公开(公告)号：US20230235263A1

公开(公告)日：2023-07-27

申请号：US18098926

申请日：2023-01-19

Applicant: Repligen Corporation

Inventor： Shashi Kudugunti ,  Earl Pineda ,  Michael Bransby

IPC: C12M1/00 ,  C12M1/26

CPC classification number: C12M29/10 ,  C12M29/04 ,  C12M33/14 ,  C12M47/04

Abstract: A method of improving volumetric productivity from a cell culture includes filtering a cell culture through an ultrafilter or a microfilter operating in tangential flow filtration mode or alternating flow filtration mode, and filtering the cell culture concurrently or intermittently through a tangential flow depth filtration system to remove cellular debris and/or to harvest cell product. A system for filtering biological materials includes a primary filtration system, and a secondary filtration system, where the secondary filtration system comprises a tangential flow depth filtration filter.

公开(公告)号：US20230383241A1

公开(公告)日：2023-11-30

申请号：US18200112

申请日：2023-05-22

Applicant: Repligen Corporation

Inventor： Charles Hill ,  Joey Tse ,  Mario Sinani ,  Earl Pineda

IPC: C12M1/00

CPC classification number: C12M47/10 ,  C12M29/10

Abstract: A bleed recovery system for use in a bioreactor system is disclosed. The bleed recovery system being configured to recover product of interest from the bleed material of the bioreactor system. In one embodiment, the bioreactor system includes a bleed recovery system for removing cell cultures from the bioreactor. The bleed recovery system includes a bleed pump coupled to the bioreactor so that cell bleed material can be removed from the bioreactor, a bleed vessel for receiving the cell bleed material, a cell retention device coupled to the bleed vessel, a harvest pump coupled to the cell retention device to deposit the product of interest into a second harvest tank, and a bleed pump connected to the bleed vessel to deposit the spent bleed material into a waste tank. Thus arranged, some or all of the product of interest can be separated from the spent medium.