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    Dry powder cell culture products containing lipid and methods of production thereof
    1.
    发明申请
    Dry powder cell culture products containing lipid and methods of production thereof 审中-公开

    公开(公告)号：US20060270038A1

    公开(公告)日：2006-11-30

    申请号：US11434513

    申请日：2006-05-16

    申请人： Richard Fike ,  Robert Radominski ,  Barbara Dadey ,  Richard Hassett

    发明人： Richard Fike ,  Robert Radominski ,  Barbara Dadey ,  Richard Hassett

    IPC分类号： C12N5/00 ,  C12N5/02

    CPC分类号： C12N5/0018 ,  C12N2500/36 ,  C12N2500/60

    摘要： The present invention relates generally to nutritive medium, medium supplement, media subgroup and buffer formulations that contain lipid. Specifically, the present invention provides powder nutritive medium, medium supplement and medium subgroup formulations, particularly cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising all of the necessary nutritive factors including lipid components that facilitate the in vitro cultivation of cells. The invention is particularly directed to methods of production of these media, media supplement, media subgroup and buffer formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells, particularly bacterial cells, yeast cells, plant cells and animal cells (including human cells) using these dry powder nutritive media, media supplement, media subgroup and buffer formulations. The invention also relates to methods of producing sterile powdered media, media supplement (particularly powdered sera such as powdered FBS, powdered transferrin, powdered insulin, powdered organ extracts (such as bovine brain or pituitary extracts), powdered growth factors (such as EGF, FGF, etc.) and the like), media subgroup and buffer formulations. In a particularly preferred aspect, the invention relates to such methods wherein the sterilization is accomplished by gamma irradiation. The invention also relates to media, media supplement, media subgroup and buffer powders produced by these methods.

    Dry powder cells and cell culture reagents and methods of production thereof
    3.
    发明申请
    Dry powder cells and cell culture reagents and methods of production thereof 审中-公开
    干粉细胞和细胞培养试剂及其生产方法

    公开(公告)号：US20060275886A1

    公开(公告)日：2006-12-07

    申请号：US11502546

    申请日：2006-08-11

    申请人： Richard Fike ,  Richard Hassett ,  Barbara Dadey ,  Robert Radominski

    发明人： Richard Fike ,  Richard Hassett ,  Barbara Dadey ,  Robert Radominski

    IPC分类号： C12N1/20 ,  C12N1/18 ,  C12N5/04

    CPC分类号： C12N5/0018 ,  C12N1/00 ,  C12N2500/36 ,  C12N2500/60

    摘要： The present invention relates generally to nutritive (e.g., cell culture) medium, medium supplement, media subgroup and buffer formulations. Specifically, the present invention provides powdered nutritive medium, medium supplement and medium subgroup formulations, particularly cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides such powdered formulations that produce particular or desired final ionic and/or pH conditions upon reconstitution with a solvent. The invention is also directed to methods of production of these formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells using these formulations. The invention also relates to methods of producing sterile formulations, and to methods for producing dry cell powders. The invention also provides cell, media, media supplement, media subgroup and buffer powders produced by the methods of the invention.

    摘要翻译： 本发明一般涉及营养（例如，细胞培养）培养基，培养基补充剂，培养基亚组和缓冲液制剂。 具体而言，本发明提供粉状营养培养基，中等补充剂和中等亚组分制剂，特别是细胞培养基补充剂（包括粉末状血清如粉末胎牛血清（FBS）），培养基亚组制剂和细胞培养基，其包含促进 体外培养细胞。 本发明还提供了这样的粉末制剂，其在用溶剂重构时产生特定或期望的最终离子和/或pH条件。 本发明还涉及这些制剂的生产方法，并且还提供使用这些制剂培养原核和真核细胞的试剂盒和方法。 本发明还涉及生产无菌制剂的方法，以及生产干细胞粉末的方法。 本发明还提供了通过本发明的方法生产的细胞，培养基，培养基，培养基和缓冲粉。