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    Nucleic acid sequences from diabrotica virgifera virgifera LeConte and uses thereof
    6.
    发明申请
    Nucleic acid sequences from diabrotica virgifera virgifera LeConte and uses thereof 有权
    来自紫花苜蓿virgifera virgifera LeConte的核酸序列及其用途

    公开(公告)号：US20070050860A1

    公开(公告)日：2007-03-01

    申请号：US10205189

    申请日：2002-07-24

    申请人： Scott Andersen ,  Glenn Hicks ,  Joseph Huesing ,  Charles Romano ,  Clayton Vetsch

    发明人： Scott Andersen ,  Glenn Hicks ,  Joseph Huesing ,  Charles Romano ,  Clayton Vetsch

    IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N15/82

    CPC分类号： C07K14/43563 ,  C12Q1/6888

    摘要： Expressed Sequence Tags (ESTs) isolated from the Western Corn Rootworm, Diabrotica virgifera virgifera LeConte, are disclosed. The invention encompasses nucleic acid molecules that encode D. v. virgifera protein homologs and fragments thereof. In addition, antibodies capable of binding the proteins are encompassed by the present invention. The disclosed ESTs have particular utility in isolating genes and promoters, identifying and mapping the genes involved in developmental and metabolic pathways, and determining gene function. The ESTs provide a unique molecular tool for the targeting and isolation of novel genes for plant protection and improvement. The invention also relates to methods of using the disclosed nucleic acid molecules, proteins, fragments of proteins, and antibodies, for example, for gene identification and analysis, and preparation of constructs.

    摘要翻译： 公开了从西部玉米根虫，Diabrotica virgifera virgifera LeConte分离的表达序列标签（EST）。 本发明包括编码D.V. virgifera蛋白质同系物及其片段的核酸分子。 此外，本发明涵盖能够结合蛋白质的抗体。 所公开的EST在分离基因和启动子方面具有特别的用途，鉴定和绘制参与发育和代谢途径的基因以及确定基因功能。 EST为靶基因和分离新基因提供了独特的分子工具，用于植物保护和改良。 本发明还涉及使用所公开的核酸分子，蛋白质，蛋白质片段和抗体的方法，例如用于基因鉴定和分析以及构建体的制备。

    Methods for the treatment and prevention of diseases of biological conduits
    9.
    发明申请
    Methods for the treatment and prevention of diseases of biological conduits 审中-公开
    治疗和预防生物管道疾病的方法

    公开(公告)号：US20070141042A1

    公开(公告)日：2007-06-21

    申请号：US10546523

    申请日：2004-02-27

    申请人： Nicholas Franano ,  Charles Romano

    发明人： Nicholas Franano ,  Charles Romano

    IPC分类号： A61K38/48 ,  A61M31/00

    CPC分类号： A61K38/488 ,  A61K38/195 ,  A61K38/4826 ,  A61K38/4833 ,  A61K38/484 ,  A61K38/486 ,  A61K38/4886 ,  A61K2300/00

    摘要： Methods are described for dilating biological conduits by removing elastin and remodeling collagens in the wall of the conduit. Methods include the use of agents that increase the release of endogenous elastase and collagenase in the wall of the conduit, either by cells that are normally present in the wall of the conduit or by inflammatory cells that are attracted to the conduit, thereby providing additional conduit dilation. Methods also include the use of agents that increase conduit wall permeability and expose elastin and collagen fibers. Methods also include removing components of the extracellular matrix of arteries and veins leading to an inhibition of intimal hyperplasia in the wall of the vessels by decreasing biomechanical stimuli directed toward the cells in the wall of the vessel. Methods further include the use of agent that degrade micro fibers, in addition to elastin, in order to decrease the resynthesis of elastin. Methods also include the use of agent that stabilize the diameter of aneurysmal arteries by blocking cell surface receptors in the wall of the aneurysmal artery that are important in the recruitment of inflammatory cells.

    摘要翻译： 描述了通过去除导管壁中的弹性蛋白和重塑胶原来扩张生物管道的方法。 方法包括使用通过存在于管道壁中的细胞或被吸引到导管的炎性细胞来增加导管壁中内源性弹性蛋白酶和胶原酶释放的试剂，从而提供另外的导管 扩张。 方法还包括使用增加导管壁渗透性并暴露弹性蛋白和胶原纤维的试剂。 方法还包括通过减少针对血管壁中的细胞的生物力学刺激来除去动脉和静脉细胞外基质的成分，从而导致血管壁内膜增生的抑制。 方法还包括使用除弹性蛋白之外降解微纤维的试剂，以减少弹性蛋白的再合成。 方法还包括使用通过阻断在炎性细胞募集中重要的动脉瘤动脉壁中的细胞表面受体来稳定动脉瘤动脉直径的药剂。

    Nucleotide sequences encoding cry1bb proteins for enhanced expression in plants
    10.
    发明申请
    Nucleotide sequences encoding cry1bb proteins for enhanced expression in plants 审中-公开
    编码cry1bb蛋白的核苷酸序列用于增强植物中的表达

    公开(公告)号：US20060112447A1

    公开(公告)日：2006-05-25

    申请号：US10525318

    申请日：2003-08-26

    申请人： Natalia Bogdanova ,  Charles Romano

    发明人： Natalia Bogdanova ,  Charles Romano

    IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N5/04 ,  A01H1/00

    CPC分类号： C07K14/325 ,  C12N15/8286 ,  Y02A40/162

    摘要： The present invention describes compositions and methods that are useful in the control of lepidopteran insect pests, and more particularly describes nucleotide sequences for use in plants that encode full-length and truncated insecticidal toxins, as well as chimeric toxins. The nucleotide sequences of the present invention exhibit modifications that, when compared to the native sequences obtained from Bacillus thuringiensis species, make them particularly useful for enhanced, improved, and or optimized expression in monocot and dicot plant species. Using methods well known to those skilled in the art the nucleotide sequences described herein can be used to transform plant cells and plant tissue in order to produce transgenic plants that express the encoded proteins, therefore conferring upon the transgenic plants the ability to resist insect infestation.

    摘要翻译： 本发明描述了可用于控制鳞翅目昆虫害虫的组合物和方法，更具体地描述了用于编码全长和截短的杀虫毒素的植物以及嵌合毒素的核苷酸序列。 本发明的核苷酸序列表现出修饰，当与从苏云金芽孢杆菌属物种获得的天然序列比较时，使它们特别可用于单子叶植物和双子叶植物物种中增强，改良和/或优化的表达。 使用本领域技术人员熟知的方法，本文所述的核苷酸序列可用于转化植物细胞和植物组织，以便产生表达编码蛋白质的转基因植物，从而赋予转基因植物抵抗昆虫侵袭的能力。