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    Dry powder cell culture products containing lipid and methods of production thereof
    1.
    发明申请
    Dry powder cell culture products containing lipid and methods of production thereof 审中-公开

    公开(公告)号：US20060270038A1

    公开(公告)日：2006-11-30

    申请号：US11434513

    申请日：2006-05-16

    申请人： Richard Fike ,  Robert Radominski ,  Barbara Dadey ,  Richard Hassett

    发明人： Richard Fike ,  Robert Radominski ,  Barbara Dadey ,  Richard Hassett

    IPC分类号： C12N5/00 ,  C12N5/02

    CPC分类号： C12N5/0018 ,  C12N2500/36 ,  C12N2500/60

    摘要： The present invention relates generally to nutritive medium, medium supplement, media subgroup and buffer formulations that contain lipid. Specifically, the present invention provides powder nutritive medium, medium supplement and medium subgroup formulations, particularly cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising all of the necessary nutritive factors including lipid components that facilitate the in vitro cultivation of cells. The invention is particularly directed to methods of production of these media, media supplement, media subgroup and buffer formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells, particularly bacterial cells, yeast cells, plant cells and animal cells (including human cells) using these dry powder nutritive media, media supplement, media subgroup and buffer formulations. The invention also relates to methods of producing sterile powdered media, media supplement (particularly powdered sera such as powdered FBS, powdered transferrin, powdered insulin, powdered organ extracts (such as bovine brain or pituitary extracts), powdered growth factors (such as EGF, FGF, etc.) and the like), media subgroup and buffer formulations. In a particularly preferred aspect, the invention relates to such methods wherein the sterilization is accomplished by gamma irradiation. The invention also relates to media, media supplement, media subgroup and buffer powders produced by these methods.

    Dry powder cells and cell culture reagents and methods of production thereof
    3.
    发明申请
    Dry powder cells and cell culture reagents and methods of production thereof 审中-公开
    干粉细胞和细胞培养试剂及其生产方法

    公开(公告)号：US20060275886A1

    公开(公告)日：2006-12-07

    申请号：US11502546

    申请日：2006-08-11

    申请人： Richard Fike ,  Richard Hassett ,  Barbara Dadey ,  Robert Radominski

    发明人： Richard Fike ,  Richard Hassett ,  Barbara Dadey ,  Robert Radominski

    IPC分类号： C12N1/20 ,  C12N1/18 ,  C12N5/04

    CPC分类号： C12N5/0018 ,  C12N1/00 ,  C12N2500/36 ,  C12N2500/60

    摘要： The present invention relates generally to nutritive (e.g., cell culture) medium, medium supplement, media subgroup and buffer formulations. Specifically, the present invention provides powdered nutritive medium, medium supplement and medium subgroup formulations, particularly cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides such powdered formulations that produce particular or desired final ionic and/or pH conditions upon reconstitution with a solvent. The invention is also directed to methods of production of these formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells using these formulations. The invention also relates to methods of producing sterile formulations, and to methods for producing dry cell powders. The invention also provides cell, media, media supplement, media subgroup and buffer powders produced by the methods of the invention.

    摘要翻译： 本发明一般涉及营养（例如，细胞培养）培养基，培养基补充剂，培养基亚组和缓冲液制剂。 具体而言，本发明提供粉状营养培养基，中等补充剂和中等亚组分制剂，特别是细胞培养基补充剂（包括粉末状血清如粉末胎牛血清（FBS）），培养基亚组制剂和细胞培养基，其包含促进 体外培养细胞。 本发明还提供了这样的粉末制剂，其在用溶剂重构时产生特定或期望的最终离子和/或pH条件。 本发明还涉及这些制剂的生产方法，并且还提供使用这些制剂培养原核和真核细胞的试剂盒和方法。 本发明还涉及生产无菌制剂的方法，以及生产干细胞粉末的方法。 本发明还提供了通过本发明的方法生产的细胞，培养基，培养基，培养基和缓冲粉。

    Adhering Surfaces
    5.
    发明申请
    Adhering Surfaces 审中-公开
    贴面

    公开(公告)号：US20080299601A1

    公开(公告)日：2008-12-04

    申请号：US12047997

    申请日：2008-03-13

    申请人： Richard Fike ,  Robert Burrier

    发明人： Richard Fike ,  Robert Burrier

    IPC分类号： C12Q1/02 ,  C12N5/06

    CPC分类号： C12N5/0068 ,  C12M23/20 ,  C12N2533/30 ,  C12N2533/74

    摘要： The present invention relates, in part, to compositions useful for cell culture having one surface in adherence to another surface, e.g., a cell culture matrix in adherence to a surface. The present invention also relates, in part, to methods of adhering one surface to another surface, e.g., adhering a cell culture matrix to a surface, and compositions relating to such methods. The present invention also provides in part, methods for adhering a cell to a surface. Related methods are also provided for determining the effect of at least one compound on a cell(s).

    摘要翻译： 本发明部分地涉及可用于细胞培养物的组合物，所述组合物具有粘附于另一表面的一个表面，例如粘附于表面的细胞培养基质。 本发明还部分地涉及将一个表面粘附到另一个表面的方法，例如将细胞培养基质粘附到表面上，以及与这些方法相关的组合物。 本发明还部分提供了将电池附着在表面上的方法。 还提供了相关方法用于确定至少一种化合物对细胞的影响。

    Compositions and methods for stabilizing susceptible compounds
    7.
    发明授权
    Compositions and methods for stabilizing susceptible compounds 审中-公开

    公开(公告)号：US10669522B2

    公开(公告)日：2020-06-02

    申请号：US13984263

    申请日：2012-02-07

    申请人： Richard Fike ,  Bruce Branchaud ,  Shawn Barrett

    发明人： Richard Fike ,  Bruce Branchaud ,  Shawn Barrett

    IPC分类号： C12N5/00

    摘要： Methods for increasing the stability of, or protecting, labile components, such as ethanolamine, growth factors, vitamins, etc., in compositions such as a cell culture medium, are described. Stability of the labile compound is increased either, by derivatization or by sequestering the labile compound. Sequestering can be done either by encapsulation within a microcapsule or by the use of sequestering agents. Encapsulation can provide controlled release of the susceptible compound being protected. These methods may improve or extend the storage conditions, the shipping and handling of media compositions comprising the labile compounds at room temperature rather than at lower temperatures, thereby decreasing shipping costs.

    Dry powder cells and cell culture reagents and methods of production thereof
    9.
    发明授权
    Dry powder cells and cell culture reagents and methods of production thereof 有权
    干粉细胞和细胞培养试剂及其生产方法

    公开(公告)号：US07572632B2

    公开(公告)日：2009-08-11

    申请号：US10685802

    申请日：2003-10-16

    申请人： Richard Fike ,  William Whitford ,  William Biddle

    发明人： Richard Fike ,  William Whitford ,  William Biddle

    IPC分类号： C12N6/00 ,  C12N1/00 ,  C12N1/20 ,  C12N5/00

    CPC分类号： C12N5/0018 ,  C12N1/14 ,  C12N1/16 ,  C12N1/20 ,  C12N5/0025

    摘要： Methods and compositions involving nutritive media, media supplements, media subgroup and buffer formulations are provided. Powder buffer formulations that produce particular ionic and pH conditions upon reconstitution with a solvent are also disclosed. Methods of producing these media, media supplements, media subgroups and buffer formulations as well as kits and methods for cultivation of procaryotic and eukaryotic cells (including human cells) are also provided. Also disclosed are methods of producing sterile powdered media, media supplements, powdered growth factors, media subgroup and buffer formulations. Methods of sterilization include gamma irradiation. Methods for producing dry cell powders where spray-drying a cell suspension may be used are disclosed. Cells, media, media supplements, media subgroups and buffer powders produced by these methods are also disclosed.

    摘要翻译： 提供了涉及营养介质，培养基补充剂，培养基亚组和缓冲液制剂的方法和组合物。 还公开了在用溶剂重构时产生特定离子和pH条件的粉末缓冲剂制剂。 还提供了生产这些培养基，培养基，培养基和缓冲液制剂的方法以及用于培养原核和真核细胞（包括人细胞）的试剂盒和方法。 还公开了生产无菌粉末培养基，培养基补充剂，粉末生长因子，培养基亚组和缓冲液制剂的方法。 灭菌方法包括γ照射。 公开了可以使用喷雾干燥细胞悬浮液的干细胞粉末的制造方法。 还公开了通过这些方法生产的细胞，培养基，培养基，培养基和缓冲粉。

    Method for delivering oxygen to a cell culture medium
    10.
    发明授权
    Method for delivering oxygen to a cell culture medium 失效
    将氧气输送到细胞培养基的方法

    公开(公告)号：US5364784A

    公开(公告)日：1994-11-15

    申请号：US972366

    申请日：1992-11-05

    申请人： Richard Fike ,  James M. Kubiak

    发明人： Richard Fike ,  James M. Kubiak

    IPC分类号： C12M1/04 ,  C12M1/12 ,  C12N5/00

    CPC分类号： C12M29/04 ,  C12M29/24 ,  Y10S435/818

    摘要： An apparatus and method for delivering oxygen to a cell culture medium is disclosed. In one embodiment, the apparatus includes a transfer chamber placed within the cell culture medium. The transfer chamber is adapted to allow the cell culture medium to come in substantial contact with a liquid chemical that is passing through transfer chamber. The apparatus further includes a vessel placed outside the cell culture medium that serves to contain and continually recharge the liquid chemical. The apparatus further includes a plurality of pumps and tubes for circulating the liquid chemical through the transfer chamber and back to the vessel. When the liquid chemical comes into contact with the cell culture medium, oxygen is transferred from the liquid chemical to the cell culture medium.

    摘要翻译： 公开了一种将氧递送至细胞培养基的装置和方法。 在一个实施方案中，该装置包括置于细胞培养基内的转移室。 转移室适于使细胞培养基与通过转移室的液体化学品基本接触。 该装置还包括放置在细胞培养基外部的容器，其用于容纳并持续对液体化学品进行再充电。 该装置还包括多个泵和管，用于使液体化学品循环通过传送室并返回容器。 当液体化学品与细胞培养基接触时，氧气从液体化学品转移到细胞培养基中。