公开(公告)号：US20130022998A1

公开(公告)日：2013-01-24

申请号：US13560080

申请日：2012-07-27

申请人： Robert L. Cheek ,  Eric P. Dixon ,  Timothy J. Fischer ,  John W. Groelke ,  Steven L. Knapp ,  Stephen G. Simkins ,  Clark M. Whitehead

发明人： Robert L. Cheek ,  Eric P. Dixon ,  Timothy J. Fischer ,  John W. Groelke ,  Steven L. Knapp ,  Stephen G. Simkins ,  Clark M. Whitehead

IPC分类号： G01N33/574

CPC分类号： C07K16/3069 ,  C07K16/18 ,  C07K2317/34 ,  G01N33/57449

摘要： Compositions and methods for diagnosing ovarian cancer in a patient and for identifying patients with an increased likelihood of having ovarian cancer are provided. The compositions include novel monoclonal antibodies, and variants and fragments thereof, that specifically bind to glycodelin. Monoclonal antibodies having the binding characteristics of a glycodelin antibody of the invention and monoclonal antibodies that bind to a glycodelin epitope of a disclosed antibody are further provided. Hybridoma cell lines that produce a glycodelin monoclonal antibody of the invention are also disclosed herein. Kits comprising one or more of the disclosed glycodelin monoclonal antibodies and for practicing the methods of the invention are further provided. Polypeptides comprising the amino acid sequence for a glycodelin epitope of a disclosed monoclonal glycodelin antibody and methods of using these polypeptides in the production of glycodelin antibodies are also encompassed by the present invention.

摘要翻译： 提供了一种用于诊断患者卵巢癌和用于鉴定患卵巢癌可能性增加的患者的组合物和方法。 组合物包括特异性结合糖链蛋白的新型单克隆抗体及其变体和片段。 进一步提供具有本发明的糖链蛋白抗体的结合特征的单克隆抗体和与所公开的抗体的糖链蛋白表位结合的单克隆抗体。 本文还公开了产生本发明的糖链蛋白单克隆抗体的杂交瘤细胞系。 进一步提供包含一种或多种所公开的糖链素单克隆抗体并用于实施本发明方法的试剂盒。 包含所公开的单克隆糖蛋白抗体的糖链蛋白表位的氨基酸序列的多肽和在制备糖链蛋白抗体中使用这些多肽的方法也包括在本发明中。

公开(公告)号：US08252904B2

公开(公告)日：2012-08-28

申请号：US12718693

申请日：2010-03-05

申请人： Robert L. Cheek ,  Eric P. Dixon ,  Timothy J. Fischer ,  John W. Groelke ,  Steven L. Knapp ,  Stephen G. Simkins ,  Clark M. Whitehead

发明人： Robert L. Cheek ,  Eric P. Dixon ,  Timothy J. Fischer ,  John W. Groelke ,  Steven L. Knapp ,  Stephen G. Simkins ,  Clark M. Whitehead

IPC分类号： C07K16/00 ,  C07K14/00 ,  C07K5/00 ,  G01N33/53

CPC分类号： C07K16/3069 ,  C07K16/18 ,  C07K2317/34 ,  G01N33/57449

摘要： Compositions and methods for diagnosing ovarian cancer in a patient and for identifying patients with an increased likelihood of having ovarian cancer are provided. The compositions include novel monoclonal antibodies, and variants and fragments thereof, that specifically bind to glycodelin. Monoclonal antibodies having the binding characteristics of a glycodelin antibody of the invention and monoclonal antibodies that bind to a glycodelin epitope of a disclosed antibody are further provided. Hybridoma cell lines that produce a glycodelin monoclonal antibody of the invention are also disclosed herein. The compositions find use in diagnostic methods as well as in screening methods for identifying patients having an increased likelihood of having ovarian cancer. Kits comprising one or more of the disclosed glycodelin monoclonal antibodies and for practicing the methods of the invention are further provided. Polypeptides comprising the amino acid sequence for a glycodelin epitope of a disclosed monoclonal glycodelin antibody and methods of using these polypeptides in the production of glycodelin antibodies are also encompassed by the present invention.

摘要翻译： 提供了一种用于诊断患者卵巢癌和用于鉴定患卵巢癌可能性增加的患者的组合物和方法。 组合物包括特异性结合糖链蛋白的新型单克隆抗体及其变体和片段。 还提供具有本发明的糖链蛋白抗体的结合特征的单克隆抗体和结合所公开的抗体的糖链蛋白表位的单克隆抗体。 本文还公开了产生本发明的糖链蛋白单克隆抗体的杂交瘤细胞系。 该组合物用于诊断方法以及用于鉴定具有增加卵巢癌可能性的患者的筛选方法。 进一步提供包含一种或多种所公开的糖链素单克隆抗体并用于实施本发明方法的试剂盒。 包含所公开的单克隆糖蛋白抗体的糖链蛋白表位的氨基酸序列的多肽和在制备糖链蛋白抗体中使用这些多肽的方法也包括在本发明中。

公开(公告)号：US20100227343A1

公开(公告)日：2010-09-09

申请号：US12718693

申请日：2010-03-05

申请人： Robert L. Cheek ,  Eric P. Dixon ,  Timothy J. Fischer ,  John W. Groelke ,  Steven L. Knapp ,  Stephen G. Simkins ,  Clark M. Whitehead

发明人： Robert L. Cheek ,  Eric P. Dixon ,  Timothy J. Fischer ,  John W. Groelke ,  Steven L. Knapp ,  Stephen G. Simkins ,  Clark M. Whitehead

IPC分类号： G01N33/53 ,  C07K16/18 ,  C07K5/117 ,  C07K14/00 ,  C07H21/04 ,  C12N5/16 ,  C12P21/08

CPC分类号： C07K16/3069 ,  C07K16/18 ,  C07K2317/34 ,  G01N33/57449

摘要： Compositions and methods for diagnosing ovarian cancer in a patient and for identifying patients with an increased likelihood of having ovarian cancer are provided. The compositions include novel monoclonal antibodies, and variants and fragments thereof, that specifically bind to glycodelin. Monoclonal antibodies having the binding characteristics of a glycodelin antibody of the invention and monoclonal antibodies that bind to a glycodelin epitope of a disclosed antibody are further provided. Hybridoma cell lines that produce a glycodelin monoclonal antibody of the invention are also disclosed herein. The compositions find use in diagnostic methods as well as in screening methods for identifying patients having an increased likelihood of having ovarian cancer. Kits comprising one or more of the disclosed glycodelin monoclonal antibodies and for practicing the methods of the invention are further provided. Polypeptides comprising the amino acid sequence for a glycodelin epitope of a disclosed monoclonal glycodelin antibody and methods of using these polypeptides in the production of glycodelin antibodies are also encompassed by the present invention.

摘要翻译： 提供了一种用于诊断患者卵巢癌和用于鉴定患卵巢癌可能性增加的患者的组合物和方法。 组合物包括特异性结合糖链蛋白的新型单克隆抗体及其变体和片段。 进一步提供具有本发明的糖链蛋白抗体的结合特征的单克隆抗体和与所公开的抗体的糖链蛋白表位结合的单克隆抗体。 本文还公开了产生本发明的糖链蛋白单克隆抗体的杂交瘤细胞系。 该组合物用于诊断方法以及用于鉴定具有增加卵巢癌可能性的患者的筛选方法。 进一步提供包含一种或多种所公开的糖链素单克隆抗体并用于实施本发明方法的试剂盒。 包含所公开的单克隆糖蛋白抗体的糖链蛋白表位的氨基酸序列的多肽和在制备糖链蛋白抗体中使用这些多肽的方法也包括在本发明中。

公开(公告)号：US08476033B2

公开(公告)日：2013-07-02

申请号：US13560080

申请日：2012-07-27

申请人： Robert L. Cheek ,  Eric P. Dixon ,  Timothy J. Fischer ,  John W. Groelke ,  Steven L. Knapp ,  Stephen G. Simkins ,  Clark M. Whitehead

发明人： Robert L. Cheek ,  Eric P. Dixon ,  Timothy J. Fischer ,  John W. Groelke ,  Steven L. Knapp ,  Stephen G. Simkins ,  Clark M. Whitehead

IPC分类号： G01N33/00 ,  C07K16/00

CPC分类号： C07K16/3069 ,  C07K16/18 ,  C07K2317/34 ,  G01N33/57449

摘要： Compositions and methods for diagnosing ovarian cancer in a patient and for identifying patients with an increased likelihood of having ovarian cancer are provided. The compositions include novel monoclonal antibodies, and variants and fragments thereof, that specifically bind to glycodelin. Monoclonal antibodies having the binding characteristics of a glycodelin antibody of the invention and monoclonal antibodies that bind to a glycodelin epitope of a disclosed antibody are further provided. Hybridoma cell lines that produce a glycodelin monoclonal antibody of the invention are also disclosed herein. Kits comprising one or more of the disclosed glycodelin monoclonal antibodies and for practicing the methods of the invention are further provided. Polypeptides comprising the amino acid sequence for a glycodelin epitope of a disclosed monoclonal glycodelin antibody and methods of using these polypeptides in the production of glycodelin antibodies are also encompassed by the present invention.

摘要翻译： 提供了一种用于诊断患者卵巢癌和用于鉴定患卵巢癌可能性增加的患者的组合物和方法。 组合物包括特异性结合糖链蛋白的新型单克隆抗体及其变体和片段。 进一步提供具有本发明的糖链蛋白抗体的结合特征的单克隆抗体和与所公开的抗体的糖链蛋白表位结合的单克隆抗体。 本文还公开了产生本发明的糖链蛋白单克隆抗体的杂交瘤细胞系。 进一步提供包含一种或多种所公开的糖链素单克隆抗体并用于实施本发明方法的试剂盒。 包含所公开的单克隆糖蛋白抗体的糖链蛋白表位的氨基酸序列的多肽和在制备糖链蛋白抗体中使用这些多肽的方法也包括在本发明中。

公开(公告)号：US20100227335A1

公开(公告)日：2010-09-09

申请号：US12718603

申请日：2010-03-05

申请人： Jeffrey P. Baker ,  Robert L. Cheek ,  Eric P. Dixon ,  Timothy J. Fischer ,  Steven L. Knapp ,  Stephen G. Simkins ,  Clark M. Whitehead

发明人： Jeffrey P. Baker ,  Robert L. Cheek ,  Eric P. Dixon ,  Timothy J. Fischer ,  Steven L. Knapp ,  Stephen G. Simkins ,  Clark M. Whitehead

IPC分类号： G01N33/574 ,  C07K16/18 ,  C12N5/12 ,  C07K14/435 ,  C07K7/08 ,  C07H21/04 ,  C12P21/04 ,  G01N33/53

CPC分类号： C07K16/40 ,  G01N33/57449 ,  G01N2333/96494

摘要： Compositions and methods for diagnosing ovarian cancer in a patient and for identifying patients with an increased likelihood of having ovarian cancer are provided. The compositions include novel monoclonal antibodies, and variants and fragments thereof, that specifically bind to MMP-7. Monoclonal antibodies having the binding characteristics of an MMP-7 antibody of the invention and monoclonal antibodies that bind to an MMP-7 epitope of a disclosed antibody are further provided. Hybridoma cell lines that produce an MMP-7 monoclonal antibody of the invention are also disclosed herein. The compositions find use in diagnostic methods as well as in screening methods for identifying patients having an increased likelihood of having ovarian cancer. Kits comprising one or more of the disclosed MMP-7 monoclonal antibodies and for practicing the methods of the invention are further provided. Polypeptides comprising the amino acid sequence for an MMP-7 epitope of a disclosed monoclonal MMP-7 antibody and methods of using these polypeptides in the production of MMP-7 antibodies are also encompassed by the present invention.

摘要翻译： 提供了一种用于诊断患者卵巢癌和用于鉴定患卵巢癌可能性增加的患者的组合物和方法。 组合物包括特异性结合MMP-7的新型单克隆抗体及其变体和片段。 进一步提供具有本发明的MMP-7抗体与结合所公开的抗体的MMP-7表位的单克隆抗体的单克隆抗体。 产生本发明的MMP-7单克隆抗体的杂交瘤细胞系也在本文中公开。 该组合物用于诊断方法以及用于鉴定具有增加卵巢癌可能性的患者的筛选方法。 还提供了包含一种或多种所公开的MMP-7单克隆抗体并用于实施本发明的方法的试剂盒。 包含所公开的单克隆MMP-7抗体的MMP-7表位的氨基酸序列的多肽和在产生MMP-7抗体中使用这些多肽的方法也包括在本发明中。

公开(公告)号：US20120141502A1

公开(公告)日：2012-06-07

申请号：US13265332

申请日：2010-04-20

申请人： Eric Dixon ,  Rainer Blaesius ,  Stephen Simkins ,  Steven L. Knapp ,  George Brough ,  Karen Lenz ,  Johannes Schweizer ,  Peter Lu ,  David Garman ,  Jon Silver ,  Charles Mahoney ,  Chamorro Somoza Diaz-Sarmiento

发明人： Eric Dixon ,  Rainer Blaesius ,  Stephen Simkins ,  Steven L. Knapp ,  George Brough ,  Karen Lenz ,  Johannes Schweizer ,  Peter Lu ,  David Garman ,  Jon Silver ,  Charles Mahoney ,  Chamorro Somoza Diaz-Sarmiento

IPC分类号： A61K39/395 ,  A61P31/20 ,  C40B30/04 ,  C12Q1/70 ,  C40B40/10

CPC分类号： G01N33/5748 ,  C07K16/084 ,  C07K2317/33 ,  C07K2317/34 ,  C07K2317/76 ,  G01N33/56983 ,  G01N2333/025 ,  G01N2469/10

摘要： The subject invention provides an antibody composition for detecting E6 protein of at least one HPV strain in a sample. The subject antibodies may be used to detect oncogenic HPV E6 proteins in a sample, and the antibodies find use in a variety of diagnostic and therapeutic applications, including methods of diagnosing and treating cancer. Kits for performing the subject methods and containing the subject antibodies are also provided. Also disclosed in the present invention is a method of generating an antibody that specifically binds to amino-terminus of E6 proteins of at least two HPV strains.

摘要翻译： 本发明提供了用于检测样品中至少一种HPV病毒株的E6蛋白的抗体组合物。 受试抗体可用于检测样品中的致癌HPV E6蛋白，并且抗体可用于多种诊断和治疗应用，包括诊断和治疗癌症的方法。 还提供了用于进行受试方法并含有目标抗体的试剂盒。 本发明还公开了产生特异性结合至少两种HPV病毒株的E6蛋白的氨基末端的抗体的方法。