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公开(公告)号：US20130288267A1
公开(公告)日：2013-10-31
申请号：US13923646
申请日：2013-06-21
Applicant: Roche Diagnostics Operations, Inc.
Inventor： Michael Gerg , Dieter Heindl , Alfred Mertens , Christoph Rutz , Michael Schraeml , Monika Soukupova , Claudio Sustmann , Michael Tacke , Jan van Dieck
IPC: C07K16/46
CPC classification number: C07K16/468 , C07K16/18 , C07K16/2863 , C07K16/32 , C07K16/44 , C07K2317/31 , C07K2317/34 , C07K2317/55 , C07K2317/92 , C07K2317/94 , G01N33/6857
Abstract: A bivalent binding agent, capable of binding a polypeptide dimer, consisting of two monovalent binders linked to each other via a linker, the first monovalent binder binds an epitope of a first target polypeptide comprised in said dimer and the second monovalent binder binds to an epitope of a second target polypeptide comprised in said dimer. Each monovalent binder has a Kdiss in the range of 5×10−3/sec to 10−4/sec, and the bivalent binding agent has a Kdiss of 3×10−5/sec or less. Methods of making and using such bivalent binding agent in histological staining procedures are also disclosed.
Abstract translation: 能够结合多肽二聚体的二价结合剂，其由通过接头彼此连接的两个单价结合物组成，所述第一单价键合物结合包含在所述二聚体中的第一目标多肽的表位，并且所述第二单价键合物结合表位 的包含在所述二聚体中的第二目标多肽。 每个一价粘合剂的Kdiss在5×10-3 /秒至10-4 /秒的范围内，二价结合剂的Kdiss为3×10-5 /秒或更小。 还公开了在组织学染色方法中制备和使用这种二价结合剂的方法。
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公开(公告)号：US10982007B2
公开(公告)日：2021-04-20
申请号：US15465439
申请日：2017-03-21
Applicant: Roche Diagnostics Operations, Inc.
Inventor： Michael Gerg , Dieter Heindl , Christian Klein , Alfred Mertens , Volker Schmid , Michael Schraeml , Monika Soukupova , Michael Tacke
IPC: C07K16/46 , G01N33/531
Abstract: A bivalent binding agent having a first monovalent binder that binds to a polypeptide epitope of a target polypeptide, a second monovalent binder that binds to a posttranslational polypeptide modification on the target polypeptide and a linker. Further disclosed are methods for the detection of a posttranslationally modified target polypeptide, for making the disclosed bivalent binding agent, and for use of the disclosed bivalent binding agent in histological staining procedures.
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公开(公告)号：US20170275380A1
公开(公告)日：2017-09-28
申请号：US15465379
申请日：2017-03-21
Applicant: Roche Diagnostics Operations, Inc.
Inventor： Michael Gerg , Dieter Heindl , Alfred Mertens , Christoph Rutz , Michael Schraemi , Monika Soukupova , Claudio Sustmann , Michael Tacke , Jan van Dieck
CPC classification number: C07K16/468 , C07K16/18 , C07K16/2863 , C07K16/32 , C07K16/44 , C07K2317/31 , C07K2317/34 , C07K2317/55 , C07K2317/92 , C07K2317/94 , G01N33/6857
Abstract: A bivalent binding agent, capable of binding a polypeptide dimer, consisting of two monovalent binders linked to each other via a linker, the first monovalent binder binds an epitope of a first target polypeptide comprised in said dimer and the second monovalent binder binds to an epitope of a second target polypeptide comprised in said dimer. Each monovalent binder has a Kdiss in the range of 5×10−3/sec to 10−4/sec, and the bivalent binding agent has a Kdiss of 3×10−5/sec or less. Methods of making and using such bivalent binding agent in histological staining procedures are also disclosed.
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公开(公告)号：US20170275381A1
公开(公告)日：2017-09-28
申请号：US15465439
申请日：2017-03-21
Applicant: Roche Diagnostics Operations, Inc.
Inventor： Michael Gerg , Dieter Heindl , Christian Klein , Alfred Mertens , Volker Schmid , Michael Schraemi , Monika Soukupova , Michael Tacke
IPC: C07K16/46 , G01N33/531
CPC classification number: C07K16/468 , G01N33/531
Abstract: A bivalent binding agent having a first monovalent binder that binds to a polypeptide epitope of a target polypeptide, a second monovalent binder that binds to a posttranslational polypeptide modification on the target polypeptide and a linker. Further disclosed are methods for the detection of a posttranslationally modified target polypeptide, for making the disclosed bivalent binding agent, and for use of the disclosed bivalent binding agent in histological staining procedures.
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