公开(公告)号：US06627461B2

公开(公告)日：2003-09-30

申请号：US09837898

申请日：2001-04-18

申请人： Robert G. Chapman ,  John Hefti ,  Barrett J. Bartell ,  Mark A. Rhodes ,  Min Zhao ,  Tyler Palmer

发明人： Robert G. Chapman ,  John Hefti ,  Barrett J. Bartell ,  Mark A. Rhodes ,  Min Zhao ,  Tyler Palmer

IPC分类号： G01N33536

CPC分类号： G01N22/00 ,  G01N33/536 ,  G01N2035/00346 ,  G01N2333/75 ,  Y10S436/805 ,  Y10S436/806

摘要： The present invention provides a method for detecting a molecular event, comprising (1) applying an electromagnetic test signal to a sample in which a molecular event is being detected, whereby the sample interacts with and modulates the test signal to produce a modulated test signal, and (2) detecting the modulated test signal, wherein the applying and detecting take place in a temperature-controlled environment, wherein the temperature-controlled environment comprises the sample, a radiating portion of a signal generating circuit, and a receiving portion of a signal detection circuit and wherein the applying and detecting take place in the environment at a temperature controlled to within ±0.5° C.

公开(公告)号：US06485905B2

公开(公告)日：2002-11-26

申请号：US09365978

申请日：1999-08-02

申请人： John Hefti

发明人： John Hefti

IPC分类号： G01N33543

CPC分类号： C12Q1/001 ,  B82Y10/00 ,  G01N33/54373 ,  G01N33/5438 ,  H01J49/04 ,  Y10S436/805 ,  Y10S436/806

摘要： A bio-assay test system includes a test fixture, a measurement system, and a computer. The test fixture includes a bio-assay device having a signal path and a retaining structure configured to place a sample containing molecular structures in electromagnetic communication with the signal path. The measurement system is configured to transmit test signals to and to receive test signals from the signal path at one or more predefined frequencies. The computer is configured to control the transmission and reception of the test signals to and from the measurement system.

摘要翻译： 生物测定测试系统包括测试夹具，测量系统和计算机。 测试夹具包括具有信号路径和保持结构的生物测定装置，所述保持结构被配置为将含有分子结构的样品放置在与信号路径电磁通信中。 测量系统被配置为以一个或多个预定义的频率将测试信号发送到信号路径并从其接收测试信号。 计算机被配置为控制测量信号到测量系统的传输和接收。

公开(公告)号：US06340568B2

公开(公告)日：2002-01-22

申请号：US09839860

申请日：2001-04-19

申请人： John Hefti

发明人： John Hefti

IPC分类号： C12Q168

CPC分类号： B82Y10/00 ,  B82Y30/00 ,  C12Q1/001 ,  C12Q1/6825 ,  G01N33/54373 ,  G01N33/5438 ,  H01J49/04

摘要： The present invention provides a variety of methods for conducting analyses of nucleic acids using a detection system which is sensitive to the unique dielectric properties of different hybridization complexes and which can distinguish between different hybridization complexes directly without the use of labels. Methods using the system to perform sequence checking, expression analysis, de novo sequencing and a variety of other nucleic acid analyses are provided.

摘要翻译： 本发明提供使用对不同杂交复合物的独特介电性能敏感的检测系统进行核酸分析的各种方法，并且可以直接区分不同的杂交复合体而不使用标记。 提供了使用该系统进行序列检查，表达分析，从头测序和多种其他核酸分析的方法。

公开(公告)号：US20050124869A1

公开(公告)日：2005-06-09

申请号：US10728992

申请日：2003-12-08

申请人： John Hefti ,  Joseph Page ,  James Plante

发明人： John Hefti ,  Joseph Page ,  James Plante

IPC分类号： A61B5/00

CPC分类号： A61B5/1455 ,  A61B5/0095 ,  A61B5/14532

摘要： Tissue substance measurement systems are arranged with highly specialized optical sources. Quantum cascade lasers are high power semiconductor lasers which are tiny in size and highly tunable with respect to wavelength. When deployed in non-invasive tissue substance measurement systems, quantum cascade lasers offer system advantages such as high accuracy, small size, convenience, efficiency, among others. These specialized semiconductors may be used with systems based upon photoacoustic principles. Systems may be formed of a plurality of quantum cascade laser in an optical source, mechanism to couple light into tissue, an acoustic detector and a signal processor. In some versions, user interfaces provide a reporting and feedback function to a user.

摘要翻译： 组织物质测量系统配有高度专业化的光源。 量子级联激光器是大功率半导体激光器，其尺寸微小且相对于波长高度可调。 量子级联激光器部署在非侵入性组织物质测量系统中时，提供了高精度，小尺寸，方便，高效等系统优点。 这些专用半导体可以与基于光声原理的系统一起使用。 系统可以由光源中的多个量子级联激光器，将光耦合到组织中的机构，声学检测器和信号处理器来形成。 在某些版本中，用户界面向用户提供报告和反馈功能。

公开(公告)号：US06566079B2

公开(公告)日：2003-05-20

申请号：US09923474

申请日：2001-08-06

申请人： John Hefti

发明人： John Hefti

IPC分类号： G01N3353

CPC分类号： B82Y10/00 ,  B82Y30/00 ,  C12Q1/001 ,  G01N33/54373 ,  G01N33/5438 ,  G01N2333/42 ,  G01N2333/4712 ,  G01N2333/723 ,  G01N2333/924 ,  G01N2333/96486 ,  G01N2333/98 ,  H01J49/04

摘要： The present invention provides a variety of methods of analyzing protein binding events using a system capable of directly detecting protein/ligand complexes based upon the dielectric properties of the complex. The system can be used in a variety of analyses involving protein binding events, such as screening ligand libraries, characterizing protein binding interactions, and identifying ligands. The system can also be utilized in diverse analytical and diagnostic applications.

公开(公告)号：US20090240124A1

公开(公告)日：2009-09-24

申请号：US12406775

申请日：2009-03-18

申请人： John Hefti

发明人： John Hefti

IPC分类号： A61B5/1455

CPC分类号： A61B5/14532

摘要： A method for determining the concentration of an analyte within a volume located between the cornea and the iris of an eye includes determining an index of refraction of a volume disposed between a cornea and an iris of an eye, the volume including an analyte. Based upon the index of refraction of said volume, the concentration of the analyte within the volume is determined.

摘要翻译： 用于确定位于眼睛和眼睛的虹膜之间的体内的分析物的浓度的方法包括确定设置在角膜和眼睛的虹膜之间的体积的折射率，所述体积包括分析物。 基于所述体积的折射率，确定体积内分析物的浓度。

公开(公告)号：US06586946B2

公开(公告)日：2003-07-01

申请号：US09775710

申请日：2001-02-01

申请人： John Hefti ,  Hong Peng

发明人： John Hefti ,  Hong Peng

IPC分类号： G01R2704

CPC分类号： G01N33/487 ,  G01N22/00

摘要： A system and method for detecting a molecular event in a test sample using a resonant test structure is presented. The method includes providing a resonant test structure having a resonant response associated therewith. Next, a first resonant response of the resonant test structure is obtained when the resonant test structure is electromagnetically coupled to a reference sample, the reference sample having a known composition. A second resonant response is also obtained when the resonant test structure is electromagnetically coupled to the test sample, the test sample having an unknown composition. Subsequently, one or more first electrical parameters, such as the q-factor of the resonator, are derived from the first resonant response. One or more second electrical parameters are similarly derived from the second resonant response. The similarity or difference between the first and second electrical parameters are analyzed to determine the presence or absence of the molecular event in the test sample.

公开(公告)号：US06395480B1

公开(公告)日：2002-05-28

申请号：US09243196

申请日：1999-02-01

申请人： John Hefti

发明人： John Hefti

IPC分类号： C12Q168

CPC分类号： G01N33/54373

摘要： Systems and methods for detecting molecular binding events and other environmental effects using the unique dielectric properties of the bound molecular structure or structures are presented. A molecular binding layer is coupled along the surface of a signal path. A test signal is propagated along the signal path, whereby the test signal couples to the molecular binding layer, and in response, exhibits a signal response.

摘要翻译： 提出了使用结合的分子结构或结构的独特介电性质来检测分子结合事件和其他环境影响的系统和方法。 分子结合层沿信号路径的表面耦合。 测试信号沿着信号路径传播，由此测试信号耦合到分子结合层，并且作为响应表现出信号响应。

公开(公告)号：US06338968B1

公开(公告)日：2002-01-15

申请号：US09365578

申请日：1999-08-02

申请人： John Hefti

发明人： John Hefti

IPC分类号： G01N33543

CPC分类号： C12Q1/001 ,  B82Y15/00 ,  B82Y30/00 ,  G01N33/54373 ,  G01N33/5438 ,  Y10S436/805 ,  Y10S436/806

摘要： Systems and methods are presented for detecting molecular binding events and other environmental effects using the unique dielectric properties of the bound molecular structure or structures. A molecular binding region is coupled along the surface of a signal path. A test signal is propagated along the signal path, whereby the test signal couples to the molecular binding region, and in response, exhibits a signal response.

摘要翻译： 提出了使用结合分子结构或结构的独特介电性质来检测分子结合事件和其他环境影响的系统和方法。 分子结合区域沿着信号路径的表面耦合。 测试信号沿着信号路径传播，由此测试信号耦合到分子结合区域，并且作为响应表现出信号响应。

公开(公告)号：US06287874B1

公开(公告)日：2001-09-11

申请号：US09365580

申请日：1999-08-02

申请人： John Hefti

发明人： John Hefti

IPC分类号： G01N33566

CPC分类号： B82Y10/00 ,  B82Y30/00 ,  C12Q1/001 ,  G01N33/54373 ,  G01N33/5438 ,  G01N2333/42 ,  G01N2333/4712 ,  G01N2333/723 ,  G01N2333/924 ,  G01N2333/96486 ,  G01N2333/98 ,  H01J49/04

摘要： The present invention provides a variety of methods of analyzing protein binding events using a system capable of directly detecting protein/ligand complexes based upon the dielectric properties of the complex. The system can be used in a variety of analyses involving protein binding events, such as screening ligand libraries, characterizing protein binding interactions, and identifying ligands. The system can also be utilized in diverse analytical and diagnostic applications.

摘要翻译： 本发明提供了使用能够基于复合物的介电性质直接检测蛋白质/配体复合物的系统来分析蛋白质结合事件的各种方法。 该系统可用于涉及蛋白质结合事件的各种分析，例如筛选配体文库，表征蛋白质结合相互作用以及鉴定配体。 该系统还可用于多种分析和诊断应用。