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    treatment of cardiomyopathy by removal of autoantibodies
    1.
    发明授权
    treatment of cardiomyopathy by removal of autoantibodies 失效
    通过去除自身抗体治疗心肌病

    公开(公告)号：US07022322B2

    公开(公告)日：2006-04-04

    申请号：US10299940

    申请日：2002-11-19

    申请人： Robert Koll ,  Jutta Müller-Derlich ,  Stephan Felix ,  Petra Reinke ,  Stefan Brehme ,  Gert Baumann ,  Reiner Spaethe

    发明人： Robert Koll ,  Jutta Müller-Derlich ,  Stephan Felix ,  Petra Reinke ,  Stefan Brehme ,  Gert Baumann ,  Reiner Spaethe

    IPC分类号： A61K39/395

    CPC分类号： C07K1/22 ,  A61K35/16 ,  B01D15/3809 ,  C07K16/2869 ,  Y10S424/81

    摘要： Immunoapheresis treatment for cardiomyopathy comprises passing the patient's plasma over a column having coupled thereto a specific ligand for human immunoglobulin, thereby removing a significant portion of the immunoglobulin from the patient's plasma, and then reinfusing the plasma to the patient. The invention is the use of a specific ligand for human immunoglobulin in the manufacture of a column having the ligand coupled thereto, the column being useful for immunoapheresis treatment of a patient with cardiomyopathy. The specific ligand binds, and thereby removes, human autoantibodies which are harmful to cardiac tissue such as antibodies against β1-adrenergic receptors, ADP-ATP carriers, α and β myosin heavy chains, and adenine nucleotide translocators. Immunoapheresis treatment using the column results in improvement of hemodynamic parameters such as mean arterial pressure, mean pulmonary pressure, pumonary capillary wedge pressure, right atrial pressure, cardiac output, cariac index, stroke volume index, and systemic vascular resistance.

    摘要翻译： 用于心肌病的免疫分离治疗包括将患者的血浆通过与其连接的用于人免疫球蛋白的特异性配体的柱，从而从患者血浆中除去大部分免疫球蛋白，然后将血浆重新输入患者体内。 本发明是用于人免疫球蛋白的特异性配体用于制备具有偶联配体的柱，该柱可用于对患有心肌病的患者的免疫分离治疗。 特异性配体结合并从而除去对心脏组织有害的人自身抗体，例如针对β1 - 肾上腺素能受体，ADP-ATP载体，α和β肌球蛋白重链的抗体和腺嘌呤核苷酸转运蛋白 。 使用柱的免疫分析法治疗可改善血流动力学参数，如平均动脉压，平均肺压，血管毛细血管楔压，右心房压，心输出量，Cariac指数，卒中体积指数和全身血管阻力。

    ANTIGEN-SPECIFIC CENTRAL-MEMORY T CELL PREPARATIONS HAVING HIGH CD4+ FRACTION
    3.
    发明申请
    ANTIGEN-SPECIFIC CENTRAL-MEMORY T CELL PREPARATIONS HAVING HIGH CD4+ FRACTION 审中-公开
    具有高CD4 +分子的抗原特异性中央记忆细胞制备

    公开(公告)号：US20140154228A1

    公开(公告)日：2014-06-05

    申请号：US14125101

    申请日：2012-06-11

    申请人： Hans-dieter Volk ,  Michael Schmuck ,  Petra Reinke

    发明人： Hans-dieter Volk ,  Michael Schmuck ,  Petra Reinke

    IPC分类号： A61K35/26

    CPC分类号： A61K35/26 ,  A61K35/17 ,  C12N5/0636 ,  C12N2501/2302 ,  C12N2501/2307 ,  C12N2501/727

    摘要： The invention relates to a method for generation of T cell preparations that are specific for at least one target antigen, comprising the steps of expanding lymphoid cells in vitro in the presence of a target antigen or peptide fragments thereof in an expansion step, isolating cells that secrete interferon gamma and culturing, the cells in the presence of interleukin 2 and interleukin 7 and either an inhibitor of the mTOR Complex 1, or in the presence of an inhibitor of IL-2/IL-2R interaction. The invention further relates to preparations obtained by the method of the invention.

    摘要翻译： 本发明涉及一种用于产生对至少一种靶抗原特异性的T细胞制备物的方法，包括以下步骤：在扩增步骤中在靶抗原或其肽片段的存在下体外扩增淋巴样细胞，分离细胞 分泌干扰素γ，并在白细胞介素2和白细胞介素7存在下培养细胞，以及mTOR复合物1的抑制剂，或在IL-2 / IL-2R相互作用的抑制剂存在下培养。 本发明还涉及通过本发明的方法获得的制剂。

    Method for antigen-specific stimulation of T lymphocytes with synthetic peptide libraries
    4.
    发明授权
    Method for antigen-specific stimulation of T lymphocytes with synthetic peptide libraries 有权
    用合成肽文库对T淋巴细胞进行抗原特异性刺激的方法

    公开(公告)号：US07994096B2

    公开(公告)日：2011-08-09

    申请号：US10203915

    申请日：2001-02-17

    申请人： Florian Kern ,  Hans-Dieter Volk ,  Petra Reinke ,  Nicole Faulhaber ,  Ingolf-Pascal Surel ,  Elham Khatamzas

    发明人： Florian Kern ,  Hans-Dieter Volk ,  Petra Reinke ,  Nicole Faulhaber ,  Ingolf-Pascal Surel ,  Elham Khatamzas

    IPC分类号： C40B30/04 ,  C07H81/04

    CPC分类号： G01N33/505

    摘要： The invention relates to a method for the antigen-specific stimulation of T-lymphocytes with a synthetic peptide library, by preparing a plurality of peptides, each of said peptides comprising a fragment less than the whole of the total amino acid sequence of the antigen, each of said peptides being a minimum of 9 amino acid residues (AAs) in length, and the amino acid sequence of each peptide overlapping with the amino acid sequence of at least one other of said peptides; combining a plurality of the peptides from a) into a peptide library, said peptide library comprises a plurality of the peptides selected such that their collective overlapping amino acid sequences span the total amino acid sequence of the antigen; and incubating a suspension comprising CD8+ T lymphocytes, CD4+ T lymphocytes or a mixture of CD8+ and CD4+ with said peptide library in a single culture run.

    摘要翻译： 本发明涉及一种用合成肽文库抗原特异性刺激T淋巴细胞的方法，通过制备多种肽，所述肽中的每一种都包含小于整个抗原总氨基酸序列的片段， 所述肽中的每一个长度为至少9个氨基酸残基（AAs），并且每个肽的氨基酸序列与所述肽中的至少另一个的氨基酸序列重叠; 将来自a）的多个肽组合成肽文库，所述肽文库包含多个选择的肽，使得它们的共同重叠氨基酸序列跨越抗原的总氨基酸序列; 并在单次培养运行中将包含CD8 + T淋巴细胞，CD4 + T淋巴细胞或CD8 +和CD4 +的混合物的悬浮液与所述肽文库一起孵育。

    Method and kit for testing various selected materials and/or surface structures for culturing cells
    6.
    发明申请
    Method and kit for testing various selected materials and/or surface structures for culturing cells 有权
    用于测试培养细胞的各种选择的材料和/或表面结构的方法和试剂盒

    公开(公告)号：US20130053276A1

    公开(公告)日：2013-02-28

    申请号：US13508540

    申请日：2010-09-24

    申请人： Frank Klein ,  Thomas Weigel ,  Karl Kratz ,  Friedrich Jung ,  Bernhard Hiebl ,  Andreas Lendlein ,  Karola Lützow ,  Andreas Kurtz ,  Petra Reinke ,  Andy Römhild

    发明人： Frank Klein ,  Thomas Weigel ,  Karl Kratz ,  Friedrich Jung ,  Bernhard Hiebl ,  Andreas Lendlein ,  Karola Lützow ,  Andreas Kurtz ,  Petra Reinke ,  Andy Römhild

    IPC分类号： C40B30/06 ,  C40B40/00

    CPC分类号： G01N33/5008

    摘要： The present invention relates to a method for testing different selected materials and/or surface structures for the culture of cells and/or microorganisms, said method comprising the following steps: i) providing a plurality of different selected materials and/or surface structures to be tested in the form of beaker-shaped inserts, wherein the beaker-shaped inserts are dimensioned in such a way that a beaker-shaped insert can be inserted in a substantially accurately fitting manner into a well of a multiwell plate suitable for cell culture, wherein each beaker-shaped insert has walls, a base, and an opening in the side facing away from the base, wherein the walls and base of the beaker-shaped inserts are liquid-tight, and wherein each beaker-shaped insert has a selected material and/or a selected surface structure, at least on the inner face of the base; ii) inserting the beaker-shaped inserts into wells of multiwell plates of appropriate dimensioning so that the outer face of the beaker base points towards the inner face of the base of a well of the multiwell plate; iii) culturing the cells in the lumen of the beaker-shaped inserts, and to a kit for carrying out this method.

    摘要翻译： 本发明涉及一种用于测试用于培养细胞和/或微生物的不同选择的材料和/或表面结构的方法，所述方法包括以下步骤：i）提供多种不同的选择的材料和/或表面结构为 以烧杯形插入物的形式进行测试，其中烧杯形插入件的尺寸设计使得可将烧杯形插入件以基本上精确适合的方式插入适合于细胞培养的多孔板的孔中，其中 每个烧杯形插件具有壁，基部和在背离基部的一侧的开口，其中烧杯形插入件的壁和底部是液密的，并且其中每个烧杯形插入件具有选定的材料 和/或选定的表面结构，至少在基底的内表面上; ii）将所述烧杯形插入物插入适合尺寸的多孔板的孔中，使得所述烧杯基底的外表面指向所述多孔板的孔的底部的内表面; iii）在烧杯形插入物的内腔中培养细胞，以及用于实施该方法的试剂盒。