-
1.
公开(公告)号:US5231029A
公开(公告)日:1993-07-27
申请号:US793341
申请日:1992-01-14
CPC分类号: G01N1/312 , B01J19/0046 , B01J2219/00351 , B01J2219/00475 , B01J2219/00527 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00619 , B01J2219/0063 , B01J2219/00686 , B01J2219/00689 , B01J2219/00722 , C40B40/06 , C40B60/14 , G01N2001/317 , Y10S435/809 , Y10T436/112499 , Y10T436/113332 , Y10T436/114998 , Y10T436/115831 , Y10T436/25
摘要: Apparatus for the in situ hybridization of slide-mounted cell samples contains modules for the three processes required: (a) reagent metering and spreading on a slide (1) which is effected by drawing a quantity of reagent (56-59) into a reservoir (31) and then spraying it on the slide (1) using air pressure. The rate of flow through the spray is ascertained by measurement and the dosage determined by timing the spraying; (b) slide washing; and (c) slide incubation. The slide washing and incubation are performed in the same dual-purpose housing (16). A high humidity level is maintained in the housing during incubation so that the samples do not need to be covered to prevent them drying out. A plurality of slides (1) are mounted in a carrier (11) to be washed and incubated at the same time.
摘要翻译: PCT No.PCT / GB90 / 01310 Sec。 371日期:1992年1月14日 102(e)日期1992年1月14日PCT提交1990年8月21日PCT公布。 出版物WO91 / 02962 日期:1991年3月7日。载玻片安装的细胞样品的原位杂交装置包含所需三个方法的模块:(a)试剂计量和在载玻片上铺展(1),其通过拉伸一定量的试剂 56-59)放入贮存器(31)中,然后使用空气压力将其喷洒在载玻片(1)上。 通过测量和通过喷涂定时确定的剂量确定通过喷雾的流速; (b)滑洗; 和(c)载玻片孵育。 载玻片洗涤和孵育在相同的两用壳体(16)中进行。 在孵育期间保持壳体内的高湿度水平,使得样品不需要被覆盖以防止它们变干。 将多个载玻片(1)安装在载体(11)中以进行洗涤并同时温育。