公开(公告)号：US06228344B1

公开(公告)日：2001-05-08

申请号：US09258148

申请日：1999-02-26

申请人： Richard Bradley Dorshow ,  Samuel Achilefu ,  Raghavan Rajagopalan ,  Joseph Edward Bugaj

发明人： Richard Bradley Dorshow ,  Samuel Achilefu ,  Raghavan Rajagopalan ,  Joseph Edward Bugaj

IPC分类号： A61K4900

CPC分类号： A61K49/0034 ,  A61K49/0021 ,  A61K49/0043 ,  A61K49/0054 ,  A61K49/0056

摘要： A method of measuring physiological function of a group of body cells, includes the step of selecting a detectable agent capable of emitting a measurable electromagnetic emission. The agent is introduced into body fluid wich contacts the group of body cells. The emission is measured, and physiological function is determined based on measurement of the emission.

摘要翻译： 测量一组身体细胞的生理功能的方法包括选择能够发射可测量的电磁发射的可检测试剂的步骤。 该药剂被引入与身体细胞组接触的体液中。 测量发射，并根据发射的测量确定生理功能。

公开(公告)号：US06217848B1

公开(公告)日：2001-04-17

申请号：US09325769

申请日：1999-06-04

申请人： Samuel Achilefu ,  Richard Bradley Dorshow ,  Joseph Edward Bugaj ,  Raghavan Rajagopalan

发明人： Samuel Achilefu ,  Richard Bradley Dorshow ,  Joseph Edward Bugaj ,  Raghavan Rajagopalan

IPC分类号： A61K5100

CPC分类号： A61K41/0057 ,  A61K41/00 ,  A61K41/0028 ,  A61K49/0013

摘要： Dye-peptide conjugates useful for diagnostic imaging and therapy are disclosed. The dye-peptide conjugates include several cyanine dyes with a variety of bis- and tetrakis(carboxylic acid) homologues. The small size of the compounds allows more favorable delivery to tumor cells as compared to larger molecular weight imaging agents. The various dyes are useful over the range of 350-1300 nm, the exact range being dependent upon the particular dye. Use of dimethylsulfoxide helps to maintain the fluorescence of the compounds. The molecules of the invention are useful for diagnostic imaging and therapy, in endoscopic applications for the detection of tumors and other abnormalities and for localized therapy, for photoacoustic tumor imaging, detection and therapy, and for sonofluorescence tumor imaging, detection and therapy.

摘要翻译： 公开了用于诊断成像和治疗的染料 - 肽缀合物。 染料 - 肽缀合物包括具有多种双和四（羧酸）同系物的几种花青染料。 与较大的分子量成像剂相比，化合物的小尺寸允许更有利于肿瘤细胞的递送。 各种染料在350-1300nm的范围内是有用的，确切的范围取决于特定的染料。 使用二甲基亚砜有助于维持化合物的荧光。 本发明的分子可用于诊断成像和治疗，用于检测肿瘤和其他异常以及用于局部治疗，用于光声肿瘤成像，检测和治疗以及用于声发射肿瘤成像，检测和治疗的内窥镜应用中。

公开(公告)号：US06423547B1

公开(公告)日：2002-07-23

申请号：US09646765

申请日：2000-09-21

申请人： Raghavan Rajagopalan ,  Joseph E. Bugaj ,  Richard Bradley Dorshow ,  Samuel I. Achilefu

发明人： Raghavan Rajagopalan ,  Joseph E. Bugaj ,  Richard Bradley Dorshow ,  Samuel I. Achilefu

IPC分类号： G01N33531

CPC分类号： A61K49/0034 ,  A61K41/0057 ,  A61K49/0021 ,  A61K49/0032 ,  A61K49/0052 ,  A61K49/0054 ,  A61K49/0056

摘要： A composition comprising non-covalent carrier-hapten bioconjugates having the formula: HM-CM wherein HM is a hapten molecule whose molecular weight is generally, but not always, less than 1000 Daltons and is capable of performing specific functions; CM is a carrier molecule, whose molecular weight is generally, but not always, more than 1000 Daltons and is capable of transporting the hapten to a specific site; and the dashed line is a non-covalent bond between the carrier molecule and the hapten molecule. Preferably, the bioconjugates are formed from fluorescent dye haptens such as cyanine, indocyanine, squaraine, porphyrrins, Rose Bengal, and methylene blue dye and carrier molecules such as methylated serum albumin, polyarginine, polyaspartic acid, polyglutamic acid, cyclodextrin, and inulin. The bioconjugates are useful in diagnostic and therapeutic medical procedures because they are stable in vitro before being used and stable in vivo during and after use.

摘要翻译： 包含具有下式的非共价载体 - 半抗原生物缀合物的组合物：其中HM是其分子量通常但不总是小于1000道尔顿并且能够执行特定功能的半抗原分子; CM是一种载体分子，其分子量通常但不总是大于1000道尔顿，并且能够将半抗原转运到特定位点; 并且虚线是载体分子和半抗原分子之间的非共价键。优选地，生物缀合物由荧光染料半抗原如花青，吲哚菁，方酸，卟啉，玫瑰红和亚甲基蓝染料以及载体分子形成 作为甲基化血清白蛋白，聚精氨酸，聚天冬氨酸，聚谷氨酸，环糊精和菊粉。生物缀合物在诊断和治疗医疗过程中是有用的，因为它们在使用前和使用后和使用后在体内稳定，在体内稳定。