公开(公告)号：US07001715B2

公开(公告)日：2006-02-21

申请号：US10090428

申请日：2002-02-28

申请人： Robert A. Houtchens ,  Maria S. Gawryl ,  William R. Light ,  Javed Baqai

发明人： Robert A. Houtchens ,  Maria S. Gawryl ,  William R. Light ,  Javed Baqai

IPC分类号： A01N1/02 ,  A61K38/16 ,  A61K35/14 ,  C07K17/00

CPC分类号： A61K35/18

摘要： Red blood cells are purified by separating whole blood, such as by centrifugation, to form a red blood cell fraction and a liquid fraction. The whole blood can be defibrinated or treated to prevent coagulation prior to separation. Preferably, the whole blood is bovine blood. The red blood cell fraction is then diafiltered to purify the red blood cells. The purified red blood cells can then be lysed to form a lysate of purified red blood cells. The purified red blood cells and the lysate of purified red blood cells are suitable for use in producing hemoglobin blood substitute.

公开(公告)号：US20100209532A1

公开(公告)日：2010-08-19

申请号：US12451997

申请日：2008-06-13

申请人： Gregory P. Dube ,  Zafiris W. Zafirelis ,  Anthony J. Laccetti ,  Javed Baqai

发明人： Gregory P. Dube ,  Zafiris W. Zafirelis ,  Anthony J. Laccetti ,  Javed Baqai

IPC分类号： A61K38/42 ,  A61K33/42 ,  A61K33/06 ,  A61K33/00 ,  A61P9/10

CPC分类号： A61K38/42 ,  A61K9/0026 ,  A61K38/38 ,  B01D61/18 ,  B01D2315/16 ,  A61K2300/00

摘要： A method of delivering oxygen to a tissue, a blood vessel, an organ, or a region of an organ, under an ischemic condition, or prophylactically preventing occurrence of an ischemic condition, of a subject, comprising the step of administering to the subject an oxygenated hemoglobin solution, wherein the oxygenated hemoglobin solution includes polymerized hemoglobin, and wherein about 80% by weight, or greater, of the polymerized hemoglobin is oxyhemoglobin.

摘要翻译： 一种在局部缺血状态下向组织，血管，器官或器官区域输送氧气或预防性地预防局部缺血病症发生的方法，其包括以下步骤：向受试者施用 氧化血红蛋白溶液，其中所述含氧血红蛋白溶液包括聚合血红蛋白，并且其中约80重量％以上的聚合血红蛋白为氧合血红蛋白。

公开(公告)号：US20080069771A1

公开(公告)日：2008-03-20

申请号：US11901462

申请日：2007-09-17

申请人： Anthony Laccetti ,  Javed Baqai

发明人： Anthony Laccetti ,  Javed Baqai

IPC分类号： C07K14/805 ,  A61K49/14 ,  A61K51/08

CPC分类号： C07K14/805 ,  A61B5/0066 ,  A61B5/413 ,  A61K49/14 ,  A61K49/18

摘要： An oxygenated Hemoglobin (Hb) solution includes from about 10 g to about 250 g of polymerized Hb per liter of solution. About 80% by weight, or greater, of the polymerized Hb of the oxygenated hemoglobin solution is oxyhemoglobin. About 18% by weight, or less, of the polymerized Hb has a molecular weight of over 500,000 Daltons. About 5% by weight, or less, of the polymerized Hb has a molecular weight equal to or less than 65,000 Daltons. A P50 of the polymerized Hb is in a range of between about 34 and about 46 mm Hg. An endotoxin content of the oxygenated Hb solution is less than about 0.05 endotoxin units per mL. A method of visualizing a tissue or organ of a subject includes the steps of administering to the subject an oxygenated hemoglobin solution as described above, and imaging the tissue, blood vessel or organ with an imaging system. A method of producing an oxygenated Hb solution includes the step of oxygenating a Hb solution that includes polymerized Hb as described above to thereby cause about 80% by weight, or greater, of the polymerized Hb to become oxyhemoglobin.

摘要翻译： 含氧血红蛋白（Hb）溶液包括每升溶液约10g至约250g聚合的Hb。 约80重量％以上的氧合血红蛋白溶液的聚合Hb为氧合血红蛋白。 约18重量％以下的聚合Hb具有超过500,000道尔顿的分子量。 约5重量％以下的聚合Hb具有等于或小于65,000道尔顿的分子量。 聚合的Hb的P 50 O在约34和约46mm Hg之间的范围内。 含氧化Hb溶液的内毒素含量小于每毫升约0.05内毒素单位。 可视化受试者的组织或器官的方法包括以下步骤：向受试者施用如上所述的氧合血红蛋白溶液，并用成像系统对组织，血管或器官进行成像。 制造含氧化Hb溶液的方法包括如上所述使含有聚合Hb的Hb溶液氧化，从而使聚合的Hb约80重量％以上变成氧合血红蛋白的步骤。