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    Haptens, tracers, immunogens and antibodies for immunoassays for cotinine derivatives
    2.
    发明授权
    Haptens, tracers, immunogens and antibodies for immunoassays for cotinine derivatives 失效
    呼吸器，跟踪器，免疫原和抗体用于免疫调节剂

    公开(公告)号：US5164504A

    公开(公告)日：1992-11-17

    申请号：US641840

    申请日：1991-01-16

    申请人： John A. Walling ,  Hsiang-Yun Hu ,  Barbara E. Hasz

    发明人： John A. Walling ,  Hsiang-Yun Hu ,  Barbara E. Hasz

    IPC分类号： C07D401/04 ,  G01N33/53

    CPC分类号： C07D401/04 ,  G01N33/5308

    摘要： Disclosed is a substantially optically pure hapten, useful in an immunoassay for cotinine. The hapten corresponds to a specified structural formula (I). In formula (I) X is a straight or branched chain, saturated or unsaturated, divalent radical covalently bonded to a pyridyl ring at the number 2-, 4-, 5- or 6-position. The divalent radical, X, has from 1 to 10 carbon atoms wherein the chain of the divalent radical optionally may contain 1 or 2 hetero atoms selected from members of the group consisting of S, O and NZ in which Z represents a C.sub.1 to C.sub.3 alkyl group. In formula (I), n=1 or 0, and Q is a functional group selected from --COOH, --NH.sub.2 (provided that n does not equal 0), --C(O)NHNH.sub.2, --O(CO)Cl, --CHO, --NCS or --NCO.Also disclosed is an immunogen derived from the hapten as well as an antibody raised in response to an immunogen derived from the hapten.Also disclosed is a fluorescent tracer derived from a substantially optically pure compound corresponding to the hapten, the tracer being useful in an immunoassay for cotinine.Also disclosed is an improved immunoassay for determining cotinine in a biological sample involving a step of contacting the sample with antibodies raised in response to the immunogen. Also disclosed is a fluorescence polarization immunoassay (FPIA) for determining cotinine involving a step of contacting the sample with antibodies raised in response to the immunogen, and/or involving a step of contacting the sample with a fluorescent tracer.

    摘要翻译： 公开了一种基本上光学纯的半抗原，其可用于可替宁的免疫测定。 半抗原对应于指定的结构式（I）。 在式（I）中，X是在2-，4-，5-或6-位上共价结合到吡啶环上的直链或支链饱和或不饱和的二价基团。 二价基团X具有1至10个碳原子，其中二价基团的链任选地可以含有1或2个选自S，O和NZ的杂原子，其中Z表示C1至C3烷基 组。 在式（I）中，n = 1或0，Q为选自-COOH，-NH 2（n不等于0）的官能团，-C（O）NHNH 2，-O（CO）Cl - CHO，-NCS或-NCO。 还公开了衍生自半抗原的免疫原以及响应于来自半抗原的免疫原产生的抗体。 还公开了衍生自对应于半抗原的基本上光学上纯的化合物的荧光示踪剂，该示踪剂可用于可替宁的免疫测定。 还公开了用于在生物样品中测定可替宁的改进的免疫测定，其涉及将样品与响应于免疫原产生的抗体接触的步骤。 还公开了用于测定可替宁的荧光偏振免疫测定（FPIA），其涉及将样品与响应于免疫原而产生的抗体接触的步骤和/或涉及使样品与荧光示踪剂接触的步骤。

    Nicotine-containing, controlled release composition and method
    6.
    发明授权
    Nicotine-containing, controlled release composition and method 有权
    含尼古丁，控释组合物和方法

    公开(公告)号：US06828336B2

    公开(公告)日：2004-12-07

    申请号：US10429420

    申请日：2003-05-05

    申请人： John A. Walling

    发明人： John A. Walling

    IPC分类号： A61K31465

    CPC分类号： A61K31/465

    摘要： A nicotine product having a nicotine release rate of not less than 70% over a 10 minute period as well as a process to produce such product. Such product is produced by a process comprising the steps of (a) preparing an aqueous solution of an organic polyol; (b) mixing said aqueous solution of the organic polyol with a cation exchange resin selected from the group consisting of (i)—a methacrylic, weakly acidic type of resin containing carboxylic functional groups (ii)—polystyrene, strongly acidic type of resin containing sulfonic functional groups and (iii)—polystyrene, intermediate acidic type of resin containing phosphonic functional groups thereby forming a cation exchange resin mixture having some of its ion exchange sites partially blocked with said polyol; (c) admixing with said mixture of step (b) an aqueous solution of nicotine to form a nicotine-coated cation exchange resin admixture; and (d) removing water from said admixture. The nicotine composition having a nicotine release rate of not less than 70% over a 10 minute period results.

    摘要翻译： 尼古丁产品在10分钟内具有不小于70％的尼古丁释放速率，以及生产这种产品的方法。 该产品通过包括以下步骤的方法生产：（a）制备有机多元醇的水溶液;（b）将所述有机多元醇的水溶液与选自（i）-a 含有羧基官能团的甲基丙烯酸，弱酸性树脂（ⅱ） - 聚苯乙烯，含有磺酸官能团的强酸型树脂和（ⅲ）聚苯乙烯，含有膦酸官能团的中间体酸型树脂，从而形成阳离子交换树脂混合物， 其一些离子交换位点被所述多元醇部分封闭;（c）与步骤（b）的混合物混合尼古丁水溶液以形成尼古丁涂覆的阳离子交换树脂混合物; 并且（d）从所述混合物中除去水。在10分钟时间内具有不小于70％的尼古丁释放速率的尼古丁组合物。