K39/00 ,  C07H21/04

CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K39/12 ,  A61K39/21 ,  A61K2039/53 ,  A61K2039/57 ,  A61K2039/645 ,  C07K2319/01 ,  C07K2319/06 ,  C12N2740/16034 ,  C12N2740/16122 ,  C12N2740/16222 ,  C12N2740/16322

摘要： Described herein are nucleic acid molecules which encode multiple highly conserved epitopes from HIV-1 proteins, and optionally also epitopes from CCR5; usually also included sequences that encode spacers between two or more of the epitopes. Some of the provided nucleic acid molecules further include sequences that encode targeting domains, useful for targeting the encoded protein into a pathway for enhancing epitope presentation in a vertebrate immune system. Also described are multivalent proteins encoded for by these nucleic acid molecules. The disclosure also encompasses immunogenic compositions that comprise one or more of the nucleic acid molecules, and/or one or more of the proteins encoded thereby, as well as methods of inducing an immune response against HIV-1 in a subject by administering to the subject an effective amount of a composition containing one or more of these molecules. Also provided are cultured host cells containing within them one or more of the described nucleic acid molecules.

摘要翻译： 本文描述的是编码来自HIV-1蛋白质的多个高度保守的表位的核酸分子，以及任选地还包含来自CCR5的表位的核酸分子; 通常还包括在两个或多个表位之间编码间隔区的序列。 一些所提供的核酸分子还包括编码靶向结构域的序列，其可用于将编码的蛋白质靶向用于增强脊椎动物免疫系统中的表位呈递的途径。 还描述了由这些核酸分子编码的多价蛋白质。 本公开还包括包含一个或多个核酸分子和/或由其编码的一种或多种蛋白质的免疫原性组合物，以及通过施用于受试者在受试者中诱导针对HIV-1的免疫应答的方法 有效量的含有这些分子中的一种或多种的组合物。 还提供了在其中含有一种或多种所述核酸分子的培养的宿主细胞。